Monitoring of process streams in the large-scale production and purification of plasmid DNA for gene therapy applications

Pharmacy and Pharmacology Communications

Volumn 5, Issue 1, 1999, Pages 57-59

  Ferreira, G N M ^a   Cabral, J M S ^a   Prazeres, D M F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; LIPOPOLYSACCHARIDE; PLASMID DNA; PROTEIN; RNA;

ANION EXCHANGE CHROMATOGRAPHY; CONFERENCE PAPER; DNA PURIFICATION; GEL FILTRATION; GENE THERAPY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0032968972     PISSN: 14608081     EISSN: None     Source Type: Journal    
DOI: 10.1211/146080899128734163     Document Type: Conference Paper

References (5)

1. Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F. (1997) A comparison of gel filtration chromatographic supports for plasmid purification. Biotech. Tech. 11: 417-420
2. Ledley, F. D. (1995) Nonviral gene therapy: the promise of genes as pharmaceutical products. Hum. Gene Ther. 6: 1129-1144
3. Maniatis, T., Fritsch, E. F., Sambrook, J. (1982) Molecular Cloning: Laboratory Manual. Cold Spring Harbor, New York
4. Prazeres, D. M., Schluep, T., Cooney, C. (1998) Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. J. Chromatogr. A 806: 31-45
5. USFDA (1996) Addendum to the points to consider in human somatic cell and gene therapy. USFDA Center for Biologics Evaluation and Research