ERM-Merlin and EBP50 protein families in plasma membrane organization and function

Annual Review of Cell and Developmental Biology

Volumn 16, Issue , 2000, Pages 113-143

  Bretscher, A ^a   Chambers, D ^a   Nguyen, R ^a   Reczek, D ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

Endocytosis; FERM domain; Microfilaments; PDZ domain; Rho

Indexed keywords

EZRIN; F ACTIN; MEMBRANE PROTEIN; MOESIN; RADIXIN; RHO FACTOR;

AMINO ACID SEQUENCE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELL SURFACE; CONFORMATIONAL TRANSITION; ENDOCYTOSIS; MICROFILAMENT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; REVIEW; SIGNAL TRANSDUCTION;

ANIMALS; CARRIER PROTEINS; CELL MEMBRANE; CYTOSKELETAL PROTEINS; HUMANS; MEMBRANE PROTEINS; NEUROFIBROMIN 2; PHOSPHOPROTEINS; PROTEIN CONFORMATION; SODIUM-HYDROGEN ANTIPORTER;

EID: 0034524664     PISSN: 10810706     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.cellbio.16.1.113     Document Type: Review

References (184)

1. Cytological transformations associated with parietal cell stimulation: Critical steps in the activation cascade
2. High levels of ezrin expressed by human pancreatic adenocarcinoma cell lines with high metastatic potential
3. Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker
4. Subcellular localization of moesin in dynamic filopodia, retraction fibers, and other structures involved in substrate exploration, attachment, and cell-cell contacts
5. Disruption of dynamic cell surface architecture of NIH3T3 fibroblasts by the N-terminal domains of moesin and ezrin: In vivo imaging with GFP fusion proteins
6. Radixin is a component of hepatocyte microvilli in situ
7. Glycophorin is linked by band 4.1 protein to the human erythrocyte membrane skeleton
8. Characterization of moesin in the sea urchin Lytechinus variegatus: Redistribution to the plasma membrane following fertilization is inhibited by cytochalasin B
9. Crystal structure of the vinculin tail suggests a pathway for activation
10. Ezrin is concentrated in the apical microvilli of a wide variety of epithelial cells whereas moesin is found primarily in endothelial cells
11. Ezrin oligomers are major cytoskeletal components of placental microvilli: A proposal for their involvement in cortical morphogenesis
12. Mutations in transcript isoforms of the neurofibromatosis 2 gene in multiple human tumour types
13. High frequency of inactivating mutations in the neurofibromatosis type 2 gene (NF2) in primary malignant mesotheliomas
14. Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney
15. Ezrin promotes morphogenesis of apical microvilli and basal infoldings in retinal pigment epithelium
16. Purification of an 80,000-dalton protein that is a component of the isolated microvillus cytoskeleton, and its localization in nonmuscle cells
17. Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A-431 cells by epidermal growth factor
18. Regulation of cortical structure by the ezrin-radixin-moesin protein family
19. Soluble ezrin purified from placenta exists as stable monomers and elongated dimers with masked C-terminal ezrin-radixin-moesin association domains
20. Ezrin: A protein requiting conformational activation to link microfilaments to the plasma membrane in the assembly of cell surface structures
21. A kinase-regulated PDZ-domain interaction controls endocytic sorting of the beta2-adrenergic receptor
22. Radixin is involved in lamellipodial stability during nerve growth cone motility
23. Dephosphorylation of ezrin as an early event in renal microvillar breakdown and anoxic injury
24. Cytoskeletal dissociation of ezrin during renal anoxia: Role in microvillar injury
25. Unopposed phosphatase action initiates ezrin dysfunction: A potential mechanism for anoxic injury
26. The FERM domain: A unique module involved in the linkage of cytoplasmic proteins to the membrane
27. Widespread but cell type-specific expression of the mouse neurofibromatosis type 2 gene
28. The mouse neurofibromatosis type 2 gene maps to chromosome 11
29. Similarities and differences between the effects of epidermal growth factor and Rous sarcoma virus
30. Ezrin is an effector of hepatocyte growth factor-mediated migration and morphogenesis in epithelial cells
31. Impaired interaction of naturally occurring mutant NF2 protein with actin-based cytoskeleton and membrane
32. Expression of the neurofibromatosis type 2 gene in human tissues
33. Normal development of mice and unimpaired cell adhesion/cell motility/actin-based cytoskeleton without compensatory up-regulation of ezrin or radixin in moesin gene knockout
34. Ezrin is a cyclic AMP-dependent protein kinase anchoring protein
35. Identification of ezrin as an 81-kDa tyrosinephosphorylated protein in T cells
36. The ezrin-like family of tyrosine kinase substrates: Receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformation
37. Isolation of mutations in the Drosophila homologues of the human neurofibromatosis 2 and yeast CDC42 genes using a simple and efficient reverse-genetic method
38. Membrane and protein recycling associated with gastric HCl secretion
39. Pumps and pathways for gastric HCl secretion
40. Moesin, like ezrin, colocalizes with actin in the cortical cytoskeleton in cultured cells, but its expression is more variable
41. Radixin is a novel member of the band 4.1 family
42. Heterotypic and homotypic associations between ezrin and moesin, two putative membrane-cytoskeletal linking proteins
43. Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site
44. Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway
45. Regulation of vinculin binding to talin and actin by phosphatidyl- inositol-4-5-bisphosphate
46. Schwann cell hyperplasia and tumors in transgenic mice expressing a naturally occurring mutant NF2 protein
47. Interdomain interaction of merlin isoforms and its influence on intermolecular binding to NHE-RF
48. The merlin tumor suppressor localizes preferentially in membrane ruffles
49. Hibernation induces expression of moesin in intestinal epithelial cells
50. cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1
51. The protein-tyrosine kinase substrate, p81, is homologous to a chicken microvillar core protein
52. Homotypic and heterotypic interaction of the neurofibromatosis 2 tumor suppressor protein merlin and the ERM protein ezrin
53. Neurofibromatosis 2: Loss of merlin's protective spell
54. Merlin: The neurofibromatosis 2 tumor suppressor
55. + exchanger regulatory factor family of PDZ proteins
56. + exchange
57. G protein-coupled receptor kinase 6A phosphorylates the Na(+)/H(+) exchanger regulatory factor via a PDZ domain-mediated interaction
58. The secretion-stimulated 80K phosphoprotein of parietal cells is ezrin, and has properties of a membrane cytoskeletal linker in the induced apical microvilli
59. Immunological localization of an 80-kDa phosphoprotein to the apical membrane of gastric parietal cells
60. Molecular cloning and characterization of alternatively spliced transcripts of the mouse neurofibromatosis 2 gene
61. Specific release of membrane-bound annexin II and cortical cytoskeletal elements by sequestration of membrane cholesterol
62. Immunofluorescence detection of ezrin/radixin/moesin (ERM) proteins with their carboxyl-terminal threonine phosphorylated in cultured cells and tissues
63. Association of ezrin with intercellular adhesion molecule-1 and -2 (ICAM-1 and ICAM-2). Regulation by phosphatidylinositol 4,5-bisphosphate
64. ICAM-2 redistributed by ezrin as a target for killer cells
65. Molecular dissection of radixin: Distinct and interdependent functions of the amino- and carboxy-terminal domains
66. Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: Possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway
67. Protein phosphatase 2C inactivates F-actin binding of human platelet moesin
68. Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions
69. Replacement of threonine 558, a critical site of phosphorylation of moesin in vivo, with aspartate activates F-actin binding of moesin. Regulation by conformational change
70. Neurofibromatosis 2 antisense oligodeoxynucleotides induce reversible inhibition of schwannomin synthesis and cell adhesion in STS26T and T98G cells
71. Induction of tyrosine phosphorylation and translocation of ezrin by hepatocyte growth factor/scatter factor
72. An intramolecular association between the head and tail domains of vinculin modulates talin binding
73. The carboxy-terminal tail domain of vinculin contains a cryptic binding site for acidic phospholipids
74. F-actin binding site masked by the intramolecular association of vinculin head and tail domains
75. A conserved motif in the tail domain of vinculin mediates association with and insertion into acidic phospholipid bilayers
76. Analysis of the neurofibromatosis 2 gene in human breast and hepatocellular carcinomas
77. A highly expressed 81 kDa protein in immortalized mouse fibroblast: Its proliferative function and identity with ezrin
78. Active site-directed protein regulation
79. Impairment of cell adhesion by expression of the mutant neurofibromatosis type 2 (NF2) genes which lack exons in the ERM- homology domain
80. ERM (ezrin/radixin/moesin)-based molecular mechanism of microvillar breakdown at an early stage of apoptosis
81. Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin
82. + exchanger NHE3 isoform is regulated by the phosphafidylinositol 3-kinase pathway
83. + exchanger NHE3 activity by cAMP-dependent protein kinase, phosphorylation-dependent and -independent mechanisms
84. Essential functions of ezrin in maintenance of cell shape and lamellipodial extension in normal and transformed fibroblasts
85. The role of NHERF and E3KARP in the cAMP-mediated inhibition of NHE3
86. A heparin-binding protein involved in inhibition of smooth-muscle cell proliferation
87. Moesin: A member of the protein 4.1-talin-ezrin family of proteins
88. Identification and functional analysis of the ezrin-binding site in the hyaluronan receptor, CD44
89. Ezrin becomes restricted to outer cells following asymmetrical division in the preimplantation mouse embryo
90. Rho- and rac-dependent assembly of focal adhesion complexes and actin filaments in permeabilized fibroblasts: An essential role for ezrin/radixin/moesin proteins
91. Expression level, subcellular distribution and Rho-GDI binding affinity of merlin in comparison with ezrin/radixin/moesin proteins
92. Interdomain interactions of radixin in vitro
93. Genomic structure of the human ezrin gene
94. ERM proteins in cell adhesion and membrane dynamics
95. Identification of the protein 4.1 binding interface on glycophorin C and p55, a homologue of the Drosophila discs-large tumor suppressor protein
96. In vitro binding studies suggest a membrane-associated complex between erythroid p55, protein 4.1, and glycophorin C
97. Ezrin NH2-terminal domain inhibits the cell extension activity of the COOH-terminal domain
98. Three determinants in ezrin are responsible for cell extension activity
99. Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
100. Activation of ERM proteins in vivo by rho involves phosphatidyl- inositol 4-phosphate 5-kinase and not ROCK kinases
101. Distinct cellular and subcellular patterns of expression imply distinct functions for the Drosophila homologues of moesin and the neurofibromatosis 2 tumor suppressor, merlin
102. Mice heterozygous for a mutation at the Nf2 tumor suppressor locus develop a range of highly metastatic tumors
103. The Nf2 tumor suppressor gene product is essential for extraembryonic development immediately prior to gastrulation
104. Induction of filopodium formation by a WASP-related actin- depolymerizing protein N-WASP
105. Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50
106. A PDZ-interacting domain in CFTR is an apical membrane polarization signal
107. NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins
108. Phosphorylation of threonine 558 in the carboxyl-terminal actin-binding domain of moesin by thrombin activation of human platelets
109. Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides
110. Identification of a phosphatidylinositol-4,5-bisphosphate-binding domain in the N-terminal region of ezrin
111. Phosphorylation of moesin by rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures
112. Suppression of radixin and moesin alters growth cone morphology, motility, and process formation in primary cultured neurons
113. r 75,000 plasma membrane protein of human choriocarcinoma cells
114. Cytovillin and other microvillar proteins of human choriocarcinoma cells
115. r 75,000 plasma membrane protein, cytovillin, into newly formed microvilli in herpes simplex and Semliki Forest virus infected human embryonal fibroblasts
116. Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin-binding tail domain
117. Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes
118. Protein kinase C-theta phosphorylation of moesin in the actin-binding sequence
119. The neurofibromatosis 2 (NF2) tumor suppressor gene encodes multiple alternatively spliced transcripts
120. Identification of EBP50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family
121. The carboxyl-terminal region of EBP50 binds to a site in the amino-terminal domain of ezrin that is masked in the dormant molecule
122. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
123. Alteration in a new gene encoding a putative membrane-organizing protein causes neuro-fibromatosis type 2
124. Genetic linkage of bilateral acoustic neurofibromatosis to a DNA marker on chromosome 22
125. A dual involvement of the amino-terminal domain of ezrin in F- and G-actin binding
126. Neurofibromatosis 2 gene in human colorectal cancer
127. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat
128. Neurofibromatosis 2 tumor suppressor protein colocalizes with ezrin and CD44 and associates with actin-containing cytoskeleton
129. A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites
130. Radixin, a barbed end-capping actin-modulating protein, is concentrated at the cleavage furrow during cytokinesis
131. Erratum
132. Differential expression of the microspike-associated protein moesin in human tissues
133. Neurofibromatosis 2 tumour suppressor schwannomin interacts with betaII-spectrin
134. Common pathogenetic mechanism for three tumor types in bilateral acoustic neurofibromatosis
135. Moesin interacts with the cytoplasmic region of intercellular adhesion molecule-3 and is redistributed to the uropod of T lymphocytes during cell polarization
136. CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell contacts
137. RhoA-dependent phosphorylation and relocalization of ERM proteins into apical membrane/actin protrusions in fibroblasts
138. Regulation of the neurofibromatosis type 2 tumor suppressor protein, merlin, by adhesion and growth arrest stimuli
139. Moesin, the major ERM protein of lymphocytes and platelets, differs from ezrin in its insensitivity to calpain
140. A dihydrophobic Leu-Val motif regulates the basolateral localization of CD44 in polarized Madin-Darby canine kidney epithelial cells
141. Interdomain binding mediates tumor growth suppression by the NF2 gene product
142. An apical PDZ protein anchors the cysticfibrosis transmembrane conductance regulator to the cytoskeleton
143. C-terminal threonine phosphorylation activates ERM proteins to link the cell's cortical lipid bilayer to the cytoskeleton
144. Expression of NF2-encoded merlin and related ERM family proteins in the human central nervous system
145. r 75,000 polypeptide defined by antibodies to a synthetic peptide based on a cloned human endogenous retroviral DNA sequence
146. Interaction of radixin with Rho small G protein GDP/GTP exchange protein Db1
147. Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein
148. Detection of cellular proteins that interact with the NF2 tumor suppressor gene product
149. Loss of merlin-p85 protein complex in NF2-related tumors
150. Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members
151. Ligation of CD4 surface antigen induces rapid tyrosine phosphorylation of the cytoskeletal protein ezrin
152. An anti-Ras function of neurofibromatosis type 2 gene product (NF2/Merlin)
153. A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor
154. A new 82-kD barbed end-capping protein (radixin) localized in the cell-to-cell adherens junction: Purification and characterization
155. ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons
156. Cortical actin organization: Lessons from ERM (Ezrin/Radixin/Moesin) proteins
157. ERM (ezrin/radixin/moesin) family: From cytoskeleton to signal transduction
158. ERM proteins: Head-to-tail regulation of actin-plasma membrane interaction
159. Structure-function relationships in the ezrin family and the effect of tumor-associated point mutations in neurofibromatosis 2 protein
160. Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family
161. r 75,000 protein. cDNA sequence, prokaryotic expression, and chromosomal localization
162. Characterization of an 80-kDa phosphoprotein involved in parietal cell stimulation
163. The ezrin protein family: Membrane-cytoskeleton interactions and disease associations
164. Peptide binding consensus of the NHE-RF-PDZ1 domain matches the C-terminal sequence of cystic fibrosis transmembrane conductance regulator (CFTR)
165. + exchanger and its regulation by cAMP-dependent protein kinase
166. + exchanger
167. + exchanger in rabbit kidney brush- border membranes
168. Regulation of renal Na(+)-H+ exchanger by cAMP-dependent protein kinase
169. + exchanger requires a dissociable phosphoprotein cofactor
170. Structure-function of recombinant Na/H exchanger regulatory factor (NHE- RF)
171. Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+)-H+ exchanger
172. Structure and localization on the X chromosome of the gene coding for the human filopodial protein moesin (MSN)
173. Molecular cloning, cDNA sequence, and chromosomal assignment of the human radixin gene and two dispersed pseudogenes
174. Merlin differentially associates with the microtubule and actin cytoskeleton
175. Analysis of molecular domains of epitope-tagged merlin isoforms in COS-7 cells and primary rat Schwann cells
176. Crystal structures of c-Src reveal features of its autoinhibitory mechanism
177. Polarized distribution of actin isoforms in gastric parietal cells
178. Biochemical characterization of ezrin-actin interaction
179. Ezrin-calpain I interactions in gastric parietal cells
180. Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2
181. Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated ERM proteins
182. + exchanger NHE3 and the cytoskeletal protein ezrin
183. + exchanger, NHE3, requires an associated regulatory protein
184. cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor