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Because spotting was used instead of in situ synthesis, oligonucleotide solutions of x = 0 and y = 0 could be mixed together and immobilized in the NW quadrant. Similarly, mixtures of oligos x = 0 + y = I, x = 1 + y = 0 and x = 1 + y = 1 were spotted in the NE, SW, and SE quadrants, respectively.
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