A rapid method for disrupting genes in the escherichia coli genome

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 9, 1998, Pages 1826-1829

  Kato, Chinatsu ^a   Ohmiya, Ryusuke ^a   Mizuno, Takeshi ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

E. sherichia coli; Gene disruption; Genome analysis; Plasmid vector

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; ESCHERICHIA COLI; GENETIC PROCEDURES; GENETICS;

ESCHERICHIA COLI; GENES, BACTERIAL; GENETIC TECHNIQUES; GENOME, BACTERIAL;

EID: 0032153603     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.1826     Document Type: Article

References (12)

1. Doolittle, R. F., Microbial genomes opened up. Nature, 392, 339-342 (1998).
2. Blattner, F. R., Plunkett III. G., Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W,., Kirpatrick, H. A., Goeden, M. A., Rose, D. J., Maw, B., and Shao, Y., The complete genome sequence of Escherichia coli K-12. Science, 277, 1453-1474 (1997).
3. Jasin, M., and Schimmel, P., Deletions of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragment. J. Bacteriol., 159, 783-786 (1984).
4. Winans, S. C., Elledge, S. J., Kruger, J. H., and Walker, G. C., Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli. J. Bacteriol., 161, 1219-121 (1985).
5. Russell, C. B., Thaler, D. S., and Dahlquist, F. W., Chromosomal transformation of Escherichia coli recD strains with linearized plasmids. J. Bacteriol., 171, 2609-2613 (1989).
6. Murphy, K. N., Use of bacteriophage X recombination functions to promote gene replacement in Escherichia coli. J. Bacteriol., 180, 2063-2071 (1998).
7. Gutterson, N. I., and Koshland, D. E., Replacement and amplification of bacterial genes with sequences altered in vitro. Proc. Natl. Acad. Sei. UAS, 80, 4894-4898 (1983).
8. Saarilhti, H. T., and Palva, E. T., In vivo transfer of chromosomal mutation onto multicopy plasmids utilizing polA strains: cloning of an ompR2 mutation in Escherichia coli K-12. FEMS Microbiol. Lett., 26, 27-33 (1985).
9. Hamilton, C. M., Aldea, M., Washburn, K. B., Babitzke, P., and Kushner, S. R., New method for generating deletions and gene replacement in Escherichia coli. J. Bacteriol., 171, 4617-4622 (1989).
10. Kato, M., Aiba, H., and Mizuno, T., Molecular analysis by deletion and site directed mutagenesis of the cis-acting upstream sequence involved in activation of the ompF promoter in Escherichia coli. J. Biochem. (Tokyo), 105, 341-347 (1989).
11. Link, A. J., Phillips, D., and Church, G. M., Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol, 179, 6228-6237 (1997).
12. S. Slater and R. Maurer, Simple phage-based system for generating allele replacements in Escherichia coli. J. Bacteriol., 175, 4260-4262 (1993).