Expression and regulation of RUNX2/Cbfa1 and osteoblast phenotypic markers during the growth and differentiation of human osteoblasts

Journal of Cellular Biochemistry

Volumn 80, Issue 3, 2001, Pages 424-440

  Prince, Michelle ^b   Banerjee, Chaitali ^b   Javed, Amjad ^b   Green, Jack ^b   Lian, Jane B ^a   Stein, Gary S ^b   Bodine, Peter V N ^b   Komm, Barry S ^b  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

^b WYETH AYERST RESEARCH   (United States)

Author keywords

17 estradiol; Dexamethasone; Osteoblast maturation; Transforming growth factor 1; Vitamin D3

Indexed keywords

CELL MARKER; COLECALCIFEROL; DEXAMETHASONE; ESTRADIOL; GROWTH FACTOR; RNA POLYMERASE II; STEROID; TRANSFORMING GROWTH FACTOR BETA1;

ARTICLE; CELL DIFFERENTIATION; CELL GROWTH; DNA BINDING; EMBRYO DEVELOPMENT; GEL MOBILITY SHIFT ASSAY; GENE CONTROL; GENE EXPRESSION REGULATION; HEMATOPOIESIS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MINERALIZATION; OSTEOBLAST; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; WESTERN BLOTTING;

EID: 0034482638     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-4644(20010301)80:3<424::AID-JCB160>3.0.CO;2-6     Document Type: Article

References (71)

1. AP-1 and vitamin D receptor (VDR) signaling pathways converge at the rat osteocalcin VDR element: Requirement for the internal activating protein-1 site for vitamin D-mediated trans-activation
2. The osteoblast lineage
3. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteocalcin gene
4. Runt homology domain proteins in osteoblast differentiation: AML-3/CBFA1 is a major component of a bone specific complex
5. Osteoprogenitor cells in cell populations derived from mouse and rat calvaria differ in their response to corticosterone, cortisol, and cortisone
6. Nucleotide sequences of complementary deoxyribonucleic acids for the pro alpha 1 chain of human type I procollagen. Statistical evaluation of structures that are conserved during evolution
7. Development and characterization of a conditionally transformed adult human osteoblastic cell line
8. Establishment and hormonal regulation of a conditionally-transformed preosteocytic cell line from adult human bone
9. Functional properties of a conditionally-phenotypic, estrogen-responsive, human osteoblast cell line
10. 3 promotes osteoblast differentiation and matrix mineralization of a conditionally transformed adult human osteoblast cell line
11. TGF beta alters growth and differentiation related gene expression in proliferating osteoblasts in vitro, preventing development of the mature bone phenotype
12. 3 on collagen type I, osteopontin, and osteocalcin gene expression in chicken osteoblasts
13. Clinical review 83: Mechanisms of glucocorticoid action in bone: Implications to glucocorticoid-induced osteoporosis
14. Isolation of the human gene for bone gla protein utilizing mouse and rat cDNA clones
15. Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone
16. Reduction in transforming growth factor beta receptor I expression and transcription factor CBFa1 on bone cells by glucocorticoid
17. Expression of bone matrix proteins during dexamethasone-induced mineralization of human bone marrow stromal cells
18. Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation
19. Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: Three-dimensional organization and protein composition
20. Activity of the osteocalcin promoter in skeletal sites of transgenic mice and during osteoblast differentiation in bone marrow-derived stromal cell cultures: Effects of age and sex
21. Potential role of Cbfa1, an essential transcriptional factor for osteoblast differentiation, in osteoclastogenesis: Regulation of mRNA expression of osteoclast differentiation factor (ODF)
22. Genomic organization, expression of the human CBFA1 gene, and evidence for an alternative splicing event affecting protein function
23. Variable hormone responsiveness of osteoblast populations isolated at different stages of embryogenesis and its relationship to the osteogenic lineage
24. Differentiation of human marrow stromal precursor cells: Bone morphogenetic protein-2 increases OSF2/CBFA1, enhances osteoblast commitment, and inhibits late adipocyte maturation
25. Differential cell surface expression of the STRO-1 and alkaline phosphatase antigens on discrete developmental stages in primary cultures of human bone cells
26. Stimulation of osteoprotegerin ligand and inhibition of osteoprotegerin production by glucocorticoids in human osteoblastic lineage cells: Potential paracrine mechanisms of glucocorticoid-induced osteoporosis
27. Transcriptional control of the tissue-specific, developmentally regulated osteocalcin gene requires a binding motif for the Msx family of homeodomain proteins
28. Maturational disturbance of chondrocytes in Cbfa1-deficient mice
29. CBFa(AML/PEBP2)-related elements in the TGF-beta type I receptor promoter and expression with osteoblast differentiation
30. Regulation of chondrocyte differentiation by Cbfa1
31. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts
32. Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor β1 in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not sufficient for osteoblast differentiation
33. Immortalization of virus-free human placental cells that express tissue-specific functions
34. AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization
35. Osteoblast biology
36. The cells of bone
37. Regulated expression of the bone-specific osteocalcin gene by vitamins and hormones
38. Subcellular partitioning of transcription factors during osteoblast differentiation: Developmental association of the AML/CBFα/PEBP2α-related transcription factor-NMP-2 with the nuclear matrix
39. The quantitative histochemistry of brain II. Enzyme measurements
40. Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures
41. New developments in the pathogenesis and treatment of steroid-induced osteoporosis
42. The tissue-specific nuclear matrix protein, NMP-2, is a member of the AML/CBF/PEBP2/runt domain transcription factor family: Interactions with the osteocalcin gene promoter
43. AML-2 is a potential target for transcriptional regulation by the t(8;21) and t(12;21) fusion proteins in acute leukemia
44. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
45. Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia
46. Identification of an OSF-2 binding site in the murine RANKL/OPGL gene promoter: A potential link between osteoblastogenesis and osteoclastogenesis
47. PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene
48. AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis
49. Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development
50. Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix
51. Pleiotropic effects of vitamin D on osteoblast gene expression are related to the proliferative and differentiated state of the bone cell phenotype: Dependency upon basal levels of gene expression, duration of exposure, and bone matrix competency in normal rat osteoblast cultures
52. Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene
53. Vertebrate mineralized matrix proteins: Structure and function
54. Human bone cells in vitro
55. Expression of the Runt domain-encoding PEBP2 alpha genes in T cells during thymic development
56. Glucocorticoids promote development of the osteoblast phenotype by selectively modulating expression of cell growth and differentiation associated genes
57. Multiple levels of steroid hormone-dependent control of osteocalcin during osteoblast differentiation: Glucocorticoid regulation of basal and vitamin D stimulated gene expression
58. A new transcription factor family associated with human leukemias
59. Mechanisms regulating osteoblast proliferation and differentiation
60. Proviral insertions induce the expression of bone-specific isoforms of PEBP2alphaA (CBFA1): Evidence for a new myc collaborating oncogene
61. Molecular analysis of the cDNA for human SPARC/osteonectin/BM-40: Sequence, expression, and localization of the gene to chromosome 5q31-q33
62. Crystal structure of the nuclear matrix targeting signal of the transcription factor AML-1/PEBP2αB/CBFα2
63. Two domains unique to osteoblast-specific transcription factor Osf2/Cbfa1 contribute to its transactivation function and its inability to heterodimerize with Cbf beta
64. Osteoprotegerin mRNA is expressed in primary human osteoblast-like cells: Down-regulation by glucocorticoids
65. Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis
66. Structure of the human liver/bone/kidney alkaline phosphatase gene
67. Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor
68. MAPK pathways activate and phosphorylate the osteoblast-specific transcription factor, Cbfa1
69. Identification of a nuclear matrix targeting signal in the leukemia and bone-related AML/CBFα transcription factors
70. Intranucleartargeting of AML/CBFα regulatory factors to nuclear matrix- associated transcriptional domains
71. 3 inhibits osteocalcin expression in mouse though an indirect mechanism