Nuclear import and export: Transport factors, mechanisms and regulation

Critical Reviews in Eukaryotic Gene Expression

Volumn 9, Issue 2, 2000, Pages 89-106

  Moroianu, Junona ^a  

^a BOSTON COLLEGE   (United States)

Author keywords

Karyopherins; Nuclear export signal (NES); Nuclear localization signal (NLS); Nuclear pore complex (NPC); Nucleoporins; Ran GTPase

Indexed keywords

CARRIER PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; KARYOPHERIN; M PROTEIN; NUCLEOPORIN; REV PROTEIN;

CELL NUCLEUS; CELL NUCLEUS MEMBRANE; COMPLEX FORMATION; EUKARYOTIC CELL; INTRACELLULAR TRANSPORT; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN TRANSPORT; REGULATORY MECHANISM; REVIEW;

EUKARYOTA; NES;

EID: 0033953876     PISSN: 10454403     EISSN: None     Source Type: Journal    
DOI: 10.1615/critreveukargeneexpr.v9.i2.10     Document Type: Review

References (149)

1. Adam EJH, Adam SA (1994): Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. J Cell Biol 125:547-555.
2. Aitchison JD, Blobel G, Rout MP (1996): Kap104p: A karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science 274:624-627.
3. Albertini M, Pemberton LF, Rosenblum JS, Blobel G (1998): A novel nuclear import pathway for the transcription factor TFIIS. J Cell Biol 143:1447-1455.
4. Arts GJ, Fornerod M, Mattaj IW (1998): Identification of a nuclear export receptor for tRNA. Curr Biol 8:305-314.
5. Beals CR, Clipstone NA, Ho SN, Crabtree GR (1997): Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction. Genes Dev 11:824-834.
6. Becker J, Dickmanns A, Koerner C, Herrman C, Wittinghofer A (1998): Identification of a new Ran binding domain in Nup153 and RanBP2. Abstr. P38, Cold Spring Harbor Meeting on "Dynamic Organization of Nuclear Function".
7. Bischoff FR, Ponstingl H (1991): Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature 354:80-82.
8. Bischoff FR, Kreber H, Kempf T, Hermes I, Ponstingl H (1995a): Human RanGTPase-activating protein RanGAP1 is a homologue of yeast RNA1p involved in mRNA processing and transport. Proc Natl Acad Sci USA 92:1749-1753.
9. Bischoff R, Krebber H, Smirnova E, Dong W, Ponstingl H (1995b): Coactivation of RanGTPase and inhibition of GTP dissociation by Ran-GTP protein RanBP1. EMBO J 14:705-715.
10. Bonifaci N, Moroianu J, Radu A, Blobel G (1997): Karyopherin β2 mediates nuclear import of a mRNA binding protein. Proc Natl Acad Sci USA 94:5055-5060.
11. Brown K, Gerstberger S, Carlson L, Franzoso G, Siebenlist U (1995): Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation. Science 267:1485-1488.
12. Carey KL, Richards SA, Lounsbury KM, Macara IG (1996): Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the RanTC4 GTPase mediates an essential function independent of nuclear protein import. J Cell Biol 133:985-996.
13. Chi NC, Adam EJH, Visser GD, Adam SA (1996): RanBP1 stabilizes the interaction of Ran with p97 in nuclear protein import. J Cell Biol 135:559-569.
14. Clarkson WD, Kent HM, Stewart M (1996): Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p. J Mol Biol 263:517-524.
15. Conti E, Uy M, Leighton L, Blobel G, Kuriyan J (1998): Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α. Cell 94:193-204.
16. Corbett AH, Koepp DM, Schlenstedt G, Lee MS, Hooper AK, Silver PA (1995): Rna1p, a Ran/TC4 GTPase activating protein, is required for nuclear import. J Cell Biol 130:1017-1026.
17. Corbett AH, Silver PA (1996): The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo. J Biol Chem 271:18477-18484.
18. Cordes VC, Reidenbach S, Rackwitz HR, Franke WW (1997): Identification of protein p270/Tpr as a constitutive component of the nuclear pore complex-attached intranuclear filaments. J Cell Biol 136:515-529.
19. Coutavas E, Ren M, Oppenheim JD, D'Eustachio P, Rush MG (1993): Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. Nature 366:585-587.
20. Dasso M (1993): RCC1 in cell cycle: the regulator of chromosome condensation takes on new roles. Trends Biochem Sci 18:96-101.
21. Delphin C, Guan T, Melchior F, Gerace L (1997): RanGTP targets p97 to RanBP2, a filamentous protein localized at the cytoplasmic periphery of the nuclear pore complex. Mol Biol Cell 8:2379-2390.
22. DiDonato JA, Hayakawa M, Rothwarf D, Zandi E, Karin M (1997): A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 388:548-554.
23. Dingwall C, Laskey RA (1991): Nuclear targeting sequences - a consensus? Trends Biochem Sci 16:478-481.
24. Doye V, Hurt E (1997): From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9:401-411.
25. Drivas GT, Shih A, Coutavas E, Rush MG, D'Eustachio P (1990): Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line. Mol Cell Biol 10:1793-1798.
26. Englmeier L, Olivo J-C, Mattaj IW (1999): Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis. Curr Biol 9:30-41.
27. Fahrenkrog B, Hurt EC, Aebi U, Pante N (1998): Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143:577-588.
28. Feldherr CM, Kallenbach E, Schultz N (1984): Movement of a karyophilic protein through the nuclear pores of oocytes. Cell Biol Dev (6):2216-2222.
29. Feldherr C, Akin D (1991): Signal-mediated nuclear transport in proliferating and growth-arrested BALB/c 3T3 cells. Cell Biol 115(4):933-999.
30. Feldherr CM, Akin D (1993): Regulation of nuclear transport in proliferating and quiescent cells. Exp Cell Res 205(1):179-186.
31. Feldherr CM, Akin D (1994): Role of nuclear trafficking in regulating cellular activity. Int Rev Cytol 151:183-228.
32. Feldherr C, Akin D, Moore MS (1998): The nuclear import factor p10 regulates the functional size of the nuclear pore complex during oogenesis. J Cell Sci 111:1889-1896.
33. Feng W, Benko AL, Lee J-H, Stanford DR, Hopper AK (1999): Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution. J Cell Sci 112:339-347.
34. Ferrigno P, Posac F, Koepp D, Saito H and Silver PA (1998): Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin β homologs NMD5 and XPO1. EMBO J 17:5606-5614.
35. Fornerod M, van Deursen J, van Ball S, Murti KG, Fransen J, Grosveld G (1997a): The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO J 16:807-816.
36. Fornerod M, Ohno M, Yoshida M, Mattaj IW (1997b): CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 90:1051-1060.
37. Fridell RA, Truant R, Thorne L, Benson RE, Cullen BR (1997): Nuclear import of hnRNPA1 is mediated by a novel cellular cofactor related to karyopherin-beta. J Cell Sci 110:1325-1331.
38. Garcia-Bustos J, Heitman J, Hall MN (1991): Nuclear protein localization. Biochem Biophys Acta 1071:83-101.
39. Görlich D, Mattaj IW (1996): Nucleocytoplasmic transport. Science 271:1513-1517.
40. Görlich D, Vogel F, Mills AD, Hartmann E, Laskey RA (1995): Distinct functions for the two importin subunits in nuclear protein import. Nature 377:246-248.
41. Görlich D, Pante N, Kutay U, Aebi U, Bischoff FR (1996a): Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J 15:5584-5594.
42. Görlich D, Henklein P, Laskey RA, Hartmann E (1996b): A 41 amino acid motif in importin alpha confers binding to importin beta and hence transit into the nucleus. EMBO J 15:1810-1817.
43. Görlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S, Izaurralde E (1997): A novel class of RanGTP binding proteins. J Cell Biol 138:65-80.
44. Henderson BR, Percipalle P (1997): Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localization signal mediates specific binding to human importin-β. J Mol Biol 274:693-707.
45. Henkel T, Zabel U, van Zee K, Muller JM, Fanning E, Baeuerle PA (1992): Intramolecular masking of the nuclear location signal and dimerization domain in the precursor for the p50 NF-kappa B subunit. Cell 68:1121-1133.
46. Hood JK, Silver PA (1998): Cse1p is required for export of Srp1p/Importin-α from the nucleus in Saccharomyces cerevisiae. J Biol Chem 273:35142-35146.
47. Hopper AK, Traglia HM, Dunst RW (1990): The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus. J Cell Biol 111:309-321.
48. Huber J, Cronshagen U, Kadokura M, Marshallsay C, Wada T, Sekine M, Luhrmann R (1998): Snurportin 1, an m3G-cap-specific nuclear import receptor with a novel domain structure. EMBO J 17:4114-4126.
49. Imamoto N, Shimamoto T, Takao T, Tachibana T, Kose S, Matsubae M, Sekimoto T, Shimonishi Y, Yoneda Y (1995): In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import. EMBO J 14:3617-3626.
50. Imbert V, Rupec RA, Livolsi A, Pahl HL, Traenckner EMB et al. (1996): Tyrosine phosphorylation of I kappa B-alpha activates NF-kappa B without proteolytic degradation of I kappa B-alpha. Cell 86:787-798.
51. Iovine MK, Watkins JL, Wente SR (1995): The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor. J Cell Biol 131:1699-1713.
52. Iovine MK, Wente SR (1997): A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J Cell Biol 137:1-15.
53. Izaurralde E, Kutay U, Kobbe C, Mattaj IW, Görlich D (1997): The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J 16:6535-6547.
54. Izaurralde E, Adam S (1998): Transport of macromolecules between the nucleus and the cytoplasm. RNA 4:351-367.
55. Jakel S, Görlich D (1998): Importin β, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells. EMBO J 17:4491-4502.
56. Kadowaki T, Chen S, Hitomi M, Jacobs E, Kumagai C, Liang S, Schneiter R, Singleton D, Wisniewska J, Tartakoff AM (1994): Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J Cell Biol 126:649-659.
57. Kaffman A, Rank NM, O'Shea EK (1998a): Phosphorylation regulates association of the transcription factor Pho4 with its import receptor Pse1/Kap121. Genes Dev 12:2673-2683.
58. Kaffman A, Rank NM, O'Neill EM, Huang LS, O'Shea EA (1998b): The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396:482-486.
59. Kehlenbach RH, Dickmanns A, Gerace L (1998): Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT in vitro. J Cell Biol 141:863-874.
60. Kudo N, Khochbin S, Nishi K, Kitano K, Yanagida M, Yoshida M, Horinouchi S (1997): Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins. J Biol Chem 272:29742-29751.
61. Kutay U, Bischoff FR, Kosthka S, Kraft R, Görlich D (1997): Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 90:1061-1071.
62. Kutay U, Lipowsky G, Izaurralde E, Bischoff FR, Schwarzmeier P, Hartmann E, Görlich D (1998): Identification of a tRNA-specific nuclear export receptor. Mol Cell 1:1-20.
63. Lee MS, Henry M, Silver PA (1996): A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes Dev 10:1233-1246.
64. Lawler JF Jr, Yin M, Diehl AM, Roberts E, Chatterjee S (1998): Tumor necrosis factor-alpha stimulates the maturation of sterol regulatory element binding protein-1 in human hepatocytes through the action of neutral sphingomyelinase. J Biol Chem 273:5053-5059.
65. Lounsbury KM, Macara IG (1997): Ran-binding protein 1 (RanBP1) forms a ternary complex with Ran and karyopherin β and reduces Ran GTPase-activating protein (RanGAP) inhibition by karyopherin β. J Biol Chem 272:551-555.
66. Mahajan R, Delphin C, Guan T, Gerace L, Melchior F (1997): A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88:97-107.
67. Malik HS, Eickbush TH, Goldfarb DS (1997): Evolutionary specialization of the nuclear targeting apparatus. Proc Natl Acad Sci USA 94:13738-13742.
68. Marelli M, Aitchison JD, Wozniak RW (1998): Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p. J Cell Biol 143:1813-1830.
69. Mattaj IW, Englmeier L (1998): Nucleocytoplasmic transport: the soluble phase. Annu Rev Biochem 67:265-306.
70. Matunis MJ, Coutavas E, Blobel G (1997): A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein between cytosol and nuclear pore complex. J Cell Biol 135:1457-1470.
71. Melchior F, Paschal B, Evans J, Gerace L (1993): Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J Cell Biol 123:1649-1659.
72. Melchior F, Guan T, Yokoyama N, Nishimoto T, Gerace L (1995): GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import. J Cell Biol 131:571-581.
73. Moll T, Tebb G, Surana U, Robitsch H, Nasmyth K (1991): The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. Cell 66:743-758.
74. Moore MS, Blobel G (1992): The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors. Cell 69:939-950.
75. Moore MS, Blobel G (1993): The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature (London) 365:661-663.
76. Moore MS, Blobel G (1994): Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc Natl Acad Sci USA 91:10212-10216.
77. Moore JD, Yang J, Truant R, Kornbluth S (1999): Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1. J Cell Biol 144:213-224.
78. Moroianu J, Blobel G, Radu A (1995a): Previously identified protein of uncertain function is karyopherin α and together with karyopherin β docks import substrate at nuclear pore complexes. Proc Natl Acad Sci USA 92:2008-2011.
79. Moroianu J, Hijikata M, Blobel G, Radu A (1995b): Mammalian karyopherin α1β and α2β heterodimers: α1 or α2 subunits binds nuclear localization signal and β subunit interacts with peptide repeat-containing nucleoporins. Proc Natl Acad Sci USA 92:6532-6536.
80. Moroianu J, Blobel G, Radu A (1996): The binding site of karyopherin α for karyopherin β overlaps with a nuclear localization sequence. Proc Natl Acad Sci USA 93:6572-6576.
81. Moroianu J, Blobel G, Radu A (1997): RanGTP-mediated nuclear export of karyopherin α involves its interaction with the nucleoporin Nup153. Proc Natl Acad Sci USA 94:9699-9704.
82. Moroianu J (1998): Distinct nuclear import and export pathways mediated by members of the karyopherin β family. J Cell Biochem 70:231-239.
83. Nakielny S, Dreyfuss G (1997): Import and export of the nuclear protein import receptor transportin by a mechanism independent of GTP hydrolysis. Curr Biol 8:89-95.
84. Nehrbass U, Blobel G (1996): Role of the nuclear transport factor p10 in nuclear import. Science 272:120-122.
85. Neville M, Stutz F, Lee L, Davis LI, Rosbash M (1997): Evidence that the importin-beta family member Crm1p bridges the interaction between rev and the nuclear pore complex during nuclear export. Curr Biol 7:767-775.
86. Nigg EA (1997): Nucleocytoplasmic transport: signals, mechanisms and regulation. Nature 386:779-787.
87. Nishi K, Yoshida M, Fujiwara D, Nishikawa M, Horinouchi S, Beppu T(1994): Leptomycin B targets a regulatory cascade of CRM1, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression. J Biol Chem 269:6320-6324.
88. O'Neill RE, Palese P (1995): NPI-1, the human homolog of SRP-1, interacts with influenza virus nucleoprotein. Virol 206:116-125.
89. O'Neill RE, Jaskunas R, Blobel G, Palese P, Moroianu J (1995): Nuclear import of influenza virus RNA can be mediated by viral nucleoprotein and transport factors required for protein import. J Biol Chem 270:22701-22704.
90. Ohno M, Fornerod M, Mattaj IW (1998): Nucleocytoplasmic transport: the last 200 nanometers. Cell 92:327-336.
91. Ohtsubo M, Okazaki H, Nishimoto T (1989): The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and binds to DNA. J Cell Biol 109:1389-1397.
92. Ossareh-Nazari B, Bachelerie F, Dargemont C (1997): Evidence for a role of CRM1 in signal-mediated nuclear protein export. Science 278:141-144.
93. Palacios I, Weis K, Klebe C, Dingwall C (1996): Ran/TC4 mutants identify a common requirement for snRNP and protein import into the nucleus. J Cell Biol 133:485-494.
94. Palacios I, Hetzer M, Adam, SA, Mattaj IW (1997): Nuclear import of U snRNPs requires importin β. EMBO J 16:6783-6792.
95. Palombella VJ, Rando OJ, Goldberg AL, Maniatis T (1994): The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B. Cell 78:773-785.
96. Palmeri D, Malim MH (1999): Importin β can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin α. Mol Cell Biol 19:1218-1225.
97. Pante N, Bastos R, McMorrow I, Burke B, Aebi U (1994): Interactions and 3-D localization of a group of nuclear pore complex proteins. J Cell Biol 126:603-617.
98. Paschal BM, Gerace L (1996): Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J Cell Biol 129:925-937.
99. Peifer M, Berg S, Reynolds AB (1994): A repeating amino acid motif shared by proteins with diverse cellular roles. Cell 76:789-791.
100. Pemberton LF, Rosenblum JS, Blobel G (1997): A distinct and parallel pathway for the nuclear import of an mRNA-binding protein. J Cell Biol 139:1645-1653.
101. Pemberton LF, Blobel G, Rosenblum JS (1998): Transport routes through the nuclear pore complex. Curr Opinion Cell Biol 10:392-399.
102. Pollard VW, Michael WM, Nakielny S, Siomi MC, Wang F, Dreyfuss G (1996): A novel receptor-mediated nuclear protein import pathway. Cell 86:985-994.
103. Radu A, Blobel G, Moore MS (1995a): Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc Natl Acad Sci USA 92:1769-1773.
104. Radu A, Moore MS, Blobel G (1995b): The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell 81:1-8.
105. Rexach M, Blobel G (1995): Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83:683-692.
106. Ribbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1998a): NTF2 mediates nuclear import of Ran. EMBO J 16:6587-6598.
107. Ribbeck K, Kutay U, Paraskeva E, Görlich D (1999): The translocation of transportin-cargo complexes through nuclear pores is independent of both Ran and energy. Curr Biol 9:47-50.
108. Richards SA, Lounsbury KM, Macara IG (1995): The C-terminus of the nuclear Ran/TC4 GTPase stabilizes the GDP bound state and mediates interactions with RCC1, RanGAP and HTF9A/RanBP1. J Biol Chem 270: 14405-14411.
109. Richards SA, Lounsbury KM, Carey KL, Macara IG (1996): A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1. J Cell Biol 134:1157-1168.
110. Richards SA, Carey KL, Macara IG (1997): Requirement of guanosine triphosphate-bound Ran for signal-mediated nuclear protein export. Science 276:1842-1844.
111. Rosenblum JS, Pemberton LF, Blobel G (1997): A nuclear import pathway for a protein involved in tRNA maturation. J Cell Biol 139:1655-1661.
112. Rosenblum JS, Pemberton LF, Bonifaci N, Blobel G (1998): Nuclear import and the evolution of a multifunctional RNA-binding protein. J Cell Biol 143:887-899.
113. Rout MP, Wente SR (1994): Pores for thought: nuclear pore complex proteins. Trends Biochem Sci 4:357-365.
114. Rout MP, Blobel G, Aitchison JD (1997): A distinct nuclear import pathway used by ribosomal proteins. Cell 89:715-725.
115. Saitoh H, Cooke CA, Burgess WH, Earnshaw WC, Dasso M (1996): Direct and indirect association of the small GTPase RAN with nuclear pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts. Mol Biol Cell 7:1319-1334.
116. Saitoh H, Pu R, Cavenagh M, Dasso M (1997): RanBP2 associates with Ubc9p and a modified form of RanGAP1. Proc Natl Acad Sci USA 94:3736-3741.
117. Schlenstedt G, Saavedra C, Loeb J, Cole C, Silver P (1995): The GTP-bound form of yeast Ran/TC4 homologues blocks nuclear protein import and appearance of poly-(A)+ RNA in the cytoplasm. Proc Natl Acad Sci USA 92:225-229.
118. Schlenstedt G, Smirnova E, Deane R, Solsbacher J, Kutay U, Görlich D, Ponstingl H, Bischoff FR (1997): Yrb4p, a yeast Ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus. EMBO J 16:6237-6249.
119. Schwoebel ED, Talcott B, Cushman I, Moore MS (1998): Ran-dependent signal-mediated nuclear import does not require GTP hydrolysis by Ran. J Biol Chem 273:35170-35175.
120. Shah S, Tugendreich S, Forbes D (1998): Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr. J Cell Biol 141:31-49.
121. Shah S, Forbes DJ (1998): Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors. Curr Biol 8:1376-1386.
122. Shen EC, Henry MF, Weiss VH, Valentini SR, Silver PA, Lee MS (1998): Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev 12:679-691.
123. Shibasaki F, Price ER, Milan D, McKeon F (1996): Role of kinases and the phosphatase calcineurin in the nuclear shuttling of transcription factor NF-AT4. Nature 382:370-373.
124. Seedorf M, Silver PA (1997): Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci USA 94:8590-8595.
125. Seedorf M, Damelin M, Kahana J, Taura T, Silver PA (1999): Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase.Mol. Cell Biol 19:1547-1557.
126. Siomi H, Dreyfuss G (1995): A nuclear localization domain in the hnRNP A1 protein. J Cell Biol 129:551-560.
127. Siomi MC, Eder PS, Kataoka N, Wan L, Liu Q, Dreyfuss G (1997): Transportin-mediated nuclear import of heterogenous nuclear RNP proteins. J Cell Biol 138:1181-1192.
128. Smith A, Brownawell A, Macara IG (1998): Nuclear import of Ran is mediated by the transport factor NTF2. Curr Biol 8:1403-1406.
129. Stade K, Ford CS, Guthrie C, Weis K (1997): Exportin 1 (Crmlp) is an essential nuclear export factor. Cell 90:1041-1050.
130. Stewart M, Kent HM, McCoy AJ (1998): Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and GDP-bound form of the Ras-family GTPase Ran. J Mol Biol 277:635-646.
131. Stoffler D, Fahrenkrog B, Aebi U (1999a): The nuclear pore complex: from molecular architecture to functional dynamics. Curr Opin Cell Biol, in press.
132. Stoffler D, Goldie KN, Feja B, Aebi U (1999a): Calcium-mediated structural changes of native nuclear pore complexes monitored by time-lapse atomic force microscopy. J Mol Biol, in press.
133. Sukegawa J, Blobel G (1993): A nuclear pore complex protein that contains zinc fingers and faces the nucleoplasm. Cell 72:29-38.
134. Timmerman LA, Clipstone NA, Ho SN, Northrop JP, Crabtree GR (1996): Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression. Nature 383:837-840.
135. Traenckner EBM, Pahl HL, Henkel T, Schmidt KN, Wilk S, Baeuerle PA (1995): Phosphorylation of human I kappa B-alpha on serines 32 and 36 controls I kappa B-alpha proteolysis and NF-kappa B activation in response to diverse stimuli. EMBO J 14:2876-2883.
136. Truant R, Cullen BR (1999): The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin β-dependent nuclear localization signals. Mol Cell Biol 19:1210-1217.
137. Wang P, Palese P, O'Neill RE (1997): The NPI-1/NPI-3 (karyopherin α) binding site on the influenza A virus nucleoprotein NP is a nonconventional nuclear localization signal. J Virol 71:1850-1856.
138. Weis K, Mattaj IW, Lamond AI (1995): Identification of hSRPα as a functional receptor for nuclear localization sequence. Science 268:1049-1053.
139. Weis K, Ryder U, Lamond AL (1996): The conserved amino terminal domain of hSRP1α is essential for nuclear protein import. EMBO J 15:1818-1825.
140. Weis K (1998): Importins and exportins: how to get in and out of the nucleus. Trends Biochem Sci 23:185-189.
141. Wolff B, Sanglier JJ, Wang Y (1997): Leptomycin B is an inhibitor of nuclear export: Inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) rev protein and rev-dependent mRNA. Chem Biol 4:139-147.
142. Wong DH, Corbett AH, Kent HM, Stewart M, Silver PA (1997): Interaction between the small GTPase Ran/GSP1p and Ntf2p is required for nuclear transport. Mol Cell Biol 17:3755-3767.
143. Wozniak RW, Rout MP, Aitchison JD (1998): Karyopherins and kissing cousins. Trends Cell Biol 8:184-188.
144. Wu J, Matunis MJ, Kraemer D, Blobel G, Coutavas E (1995): Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J Biol Chem 270:14209-14213.
145. Yaseen NR, Blobel G (1997): Cloning and characterization of human karyopherin β 3. Proc Natl Acad Sci USA 94:4451-4456.
146. Yoo CJ, Wolin SL (1997): The yeast La protein is required for the 3′ endonucleolytic cleavage that matures tRNA precursors. Cell 89:393-402.
147. Yano R, Oakes ML, Tabb MM, Nomura M (1994): Yeast Srp1p has homology to Armadillo/Placoglobin/β catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure. Proc Natl Acad Sci USA 91:6880-6884.
148. Yokoyama N, Hayashi N, Seki T, Pante N, Ohba T, Nishii K, Kuma T, Hayashida T, Miyata T, Aebi U et al. (1995): A giant nucleopore protein that binds Ran/TC4. Nature 376:184-188.
149. Zimowska G, Aris JP, Paddy MR (1997): A Drosophila Tpr protein homolog is localized both in the extrachromosomal channel network and to nuclear pore complexes. J Cell Sci 110:927-944.