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Volumn 682, Issue , 1998, Pages 206-228

Thermodynamics of Duplex Formation and Mismatch Discrimination on Photolithographically Synthesized Oligonucleotide Arrays

Author keywords

[No Author keywords available]

Indexed keywords


EID: 0348195767     PISSN: 00976156     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article
Times cited : (94)

References (46)
  • 1
    • 0346726134 scopus 로고    scopus 로고
    • GeneChip® is a U.S. registered trademark of Affymetrix, Inc.
    • GeneChip® is a U.S. registered trademark of Affymetrix, Inc.
  • 28
    • 0347356554 scopus 로고    scopus 로고
    • note
    • The apparently high discrimination ratio for the 18-mer site at 55°C and 500 nM target concentration is an artefact of the high bulk fluorescent background and low surface signals encountered under these conditions.
  • 29
    • 0346095513 scopus 로고    scopus 로고
    • note
    • The sequence of photodeprotection and chemical coupling at match probe sites and corresponding mismatch probe sites of length L differ at only two steps out of a total of 4L synthesis steps. The probe densities and length distributions are thus expected to be quite similar.
  • 31
    • 0347356553 scopus 로고    scopus 로고
    • note
    • Probes that are truncated at the 3′ end, formed if hydroxyl groups become exposed as the synthesis proceeds, cannot be detected with the HPLC analysis described in Figure 7. The proportion of truncated species may therefore be underestimated.
  • 34
    • 0346095512 scopus 로고    scopus 로고
    • note
    • The energetics of the intermediate length duplexes were estimated by linear interpolation between our measured values for the 10-mer:20-mer and 20-mer:20-mer duplexes.
  • 36
    • 0347986865 scopus 로고    scopus 로고
    • Personal communication
    • (b) Arkles, B. A.; Personal communication.
    • Arkles, B.A.1
  • 38
    • 0346095511 scopus 로고    scopus 로고
    • note
    • 18 introducing some degree of clustering with consequent reduction of average nearest neighbor distances. In practice, some additional clustering may take place due to chemical factors such as formation of domains. Average nearest neighbor distances computed under the assumption of a square lattice must therefore be regarded as an upper limit.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.