Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography

Analytical Biochemistry

Volumn 275, Issue 1, 1999, Pages 122-124

  Diogo, M M ^a,b   Queiroz, J A ^b   Monteiro, G A ^a,c   Prazeres, D M F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b UNIVERSITY OF BEIRA INTERIOR   (Portugal)

^c UNIVERSIDADE LUSÓFONA DE HUMANIDADES E TECNOLOGIAS   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROPHOBICITY; LIQUID CHROMATOGRAPHY;

HYDROPHOBIC INTERACTION CHROMATOGRAPHY;

HYDROPHOBIC CHROMATOGRAPHY;

PLASMID VECTOR;

ANION EXCHANGE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DENATURATION; HYDROPHOBICITY; LIGAND BINDING; PRIORITY JOURNAL;

EID: 0033231382     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4297     Document Type: Article

References (5)

1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1979;1513-1523.
2. Sayers J. R., Evans D., Thomason J. B. Identification and erradication of a denatured DNA isolated during alkaline lysis-based plasmid purification procedures. Anal. Biochem. 241:1996;186-189.
3. Prazeres D. M. F., Schluep T., Cooney C. Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. J. Chromatogr. A. 806:1998;31-45.
4. Sundberg L., Porath J. Preparation of absorbents for biospecific affinity chromatography. I. Attachment of group-containing ligands to insoluble polymers by means of bifunctional oxiranes. J. Chromatogr. 90:1974;87-98.
5. Queiroz J. A., Garcia F. A. P., Cabral J. M. S. Hydrophobic interaction chromatography of Chromobacterium viscosum lipase. J. Chromatogr. A. 707:1995;137-142.