Characterization of pK(a) values and titration shifts in the cytotoxic ribonuclease α-sarcin by NMR. Relationship between electrostatic interactions, structure, and catalytic function

Biochemistry

Volumn 37, Issue 45, 1998, Pages 15865-15876

  Pérez Cañadillas, José Manuel ^a   Campos Olivas, Ramón ^a,c   Lacadena, Javier ^b   Martínez Del Pozo, Alvaro ^b   Gavilanes, José G ^b   Santoro, Jorge ^a   Rico, Manuel ^a   Bruix, Marta ^a  

^a INSTITUTO DE ESTRUCTURA DE LA MATERIA   (Spain)

^b UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^c UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA SARCIN; RIBONUCLEASE; 2',3'-CYCLIC GMP; ALPHA-SARCIN; CYTOTOXIN; FUNGAL PROTEIN; GUANINE NUCLEOTIDE; GUANOSINE 2',3' PHOSPHATE; HISTIDINE; PROTON; RIBONUCLEASE T1;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; ELECTRICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; IONIZATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PKA; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; STRUCTURE ACTIVITY RELATION; TITRIMETRY; ASPERGILLUS; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; ENZYME ACTIVATION; MACROMOLECULE; METABOLISM; NUCLEAR MAGNETIC RESONANCE; PH;

ASPERGILLUS; CATALYSIS; CYTOTOXINS; ELECTROSTATICS; ENDORIBONUCLEASES; ENZYME ACTIVATION; FUNGAL PROTEINS; GUANINE NUCLEOTIDES; HISTIDINE; HYDROGEN-ION CONCENTRATION; MACROMOLECULAR SUBSTANCES; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTONS; RIBONUCLEASE T1; TITRIMETRY;

EID: 0032505872     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi981672t     Document Type: Article

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