Structure-function studies of tryptophan mutants of equinatoxin II, a sea anemone pore-forming protein

Biochemical Journal

Volumn 346, Issue 1, 2000, Pages 223-232

  Malovrh, Petra ^a   Barlič, Ariana ^a   Podlesek, Zdravko ^a   Maček, Peter ^a   Menestrina, Gianfranco ^b   Anderluh, Gregor ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

^b CNR   (Italy)

Author keywords

Actinia equina; Actinoporin; Fluorescence; FTIR spectroscopy; Lipid membranes

Indexed keywords

EQUINATOXIN; MUTANT PROTEIN; NITROGEN; PROTON; TRYPTOPHAN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FLUORESCENCE; FOURIER TRANSFORMATION; HEMOLYSIS; HYDROPHOBICITY; LIPID MEMBRANE; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SEA ANEMONE; STRUCTURE ACTIVITY RELATION;

ACRYLAMIDE; AMINO ACID SEQUENCE; ANIMALS; BROMOSUCCINIMIDE; CATTLE; CELL DEATH; CNIDARIAN VENOMS; CYTOTOXINS; ERYTHROCYTES; FLUORESCENCE; HEMOLYSIS; LIPOSOMES; MOLECULAR SEQUENCE DATA; MUTATION; NITROGEN; PORINS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEA ANEMONES; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; STRUCTURE-ACTIVITY RELATIONSHIP; TRYPTOPHAN; WATER;

EID: 0034652361     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3460223     Document Type: Article

References (42)

1. 1 Gouaux, E. (1997) Curr. Opin. Struct. Biol. 7, 566-573
2. 2 Turk, T. (1991) J. Toxicol. Toxin. Rev. 10, 223-262
3. 3 Maček, P. (1992) FEMS Microbiol. Immunol. 105, 121-130
4. 4 Maček, P., Zecchini, M., Pederzolli, C., Dalla Serra, M. and Menestrina, G. (1995) Eur. J. Biochem. 234, 329-335
5. 5 Anderluh, G., Križaj, I., Štrukelj, B., Gubenšek, F., Maček, P. and Pungerčar, J. (1999) Toxicon 37, 1391-1401
6. 6 Zorec, R., Tester, M., Maček, P. and Mason, W. T. (1990) J. Membr. Biol. 118, 243-249
7. 7 Belmonte, G., Pederzolli, C., Maček, P. and Menestrina, G. (1993) J. Membr. Biol. 131, 11-22
8. 8 Anderluh, G., Barlič, A., Podlesek, Z. Maček, P., Pungerčar, J., Gubenšek, F., Zecchini, M., Serra, M. D. and Menestrina, G. (1999) Eur. J. Biochem. 263, 128-136
9. 9 Belmonte, G., Menestrina, G., Pederzolli, C., Križaj, I., Gubenšek, F., Turk, T. and Maček, P. (1994) Biochim. Biophys. Acta 1192, 197-204
10. 10 Anderluh, G., Pungerčar, J., Križaj, I., Štrukelj, B., Gubenšek, F. and Maček, P. (1997) Protein Eng. 10, 751-755
11. 11 Menestrina, G., Cabiaux, V. and Tejuca, M. (1999) Biochem. Biophys. Res. Commun. 254, 174-180
12. 12 Weiss, M. S., Abele, U., Weckesser, J., Welte, W., Schiltz, E. and Schulz, G. E. (1991) Science 254, 1627-1630
13. 13 Cowan, S. W., Schirmer, T., Rummel, G., Steiert, M., Ghosh, R., Pauplit, R. A., Jansonius, J. N. and Rosenbusch, J. P. (1992) Nature (London) 358, 727-733
14. 14 Doyle, D. A., Cabral, J., M., Pfuetzner, A. K., Gulbis, J. M., Cohen, S. L., Chait, B. T. and MacKinnon, R. (1998) Science 280, 69-77
15. 15 Forst, D., Welte, W., Wacker, T. and Diederichs, K. (1998) Nat. Struct. Biol. 5, 37-46
16. 16 Wallace, B. A. (1998) J. Struct. Biol. 121, 123-141
17. 17 Song, L., Hobaugh, M. R., Shustak, C., Cheley, S., Bayley, H. and Gouaux, J. E. (1996) Science 274, 1859-1866
18. 18 Gouaux, E. (1998) J. Struct. Biol. 121, 110-122
19. 19 Turk, T., Maček, P. and Gubenšek, F. (1992) Biochim. Biophys. Acta 1119, 1-4
20. 20 Pazos, I. F., Alvarez, C., Lanio, M. E., Martinez, D., Morera, V., Lissi, E. A. and Campos, A. M. (1998) Toxicon 36, 1383-1393
21. 21 Khoo, H. E., Fong, C. L., Yuen, R. and Chen, D. S. (1997) Biochem. Biophys. Res. Commun. 232, 422-426
22. 22 Anderluh, G., Pungerčar, J., Štrukelj, B., Maček, P. and Gubenšek, F. (1996) Biochem. Biophys. Res. Commun. 220, 437-442
23. 23 Michael, S. F. (1994) BioTechniques 16, 410-412
24. 24 Maček, P. and Lebez, D. (1988) Toxicon 26, 441-451
25. 25 Perkins, S. J. (1986) Eur. J. Biochem. 157, 169-180
26. 26 Lakowicz, J. R. (1983) Principles of Fluorescence Spectroscopy, Plenum Press, New York
27. 27 Eftink, M. R. and Ghiron, C. A. (1981) Anal. Biochem. 114, 199-227
28. 28 Harrick, N. J. (1967) Internal Reflection Spectroscopy, Harrick Scientific Corporation, Ossining, New York
29. 29 Menestrina, G. (2000) in Bacterial Toxins, Methods and Protocols (Holst, O., ed.), Humana Press, Totowa, NJ, in the press
30. 30 Susi, H. and Byler, D. M. (1986) Methods Enzymol. 130, 290-311
31. 31 Tatulian, S. A., Hinterdorfer, P., Baber, G. and Tamm, L. K. (1995) EMBO J. 14, 5514-5523
32. 32 Tamm, L. K. and Tatulian, S. A. (1997) Q. Rev. Biophys. 30, 365-429
33. 33 Jackson, M. and Mantsch, H. H. (1995) Crit. Rev. Biochem. Mol. Biol. 30, 95-120
34. 34 Poklar, N., Lah, J., Salobir, M., Maček, P. and Vesnaver, G. (1997) Biochemistry 36, 14345-14352
35. 35 Wu, P. and Brand, L. (1994) Anal. Biochem. 218, 1-13
36. 36 Freskgard, P. O., Martensson, L. G., Jonasson, P., Jonsson, B. H. and Carlsson, U. (1994) Biochemistry 33, 14281-14288
37. 37 Woody, R. W. (1994) Eur. Biophys. J. 23, 253-262
38. 38 Goormaghtigh, E., Cabiaux, V. and Ruysschaert, J.-M. (1994) Subcell. Biochem. 23, 363-403
39. 39 Doyle, J. W. and Kem, W. R. (1989) Biochim. Biophys. Acta 987, 181-186
40. 40 Lesieur, C., Vecsey-Semjen, L., Abrami, L., Fivaz, M. and Van der Goot, F. G. (1997) Mol. Membr. Biol. 14, 45-64
41. 41 Rossjohn, J., Feil, S. C., McKinstry, W. J., Tweten, R. K. and Parker, M. W. (1997) Cell 89, 685-692
42. 42 Sekino-Suzuki, N., Nakamura, M., Mitsui, K. I. and Ohno-Iwashita, Y. (1996) Eur. J. Biochem. 241, 941-947