Function of transcription cleavage factors GreA and GreB at a regulatory pause site

Molecular Cell

Volumn 6, Issue 6, 2000, Pages 1275-1285

  Marr, Michael T ^a   Roberts, Jeffrey W ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR; ELONGATION FACTOR; GALACTOKINASE; RNA; RNA POLYMERASE; TRANSCRIPTION FACTOR;

ARTICLE; ENZYME ASSAY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE MUTATION; GENETIC TRANSCRIPTION; NONHUMAN; RNA CLEAVAGE; TRANSCRIPTION REGULATION;

ARCHAEA; BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 0034507953     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)00126-X     Document Type: Article

References (52)

1. RNA cleavage and chain elongation by Escherichia coli DNA-dependent RNA polymerase in a binary enzyme-RNA complex
2. RNA chain elongation by Escherichia coli RNA polymerase. Factors affecting the stability of elongating ternary complexes
3. Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals
4. Purification and assay of Escherichia coli transcript cleavage factors GreA and GreB
5. GreA protein: A transcription elongation factor from Escherichia coli
6. Transcript cleavage factors from E. coli
7. Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoter
8. Structure of a transcribing T7 RNA polymerase initiation complex
9. Multiple RNA polymerase conformations and GreA: Control of the fidelity of transcription
10. GreA-induced transcript cleavage in transcription complexes containing Escherichia coli RNA polymerase is controlled by multiple factors, including nascent transcript location and structure
11. Sequences required for anti-termination by phage 82 Q protein
12. Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site
13. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
14. Use of mono Q high resolution ion exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase
15. Transcription factor S, a cleavage induction factor of the archaeal RNA polymerase
16. Escherichia coli transcript cleavage factors GreA and GreB stimulate promoter escape and gene expression in vivo and in vitro
17. Global transposon mutagenesis and a minimal Mycoplasma genome
18. The RNA polymerase II ternary complex cleaves the nascent transcript in a 3′-5′ direction in the presence of elongation factor SII
19. Structure of transcription elongation complexes in vivo
20. Kinetics of RNA polymerase initiation and pausing at the lambda late gene promoter in vivo
21. A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein
22. RNA polymerase switches between inactivated and activated states by translocating back and forth along the DNA and the RNA
23. Transcriptional arrest: Escherichia coli RNA polymerase translocates backward, leaving the 3′ end of the RNA intact and extruded
24. RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes
25. Structural analysis of ternary complexes of Escherichia coli RNA polymerase. Individual complexes halted along different transcription units have distinct and unexpected biochemical properties
26. GreA-induced transcript cleavage is accompanied by reverse translocation to a different transcription complex conformation
27. Enhancing transcription through the Escherichia coli hemolysin operon, hlyCABD: RfaH and upstream JUMPStart DNA sequences function together via a postinitiation mechanism
28. Promoter associated pausing in promoter architecture and postinitiation transcription regulation
29. Mapping interactions of Escherichia coli GreB with RNA polymerase and ternary elongation complexes
30. On the mechanism of rifampicin inhibition of RNA synthesis
31. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase
32. Abortive initiation and long ribonucleic acid synthesis
33. Topology of the RNA polymerase active center probed by chimeric rifampicin-nucleotide compounds
34. The RNA-DNA hybrid maintains the register of transcription by preventing backtracking of RNA polymerase
35. Intrinsic transcript cleavage activity of RNA polymerase
36. Visualization of the binding site for the transcript cleavage factor GreB on Escherichia coli RNA poiymerase
37. P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II
38. Promoter proximal sequences modulate RNA polymerase II by a novel mechanism
39. Transcription elongation factor SII (TFIIS) enables RNA polymerase II to elongate through a block to transcription in a human gene in vitro
40. Function of a nontranscribed DNA strand site in transcription elongation
41. Function of E. coli RNA polymerase sigma factor sigma 70 in promoter-proximal pausing
42. Antitermination by bacteriophage lambda Q protein
43. The nucleotide sequence of greA, a suppressor gene that restores growth of an Escherichia coli RNA polymerase mutant at high temperature
44. A stressed intermediate in the formation of stably initiated RNA chains at the Escherichia coli lac UV5 promoter
45. Spontaneous cleavage of RNA in ternary complexes of Escherichia coli RNA polymerase and its significance for the mechanism of transcription
46. Basic mechanisms of transcript elongation and its regulation
47. Gene Q antiterminator proteins of Escherichia coli phages 82 and lambda suppress pausing by RNA polymerase at a rho-dependent terminator and at other sites
48. Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site
49. Mechanism of intrinsic transcription termination and antitermination
50. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution