DNA solution of the maximal clique problem

Science

Volumn 278, Issue 5337, 1997, Pages 446-449

  Ouyang, Qi ^a   Kaplan, Peter D ^a   Liu, Shumao ^a,b   Libchaber, Albert ^a,b  

^a NEC LABORATORIES AMERICA   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ALGORITHM; ARTICLE; CALCULATION; COMPUTER; DNA DETERMINATION; MOLECULAR BIOLOGY; PRIORITY JOURNAL; PROBLEM SOLVING;

ALGORITHMS; BASE SEQUENCE; COMPUTING METHODOLOGIES; DNA; DNA RESTRICTION ENZYMES; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; OLIGODEOXYRIBONUCLEOTIDES; POLYMERASE CHAIN REACTION;

EID: 0030725521     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5337.446     Document Type: Article

References (21)

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13. 4, 0.8 mM dNTP, pH 8.8, at 25°C] to a volume of 40 μl were processed for 25 cycles (94°C for 30 s, 65°C for 30 s, and 72°C for 30 s).
14. The restriction enzyme digest operation was conducted following the protocol of New England Biolabs.
15. 6 (GAATTGGGTACCCTGGATCCCGCCC). The first primer was phosphorylated at the 5′ end with T4 polynucleotide kinase (New England Biolabs). (ii) The dsDNA of the PCR solution in step (i) was digested by λ exonuclease (Gibco-BRL) following the supplier's instructions. The DNA strand that contains a 5′ phosphate was thus digested, (iii) Mutagenetic M13 phage DNA (containing answer DNA) was synthesized using the ssDNA from step (ii) as primer and wild-type M13 phage DNA as template.
16. Transfection, phage purification, and DNA extraction were conducted following standard protocols (20). The DNA sequencing was done by the Rockefeller University Protein/DNA Technology Center.
17. 4) with 2 units of S1 nuclease at room temperature for 2 min.
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20. J. Sambrook, E. F. Fritsch, T. Maniatis, Molecular Cloning (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1987).
21. We thank D. Boneh, who proposed the maximal clique problem and the binary number-based algorithm for solving it, and D. Thaler, who suggested the use of POA to build the data pool.