Phosphate feeding improves high-cell-concentration NSO myeloma culture performance for monoclonal antibody production

Biotechnology and Bioengineering

Volumn 69, Issue 5, 2000, Pages 566-576

  Dezengotita, Vivian M ^a,b   Miller, William M ^b   Aunins, John G ^a   Zhou, Weichang ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b Northwestern University   (United States)

Author keywords

Apoptosis; Cell metabolism; Fed batch culture; Glutamine synthetase; Lactate consumption; Monoclonal antibody; NSO cells; Phosphorus

Indexed keywords

AMINO ACIDS; BATCH CELL CULTURE; COMPOSITION EFFECTS; DNA; ENZYMES; LIPIDS; METABOLISM; MONOCLONAL ANTIBODIES; PHOSPHORUS;

APOPTOSIS; GLUTAMINE SYNTHETASE;

ANIMAL CELL CULTURE;

DNA; LYSINE; MONOCLONAL ANTIBODY; PHOSPHATE; PHOSPHOLIPID; RNA;

ANTIBODY PRODUCTION; ANTIBODY TITER; APOPTOSIS; ARTICLE; BIOPROCESS; CELL DENSITY; CELL GROWTH; CELL METABOLISM; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; HUMAN; HUMAN CELL; MAXIMUM ALLOWABLE CONCENTRATION; MYELOMA;

AMINO ACIDS; ANTIBODIES, MONOCLONAL; APOPTOSIS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; HUMANS; LACTIC ACID; MULTIPLE MYELOMA; PHOSPHATES; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0034609598     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20000905)69:5<566::AID-BIT11>3.0.CO;2-4     Document Type: Article

References (36)

1. High level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable marker
2. 7 cells per mL and improves baculovirus-expressed recombinant protein yields
3. Metabolic-acidosis is a potent stimulus for cellular inorganic-phosphate generation in uremia
4. Monoclonal antibody process development using medium concentrates
5. In pursuit of the optimal fed-batch process for monoclonal antibody production
6. Glucose and lactate metabolism in C6 glioma cells: Evidence for the preferential utilization of lactate for cell oxidative metabolism
7. Enhancement of monoclonal antibody yield by hybridoma fed-batch culture, resulting in extended maintenance of viable cell population
8. Feeding of nutrients delays apoptotic death in fed-batch cultures of NSO myeloma cells
9. Starvation-induced programmed cell death of hybridoma cells: Prevention by amino acid mixtures
10. Cell suicide in starving hybridoma culture: Survival-signal effect of some amino acids
11. Major metabolic changes accompany transfection and selection for high level expression of recombinant genes
12. An improved chemically defined culture medium for strain L mouse cells based on growth responses to graded levels of nutrients including iron and zinc ions
13. Human antithrombin III variant production from recombinant BHK cells in a fed-batch culture with on-line control of glucose and glutamine concentrations
14. Controlling mammalian cell metabolism in bioreactors
15. Overexpression of bcl-2, apoptosis suppressing gene: Prolonged viable culture period of hybridoma and enhanced antibody production
16. Adaptive fuzzy control of nutrients concentration in fed-batch culture of mammalian cells
17. Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells
18. NSO myeloma cell death: Influence of bcl-2 overexpression
19. Adaptive response of the archaeon Sulfolobus acidocaldarius BC65 to phosphate starvation
20. Production of engineered antibodies in myeloma and hybridoma cells - Enhancements in gene expression and process design
21. Optimization of a fed-batch process for production of a recombinant antibody
22. Application of hypoosmolar medium to fed-batch culture of hybridoma cells for improvement of culture longevity
23. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium
24. Phosphate limitation affects global gene-expression in Thiobacillus ferrooxidans
25. In hybridoma cultures, deprivation of any single amino acid leads to apoptotic death, which is suppressed by the expression of the bcl-2 gene
26. Essential amino acid deprivation of hybridoma cells results in the induction of high levels of apoptosis, which is suppressed by bcl-2 expression
27. Gene-expression in response to multiple nutrientstarvation conditions in Salmonella typhimurium
28. Great expectations: Biopharmaceutical industry expects success from new drug therapies and a full product development pipeline
29. High cell density and high monoclonal antibody production through medium design and rational control in a bioreactor
30. Fed-batch culture of recombinant NSO myeloma cells with high monoclonal antibody production
31. On-line characterization of a hybridoma cell culture process
32. Alteration of mammalian cell metabolism by dynamic nutrient feeding
33. High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding