Analysis of solid-phase reactions: Product identification and quantification by use of UV-chromophore-containing dual-linker analytical contructs

Angewandte Chemie - International Edition

Volumn 39, Issue 18, 2000, Pages 3293-3296

  Williams, Geoff M ^a   Carr, Robin A E ^b   Congreve, Miles S ^a   Kay, Corinne ^b   McKeown, Stephen C ^b   Murray, Peter J ^a,c   Scicinski, Jan J ^a   Watson, Stephen P ^b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c OSI Pharmaceuticals   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHEMICAL ANALYSIS; CHROMATOPHORE; MASS SPECTROMETRY; QUANTITATIVE ASSAY; REACTION ANALYSIS; SOLID; ULTRAVIOLET RADIATION;

EID: 0034665434     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/1521-3773(20000915)39:18<3293::AID-ANIE3293>3.0.CO;2-B     Document Type: Article

References (15)

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6. Dual-linker analytical constructs incorporating the dansyl and dahsyl groups have also been prepared and their properties studied as both UV chromophores and for fluorescence detection: see A. Zaramella, N. Conti, M. Dal Gin, A. Paio, P. Seneci, S. Gehanne, Anal. Chem. submitted.
7. The three-carbon-chain tether was found to be the minimum length necessary to avoid fragmentation and loss of anthracene during MS analysis.
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9. Commercially available from Peakdale Fine Chemicals.
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12. 3): δ = 7.7 (m, 4H) for 11a; 5.7 and 4.9 (m, 3H) for 11b: 2.9 (s, 6H) for 11c; 0.9 (s, 9H) for 11d.
13. In addition by construction of a standard curve using 9-methylanthracene and its use as an internal standard, the absolute quantification of the resin-bound products can be assessed. See Supporting Information.
14. S. I. Klein, B. F. Molino, M. Czekaj, C. J. Gardner, V. Chu, K. Brown, R. D. Sabatino, J. S. Bostwick, C. Kasiewski, R. Bentley, V. Windisch, M. Perrone, C. T. Dunwiddie, R. J. Leadley, J. Med. Chem. 1998, 41, 2492.
15. Careful examination of the mass spectral data of each intermediate analytical fragment indicated that no unexpected ion doublets were present, suggesting that the anthracene group was stable to all of the reaction conditions for the synthesis.