Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-α

Journal of Molecular Biology

Volumn 297, Issue 5, 2000, Pages 1183-1194

  Fontes, Marcos R M ^a   Teh, Trazel ^a   Kobe, Bostjan ^a  

^a ST VINCENT'S INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

Importin karyopherin ; Nuclear localization sequence (NLS) recognition; Nucleoplasmin NLS; Simian virus 40 (SV40) large T antigen NLS; X ray crystal structure

Indexed keywords

CARRIER PROTEIN; KARYOPHERIN; NUCLEOPLASMIN; RECEPTOR PROTEIN; VIRUS LARGE T ANTIGEN;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN STRUCTURE; RECEPTOR BINDING; SIMIAN VIRUS;

EID: 0034646565     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3642     Document Type: Article

References (44)

1. Transport pathways of macromolecules between the nucleus and the cytoplasm
2. Free R value: A novel statistical quantity for assessing the accuracy of crystal structures
3. Crystallography and NMR system: A new software suite for macromolecular structure determination
4. Ribbons
5. The CCP4 suite: Programs for protein crystallography
6. Sequence requirements for synthetic peptide-mediated translocation to the nucleus
7. RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import
8. Pictures in cell biology. Structures of nuclear-transport components
9. Extensive mutagenesis of the nuclear location signal of simian virus 40 large-T antigen
10. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha
11. Nuclear targeting sequences-a consensus?
12. Nuclear import: A tale of two sites
13. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities
14. Nuclear transport: What a kary-on
15. A 41 amino acid motif in importin-alpha confers binding to importin-beta and hence transit into the nucleus
16. In or out? Regulating nuclear transport
17. The protein kinase CK2 site (Ser111/112) enhances recognition of the simian virus 40 large T-antigen nuclear localization sequence by importin
18. Regulation of protein transport to the nucleus: Central role of phosphorylation
19. A macromolecule modeling environment
20. Improved methods for building protein models in electron density maps and the location of errors in these models
21. Sequence requirements for nuclear location of simian virus 40 large-T antigen
22. A short amino acid sequence able to specify nuclear location
23. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha
24. Active site-directed protein regulation
25. Nuclear protein transport pathways
26. Evidence for distinct substrate specificities of importin alpha members in nuclear protein import
27. Yeast Srp1, a nuclear protein related to Drosophila and mouse pendulin, is required for normal migration, division, and integrity of nuclei during mitosis
28. Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen
29. PROCHECK: A program to check the stereochemical quality of protein structures
30. Nuclear transport: Randy couples
31. Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids
32. Snail mail to the nucleus
33. Isoform-specific purification and substrate specificity of the 5'-AMP-activated protein kinase
34. Nuclear import and export: Transport factors, mechanisms and regulation
35. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence
36. Processing of x-ray diffraction data collected in oscillation mode
37. A repeating amino acid motif shared by proteins with diverse cellular roles
38. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: Identification of a class of bipartite nuclear targeting sequence
39. Nuclear import and export of proteins: The molecular basis for intracellular signalling
40. Switching affinities in nuclear trafficking
41. Getting across the nuclear pore complex
42. Crystallization of importin α, the nuclear import receptor
43. The conserved amino-terminal domain of hSRP1 alpha is essential for nuclear protein import