Important parameters affecting efficiency of protein refolding by reversed micelles

Biotechnology Progress

Volumn 16, Issue 6, 2000, Pages 1079-1085

  Goto, M ^a   Hashimoto, Y ^a   Fujita, T A ^a   Ono, T ^a   Furusaki, S ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL INCLUSION; EXTRACTION; OPERATIONAL PARAMETER; ORGANIC SOLVENT; PROTEIN AGGREGATION; PROTEIN DENATURATION; PROTEIN FOLDING; REVERSE MICELLE; RIBONUCLEASE A; TRIPEPTIDE;

ANIMALS; CATTLE; MICELLES; OXIDATION-REDUCTION; PROTEIN FOLDING; PROTEIN RENATURATION; RIBONUCLEASE, PANCREATIC; SOLUBILITY; WATER;

EID: 0034525743     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000073m     Document Type: Article

References (25)

1. Studies on the reduction and re-formation of protein disulfide bonds
2. Protein refolding at high concentration using size-exclusion chromatography
3. Oxidative renaturation of hen egg-white lysozyme. Folding vs Aggregation
4. Molecular and cellular aspects of thiol-disulfide exchange
5. Recovery of proteins and amino acids from reverse micelles by dehydration with molecular sieves
6. Oxidative renaturation of lysozyme at high concentrations
7. Protein refolding in reversed micelles
8. Protein refolding by reversed micelles utilizing solid - Liquid extraction technique
9. Mechanism of protein extraction from the solid state by water-in-oil microemulsions
10. Effect of AOT concentration of the recovery activity yield of cytochrome c through the reversed micellar extraction
11. Refolding of carbonic anhydrase assisted by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes
12. Catalysis of the oxidative folding of ribonucrease A by protein disulfide isomerase: Dependence of the rate on the composition of redox buffer
13. Effective renaturation of reduced lysozyme by gentle removal of urea
14. Effect of inclusion body contaminants on the oxidative renaturation of hen egg white lysozyme
15. Recovery of protein from water-in-oil microemulsions in highly concentrated form through dilution technique
16. The purification of eukaryotic polypeptides synthesized in Escherichia coli
17. The renaturation of reduced chymotrypsinogen A in guanidine HCl
18. Artificial chaperone-assisted refolding of carbonic anhydrase B
19. Artificial chaperone-assisted refolding of denatured-reduced lysozyme: Modulation of the competition between renaturation and aggregation
20. Regeneration of native secondary and tertiary structures by air oxidation of reduced ribonucrease
21. Micellar solubilization of enzymes in hydrocarbon solvent, enzymatic activity and spectroscopic properties of ribonucrease in n-octane
22. Effect of additives on refolding of a denatured protein
23. Oxidative refolding of denatured/reduced lysozyme utilizing the chaperone-like function of liposomes and immobilized liposome chromatography
24. Protein refolding at high concentration using detergent/phospholipid mixtures
25. Detergent, liposome, and micelle-assisted protein refolding