Protein refolding by reversed micelles utilizing solid-liquid extraction technique

Biotechnology and Bioengineering

Volumn 57, Issue 5, 1998, Pages 620-623

  Hashimoto, Yukihisa ^a   Ono, Tsutomu ^a   Goto, Masahiro ^a   Hatton, T Alan ^b  

^a KYUSHU UNIVERSITY   (Japan)

^b USA   (United States)

Author keywords

DNA; Protein refolding; Reversed micelles; RNase A; Solid liquid extraction

Indexed keywords

CATALYST ACTIVITY; DNA; EXTRACTION; GENETIC ENGINEERING; MICELLES; PRECIPITATION (CHEMICAL); REDOX REACTIONS; SODIUM COMPOUNDS; SOLUBILITY;

PROTEIN REFOLDING; SOLID LIQUID EXTRACTION;

ENZYME KINETICS;

DOCUSATE SODIUM; GLUTATHIONE; GLUTATHIONE DISULFIDE; RIBONUCLEASE A;

ARTICLE; LIQUID LIQUID EXTRACTION; MICELLE; PROTEIN DENATURATION; PROTEIN FOLDING; SOLID PHASE EXTRACTION; SOLUBILIZATION;

ACETONE; BIOCHEMISTRY; MICELLES; PRECIPITATION; PROTEIN DENATURATION; PROTEIN FOLDING; RIBONUCLEASE, PANCREATIC; SOLUBILITY;

EID: 0032485469     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980305)57:5<620::AID-BIT14>3.0.CO;2-F     Document Type: Article

References (7)

1. Anfinsen, C. B., Haber, E. 1961. Studies on the reduction and re-formation of protein disulfide bonds. J. Biol. Chem. 236: 1361-1363.
2. Batas, B., Chaundhuri, J. B. 1996. Protein refolding at high concentration using size-exclusion chromatography. Biotechnol. Bioeng. 50: 16.
3. Hagen, A. J., Hatton, T. A., Wang, D. I. C. 1989a. Protein refolding in reversed micelles. Biotechnol. Bioeng. 236: 1353-1360.
4. Hagen, A. J., Hatton, T. A., Wang, D. I. C. 1989b. Protein refolding in reversed micelles: Interactions of the protein with micelle components. Biotechnol. Bioeng. 35: 966-975.
5. Marston, F. A. O. 1986. The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 240: 1.
6. White, F. H., Jr. 1961. Regeneration of native secondary and tertiary structures by air oxidation of reduced ribonuclease. J. Biol. Chem. 236; 1353-1360.
7. Wolf, R., Luisi, P. L. 1979. Micellar solubilization of enzymes in hydrocarbon solvent, enzymatic activity and spectroscopic properties of ribonuclease in n-octane. Biochem. Biophys. Res. Commun. 89(1): 209.