Intrinsic effects of solvent polarity on enzymic activation energies

Biotechnology and Bioengineering

Volumn 67, Issue 1, 2000, Pages 112-116

  Kim, Jungbae ^a   Clark, Douglas S ^b   Dordick, Jonathan S ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b University of California   (United States)

Author keywords

Intrinsic activation energies; Nonaqueous enzymology; Solvent polarity; Subtilisin catalysis

Indexed keywords

ACTIVATION ENERGY; CATALYSIS; ENZYMES; ESTERIFICATION; FREE ENERGY; ORGANIC SOLVENTS; SOLUBILITY;

SOLVENT POLARITY;

BIOTECHNOLOGY;

SUBTILISIN;

ARTICLE; CATALYSIS; CHEMICAL REACTION; ENERGY; ENZYME ACTIVATION; ESTERIFICATION; NONHUMAN; THERMODYNAMICS;

BACILLUS; BIOTECHNOLOGY; KINETICS; SOLUBILITY; SOLVENTS; SUBTILISINS; THERMODYNAMICS;

EID: 0034606636     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000105)67:1<112::AID-BIT13>3.0.CO;2-E     Document Type: Article

References (21)

1. Chen CS, Sih CJ. 1989. General aspects and optimization of enantioselective biocatalysis in organic solvents. Angew Chem, Int Ed Engl 28: 695-707.
2. Dordick JS. 1991. Biocatalysis for industry. New York: Plenum Publishing Co.
3. Dordick JS. 1992. Designing enzymes for use in organic solvents. Biotechnol Prog 8:259-267.
4. Fersht A. 1985. Enzyme structure and mechanism. New York: W. H. Freeman and Company.
5. Ingold CK. 1969. Structure and Mechanism in Organic Chemistry. 2nd edition. Ithaca, NY: Cornell University Press.
6. Klibanov AM. 1997. Why are enzymes less active in organic solvents than in water? Trends Biotechnol 15:97-101.
7. Kraut J. 1988. How do enzymes work? Science 242:533-540.
8. Kreevoy MM, Truhlar DG. 1986. In: Bernasconi DF, editor. Techniques of chemistry. New York: Wiley. Vol 6, p 13-95.
9. Michels PC, Dordick JS, Clark DS. 1997. Dipole Formation and Solvent Electrostriction in Subtilisin Catalysis. J Am Chem Soc 119: 9331-9335.
10. Oster G. 1946. The dielectric properties of liquid mixtures. J Am Chem Soc 68:2036-2041.
11. Reichardt C. 1979. Solvent effects in organic chemistry. Weinheim: Verlag Chemie.
12. Schmitke JL, Wescott CR, Klibanov AM. 1996. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents. J Am Chem Soc 118:3360-3365.
13. Smith RR, Canady WJ. 1992. Solvation effects upon the thermodynamic substrate activity; correlation with the kinetics of enzyme catalyzed reactions. I. Effects of added reagents such as methanol upon α-chymotrypsin. Biophys Chem 43:173-187.
14. Taylor JR. 1997. An introduction to error analysis: the study of uncertainties in physical measurements. Sausalito, CA: University Science Books.
15. Truhlar DG, Hase WL, Hynes JT. 1983. Current status of transition-state theory. J Phys Chem 87:2664-2682.
16. Wangikar PP, Graycar TP, Estell DA, Clark DS, Dordick JS. 1993. Protein and solvent engineering of subtilisin BPN' in nearly anhydrous organic media. J Am Chem Soc 115:12231-12237.
17. Wangikar PP, Rich JO, Clark DS, Dordick JS. 1995. Probing enzymic transition state hydrophobicities. Biochemistry 34:12302-12310.
18. Wangikar PP, Carmichael D, Clark DS, Dordick JS. 1996. Active-site titration of serine proteases in organic solvents. Biotechnol Bioeng 50:329-335.
19. Wescott CR, Klibanov AM. 1997. Thermodynamic analysis of solvent effect on substrate specificity of lyophilized enzymes suspended in organic media. Biotechnol Bioeng 56:340-344.
20. Xu ZF, Affleck R, Wangikar PP, Suzawa V, Dordick JS, Clark DS. 1994. Transition state stabilization of subtilisins in organic solvents. Biotechnol Bioeng 43:515-520.
21. Zaks A, Klibanov AM. 1988. Enzymatic catalysis in nonaqueous solvents. J Biol Chem 263:3194-3201.