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12a and appropriate commercially available reagents. As noted, the modular strategy allows for various procedures to create equally functional constructs.
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All MALDI mass spectra were generated on a Ciphergen Biosystems Massphoresis System time-of-flight mass spectrometer. Typical resolution (m/Δm, where Δm = full width at half maximum) for these samples on this instrument was 100-200. All spectra were calibrated internally on matrix peaks.
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38
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0342933815
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note
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+ for any given substrate. Separate solution photolyses (354 nm, DMF, 1h) followed by ESI-MS analysis, along with other experiments, have indicated that these peaks are an artifact of the MALDI process. Our experiments have also shown that the extent of adduct formation is highly dependent on a combination of factors including the chemical nature of the substrate and photocleavable link as well as the MALDI matrix used.
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