Conformations of kinesin: Solution vs. crystal structures and interactions with microtubules

European Biophysics Journal

Volumn 27, Issue 5, 1998, Pages 455-465

  Marx, A ^a   Thormahlen M ^a   Muller J ^a   Sack, S ^a   Mandelkow, E M ^a   Mandelkow, E ^a  

^a DESY   (Germany)

Author keywords

Cell motility; Kinesin; Microtubules; Motor proteins; Small angle X ray scattering; X ray crystallography

Indexed keywords

KINESIN; MICROTUBULE PROTEIN;

ARTICLE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; MICROTUBULE; NONHUMAN; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

ANIMALS; BINDING SITES; BIOPHYSICS; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; KINESIN; MICROTUBULES; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SCATTERING, RADIATION; SOLUTIONS;

EID: 0345055326     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490050156     Document Type: Article

References (45)

1. Amos LA, Klug A (1974) Arrangement of subunits in flagellar microtubules. J Cell Sci 14:523-549
2. Arnal I, Metoz F, Debonis S, Wade RH (1996) Three-dimensional structure of functional motor proteins on microtubules. Curr Biol 6:1265-1270
3. Arnal I, Wade RH (1995) How does taxol stabilize microtubules. Curr Biol 5:900-908
4. Brady ST (1995) A kinesin medley - biochemical and functional heterogeneity. Trends Cell Biol 5:159-164
5. Brünger AT (1992) X-PLOR version 3.1. A system for X-ray crystallography and NMR. Yale University Press, New Haven, CT, USA
6. Cambillau C, Roussel A, Inisan AG, Knoops-Mouthuy E (1996) Turbo-Frodo version 5.5, AFMB-IFRC1 chemin. Aiguier, Marseille, France
7. Cole DG, Scholey JM (1995) Structural variations among the kinesins. Trends Cell Biol 5:259-262
8. Collaborative Computing Project No. 4 (1994) CCP4 suite of programs. Acta Cryst. D 50, 760-763
9. Crevel IMTC, Lockhart A, Cross RA (1996) Weak and strong states of kinesin and ncd. J Mol Biol 257:66-76
10. Cross RA (1997) The natural economy of kinesin. Curr Biol 7:R631-R633
11. Gilbert SP, Moyer ML, Johnson KA (1998) Alternating site mechanism of the kinesin ATPase. Biochemistry 37:792-799
12. Hirokawa N (1996) The molecular mechanism of organelle transport along microtubules: the identification and characterization of KIFs (kinesin superfamily proteins). Cell Struct Funct 21:357-367
13. Hirose K, Lockhart A, Cross RA, Amos LA (1996) Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules. Proc Natl Acad Sci USA 93:9539-9544
14. Hoenger A, Sack S, Thormählen M, Marx A, Müller J, Gross H, Mandelkow E (1998) Image reconstructions of microtubules decorated with monomeric and dimeric kinesins: comparison with X-ray structure and implications for motility. J Cell Biol 141:419-430
15. Holmes KC (1997) The swinging lever-arm hypothesis of muscle contraction. Curr Biol 7: R112-R118
16. Hyman AA, Chretien D, Arnal I, Wade RH (1995) Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue guanylyl-(α,β)-methylene-diphosphonate. J Cell Biol 128:117-125
17. Jiang W, Stock MF, Li X, Hackney DD (1997) Influence of the kinesin neck domain on dimerization and ATPase kinetics. J Biol Chem 272:7626-7632
18. Kleywegt GJ (1996) Use of non-crystallographic symmetry in protein structure refinement. Acta Cryst D 52:842-857
19. Koch M, Bordas J (1983) X-ray diffraction and scattering on disordered systems using synchrotron radiation. Nucl Instrum Methods 208:461-469
20. Kozielski F (1997) Die Röntgenstrukturanalyse des Motorproteins Kinesin. Ph.D. thesis, Universität Hamburg, Germany
21. Kozielski F, Sack S, Marx A, Thormählen M, Schönbrunn E, Biou V, Thompson A, Mandelkow E-M, Mandelkow E (1997a) The crystal structure of dimeric kinesin and implications for microtubule-dependent motility. Cell 91:985-994
22. Kozielski F, Schönbrunn E, Sack S, Müller J, Brady S, Mandelkow E (1997b) Crystallization and preliminary X-ray analysis of monomeric and dimeric kinesin motor domains. J Struct Biol 119:28-34
23. Kraulis PJ (1991) Molscript: a program to produce both detailed and schematic plots of protein structures. J Appl Cryst 24:946-950
24. Kull FJ, Sablin E, Lau P, Fletterick R, Vale R (1996) Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature 380:550-554
25. Lockhart A, Crevel IMTC, Cross RA (1995) Kinesin and ncd bind through a single head to microtubules and compete for a shared mt binding-site. J Mol Biol 249:763-771
26. Ma YZ, Taylor EW (1997) Interacting head mechanism of microtubule-kinesin ATPase. J Biol Chem 272:724-730
27. Mandelkow E, Thomas J, Cohen C (1977) Microtubule structure at low resolution by x-ray diffraction. Proc Natl Acad Sci USA 74:3370-3374
28. Mandelkow E-M, Herrmann M, Ruehl U (1985) Tubulin domains probed by limited proteolysis and subunit-specific antibodies. J Mol Biol 185:311-327
29. Mandelkow E, Song Y-H, Mandelkow E-M (1995) The microtubule lattice - dynamic instability of concepts. Trends Cell Biol 5:262-266
30. Murray JM (1991) Structure of flagellar microtubules. Int Rev Cytol 125:47-93
31. Murshudov G, Vagin A, Dodson E (1996) In: Dodson E, Moore M, Ralph A, Bailey S (eds) Macromolecular refinement. Proceedings of the CCP4 Study Weekend. SERC Daresbury Laboratory, Warrington, UK, pp 93-104
32. Navazza J (1994) AMoRe: an automated package for molecular replacement. Acta Cryst A 50: 157-163
33. Otwinowski Z, Minor W (1997) Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol 276:307-326
34. Rayment I (1996) Kinesin and myosin: molecular motors with similar engines. Structure 4:501-504
35. Sablin EP, Kull FJ, Cooke R, Vale RD, Fletterick RJ (1996) Crystal structure of the motor domain of the kinesin-related motor ncd. Nature 380:555-559
36. Sack S, Müller J, Marx A, Thormählen M, Mandelkow E-M, Brady ST, Mandelkow E (1997) X-ray structure of motor and neck domain from rat brain kinesin. Biochemistry 36:16155-16165
37. Song Y-H, Mandelkow E (1993) Recombinant kinesin motor domain binds to β-tubulin and decorates microtubules with a B surface lattice. Proc Natl Acad Sci USA 90:1671-1675
38. Sosa H, Dias DP, Hoenger A, Whittaker M, Wilson-Kubalek E, Sablin E, Fletterick RJ, Vale RD, Milligan RA (1997) A model for the microtubule-ncd motor protein complex obtained by cryoelectron microscopy and image-analysis. Cell 90:217-224
39. Svergun D, Richard S, Koch MHJ, Sayers Z, Kuprin S, Zaccai G (1998) Protein hydration in solution: Experimental observation by x-ray and neutron scattering. Proc Natl Acad Sci USA 95:2267-2272
40. Thormählen M, Marx A, Müller SA, Song Y-H, Mandelkow E-M, Aebi U, Mandelkow E (1998a) Interaction of monomeric and dimeric kinesin with microtubules. J Mol Biol 275:795-809
41. Thormählen M, Marx A, Sack S, Mandelkow E-M (1998b) The coiled-coil helix in the neck of kinesin. J Struct Biol (in press)
42. Vale RD (1996) Switches, latches, and amplifiers: common themes of G proteins and molecular motors. J Cell Biol 135:291-302
43. Vale RD, Coppin CM, Malik F, Kull FJ, Milligan RA (1994) Tubulin GTP hydrolysis influences the structure, mechanical properties, and kinesin-driven transport of microtubules. J Biol Chem 269:23769-23775
44. Vale RD, Fletterick RJ (1997) The design plan of kinesin motors. Annu Rev Cell Dev Biol 13:745-777
45. Woehlke G, Ruby AK, Hart CL, Ly B, Hom-Booher N, Vale RD (1997) Microtubule interaction site of the kinesin motor. Cell 90:207-216