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Volumn 289, Issue 5477, 2000, Pages 300-303

Requirement of the spindle checkpoint for proper chromosome segregation in budding yeast meiosis

Author keywords

[No Author keywords available]

Indexed keywords

ANAPHASE; ARTICLE; CENTROMERE; CHROMOSOME SEGREGATION; CHROMOSOME SIZE; DOWN SYNDROME; MEIOSIS; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; YEAST;

EID: 0034647893     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.289.5477.300     Document Type: Article
Times cited : (186)

References (45)
  • 3
    • 0032512748 scopus 로고    scopus 로고
    • L. H. Hwang et al., Science 279, 1041 (1998).
    • (1998) Science , vol.279 , pp. 1041
    • Hwang, L.H.1
  • 10
    • 0342479264 scopus 로고    scopus 로고
    • note
    • GFP-Lacl expression driven by CVC1 promoter, pAFS 152 (38).
  • 11
    • 0343348780 scopus 로고    scopus 로고
    • note
    • If only one homolog is marked with GFP, normal segregation of the two sister chromatids in meiosis II will produce two spores that have a single copy of the GFP-marked chromosomes. Nondisjunction in meiosis II will lead to a single spore that contains two copies of the GFP marked chromosome. The measured frequencies of nondisjunction in wild-type strains are higher than those reported from genetic tests (39). We believe that this discrepancy is due to a low frequency of recombination events that reduce the number of Lac operator repeats below the threshold required for detection.
  • 12
    • 0342913560 scopus 로고    scopus 로고
    • note
    • All strains are in the W303 background, unless otherwise noted: MAT a/MATα, ura3-1 leu2, 3-172, his3-11, trp1-1, ade2-1, can1-100. All mutations are homozygous unless otherwise noted. GFP tagging of chromosome IV (MAS 386, 327; wild-type and mad7Δ, respectively) and III (MAS 401, 424) is described in (9). Strains MAS 559, 561 were constructed by integrating pMAS72 near the centromere of chromosome VII. Plasmid pMAS72 contains a Bam Hl-Sac I fragment spanning nucleotides 478360 through 479162 of chromosome VII in plasmid pAFS149 (38) which contains 128 repeats of LacO. Strains MAS 656, 658 were constructed by integrating pMAS79 near the centromere of chromosome VIII. Plasmid pMAS79 contains a Bam Hl-Sac I fragment spanning nucleotides 123761 through 124110 on chromosome VIII in pAFS149. All experiments were repeated at least twice with similar results. In all experiments, at least 200 cells were counted.
  • 13
    • 0343784503 scopus 로고    scopus 로고
    • note
    • We used the SK1 strain background for all experiments requiring a rapid, synchronous meiosis. Synchronous sporulation in 2% potassium acetate as in (74). All SK1 strains derived from NKY 611:leu2::hisG ura3, ho::LYS2. lys2. SK1 wild-type: MAS 120, SK1 mad2A: MAS 403. SK1 mad2Δ mutants also undergo meiosis I nondisjunction, but at higher levels than W303.
  • 18
    • 0343784478 scopus 로고    scopus 로고
    • note
    • 4 formed dyads at reduced efficiency compared to those sporulated in the absence of copper. Sister chromatid separation occurs in only 4% of divisions for both wild type and mad2Δ.
  • 19
    • 0342913518 scopus 로고    scopus 로고
    • note
    • SK1 cells were sporulated for 4 hours at room temperature in 2% potassium acetate as in (13), then resuspended in 2% potassium acetate plus 60 μg/ml benomyl, prepared by adding a 30 mg/ml stock of benomyl in dimethyl sulfoxide to boiling media and slowly cooling to room temperature. Cells were fixed in 40% ethanol and 0.1 M sorbitol.
  • 21
    • 0033538518 scopus 로고    scopus 로고
    • F. Klein et al., Cell 98, 91 (1999).
    • (1999) Cell , vol.98 , pp. 91
    • Klein, F.1
  • 24
    • 0343784421 scopus 로고    scopus 로고
    • note
    • We determined the position of chromosome III homologs on the long meiosis I spindle in spo11Δ (SK1 strain MAS 645: LacO:LEU2 GFP-Lacl spo11Δ) by indirect immunofluorescence against Tub1 and GFP-Lacl. We find that the marked centromere is always pulled to a spindle pole, suggesting that homologs are attached to the spindle.
  • 38
    • 0342913511 scopus 로고    scopus 로고
    • unpublished data
    • A. Straight, unpublished data.
    • Straight, A.1
  • 42
    • 0342479163 scopus 로고    scopus 로고
    • note
    • Plasmid pMAS68 was constructed by inserting a fragment of the TUB1 promoter into pAFS160 (38) which contains a fusion between TUB4 and GFP. Plasmid was integrated at URA3 to create SK1 strain MAS 601.
  • 43
    • 0343348684 scopus 로고    scopus 로고
    • note
    • SKI wild-type (MAS608), spo11Δ (MAS613), spo11Δ mad2Δ (MAS613), and spo11Δ spo13Δ (MAS 659) homozygous diploids all express the Pds1 protein tagged with 18 copies of the Myc epitope (47).
  • 45
    • 0342913504 scopus 로고    scopus 로고
    • note
    • We thank M. Winey for sharing unpublished information and S. Roeder, S. Hawley, B. Nicklas, and members of the Murray lab for critical reading of the manuscript. We are grateful to the Kleckner lab for SK1 strains. Supported by grants from NIH and the Human Frontiers In Science Program.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.