Functional properties of a conditionally phenotypic, estrogen- responsive, human osteoblast cell line

Journal of Cellular Biochemistry

Volumn 65, Issue 3, 1997, Pages 368-387

  Bodine, Peter V N ^a   Green, Jack ^b   Harris, Heather A ^a   Bhat, Ramesh A ^a   Stein, Gary S ^b   Lian, Jane B ^b   Komm, Barry S ^a  

^a Women's Health Research Institute   (United States)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

alkaline phosphatase; bone; clonal; dexamethasone; immortalized; interleukin 6; mineralization; osteocalcin; osteoporosis; parathyroid hormone; vitamin D3

Indexed keywords

ALKALINE PHOSPHATASE; CALCITRIOL; COLLAGEN TYPE 2; CYCLIC AMP; CYTOKINE; ESTRADIOL; ESTROGEN; ESTROGEN RECEPTOR; GROWTH FACTOR; INTERLEUKIN 6; MESSENGER RNA; OSTEOCALCIN; PARATHYROID HORMONE;

ARTICLE; BONE ATROPHY; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVITY; ESTROGEN RELEASE; HORMONE RESPONSIVE ELEMENT; HUMAN; HUMAN CELL; OSTEOBLAST; POSTMENOPAUSE OSTEOPOROSIS; PRIORITY JOURNAL; PROTEIN DNA BINDING;

AGED; ALKALINE PHOSPHATASE; ANTIGENS, POLYOMAVIRUS TRANSFORMING; CALCITRIOL; CELL DIVISION; CELL LINE; CELL LINE, TRANSFORMED; ESTRADIOL; FEMALE; GENE EXPRESSION; HUMANS; OSTEOBLASTS; OSTEOCALCIN; PHENOTYPE; POLYMERASE CHAIN REACTION; RECEPTORS, ESTRADIOL; TEMPERATURE;

ADENOVIRIDAE; MINK CELL FOCUS-FORMING VIRUS;

EID: 0030992469     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(19970601)65:3<368::AID-JCB7>3.0.CO;2-Q     Document Type: Article

References (75)

1. Bankson DD, Rifai N, Williams ME, Silverman LM, Gray TK (1989): Biochemical effects of 17β-estradiol on UMR106 cells. Bone Miner 6:55-63.
2. Bellows CG, Aubin JE, Heersche JNM (1987): Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro. Endocrinology 121:1985-1992.
3. Benz DJ, Haussler MR, Komm BS (1991): Estrogen binding and estrogenic responses in normal human osteoblast-like cells. J Bone Miner Res 6:531-541.
4. Bodine PVN, TrailSmith M, Komm BS (1996a): Development and characterization of a conditionally transformed adult human osteoblastic cell line. J Bone Miner Res 11:806-819.
5. Bodine PVN, Vernon SK, Komm BS (1996b): Establishment and hormonal regulation of a conditionally-transformed pre-osteocytic cell line from adult human bone. Endocrinology 137:4592-4604.
6. Bradford M (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
7. Chaudhary LR, Spelsberg TC, Riggs BL (1992): Production of various cytokines by normal human osteoblast-like cells in response to interleukin-1β and tumor necrosis factor-α: Lack of regulation by 17β-estradiol. Endocrinology 130:2528-2534.
8. Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli L (1994): Differentiation of human bone marrow osteogenic stromal cells in vitro: Induction of the osteoblast phenotype by dexamethasone. Endocrinology 134:277-286.
9. Chou JY (1989): Differentiated mammalian cell lines immortalized by temperature-sensitive tumor viruses. Mol Endocrinol 3:1511-1514.
10. Cissel DS, Murty M, Whipkey DL, Blaha JD, Graeber GM, Keeting PE (1996): Estrogen pretreatment increases arachidonic acid release by bradykinin stimulated normal human osteoblast-like cells. J Cell Biochem 60:260-270.
11. Clark G (1981): Miscellaneous stains. In Clark G (ed): "Staining Procedures." 4th Ed. Baltimore: Williams & Wilkins, pp 187.
12. Clover J, Gowen M (1994): Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone 15:585-591.
13. Colston KW, King RJB, Hayward J, Fraser DI, Horton MA, Stevenson JC, Arnett TR (1989): Estrogen receptors and human bone cells: Immunocytochemical studies. J Bone Miner Res 4:625-631.
14. r heat shock protein expression in osteoblasts. J Biol Chem 269:7869-7873.
15. Davis VL, Couse JF, Gray TK, Korach KS (1994): Correlation between low levels of estrogen receptors and estrogen responsiveness in two rat osteoblast-like cell lines. J Bone Miner Res 9:983-991.
16. Dempster DW, Lindsay R (1993): Pathogenesis of osteoporosis. Lancet 341:797-801.
17. Derynck R, Jarrett JA, Chen EY, Eaton DH, Bell JR, Assioan RK, Roberts AB, Sporn MB, Goeddel DV (1985): Human transforming growth factor-β complementary DNA sequence and expression in normal and transformed cells. Nature 316:701-705.
18. Eielson C, Kaplan D, Mitnick MA, Paliwal I, Insogna K (1994): Estrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells. Endocrinology 135:1639-1644.
19. Eriksen EF, Colvard DS, Berg NJ, Graham ML, Mann KG, Spelsberg TC, Riggs BL (1988): Evidence of estrogen receptors in normal human osteoblast-like cells. Science 241:84-86.
20. Ernst M, Rodan GA (1991): Estradiol regulation of insulin-like growth factor-I expression in osteoblastic cells: Evidence for transcriptional control. Mol Endocrinol 5:1081-1089.
21. Ernst M, Parker MG, Rodan GA (1991): Functional estrogen receptors in osteoblastic cells demonstrated by transfection with a reporter gene containing an estrogen response element. Mol Endocrinol 5:1597-1606.
22. Ernst M, Heath JK, Rodan GA (1989): Estradiol effects on proliferation, messenger ribonucleic acid for collagen and insulin-like growth factor-I, and parathyroid hormone-stimulated adenylate cyclase activity in osteoblastic cells from calvariae and long bones. Endocrinology 125:825-833.
23. Etienne MC, Fischel JL, Milano G, Formento P, Formento JL, Francoual M, Frenay M, Namer M (1990): Steroid receptors in human osteoblast-like cells. Eur J Cancer 26:807-810.
24. Fukayama S, Tashjian AH (1989): Direct modulation by estradiol of the response of human bone cells (SaOS-2) to human parathyroid hormone (PTH) and PTH-related protein. Endocrinology 124:397-401.
25. Gehron Robey P, Termine JD (1985): Human bone cells in vitro. Calcif Tissue Int 37:453-460.
26. Girasole G, Jilka RL, Passeri G, Boswell S, Boder G, Williams DC, Manolagas SC (1992): 17β-Estradiol inhibits interleukin-6 production by bone marrow-derived stromal cells and osteoblastic cells in vitro: A potential mechanism for the antiosteoporotic effect of estrogens. J Clin Invest 89:883-891.
27. Gray TK, Lipes B, Linkhart T, Mohan S, Baylink DJ (1989a): Transforming growth factor beta mediates the estrogen induced inhibition of UMR106 cell growth. Connective Tissue Res 20:23-32.
28. Gray TK, Mohan S, Linkhart TA, Baylink DJ (1989b): Estradiol stimulates in vitro the secretion of insulin-like growth factors by the clonal osteoblastic cell line, UMR106. Biochem Biophys Res Commun 158:407-412.
29. Gray TK, Flynn TC, Gray KM, Nabell LM (1987): 17β-Estradiol acts directly on the clonal osteoblastic cell line UMR106. Proc Natl Acad Sci USA 84:6267-6271.
30. Harris SA, Tau KR, Enger RJ, Toft DO, Riggs BL, Spelsberg TC (1995): Estrogen response in the hFOB1.19 human fetal osteoblastic cell line stably transfected with the human estrogen receptor gene. J Cell Biochem 59:193-201.
31. Harris SA, Subramaniam M, Riggs BL, Spelsberg TC (1992): Estrogen induces c-fos promoter activity in normal human osteoblast-like cells. J Bone Miner Res 7:S94.
32. Hayward-Lester A, Oefner PJ, Doris PA (1996): Rapid quantification of gene expression by competitive RT-PCR and ion-pair reversed-phase HPLC. BioTechniques 20:250-257.
33. Hayward-Lester A, Oefner PJ, Sabatini S, Doris PA (1995): Accurate and absolute quantitative measurement of gene expression by single-tube RT-PCR and HPLC. Genome Res 5:494-499.
34. Huo B, Dossing DA, Dimuzio MT (1995): Generation and characterization of a human osteosarcoma cell line stably transfected with the human estrogen receptor gene. J Bone Miner Res 10:769-781.
35. Ikegami A, Inoue S, Hosoi T, Kaneki M, Mizuno Y, Akedo Y, Ouchi Y, Orimo H (1994): Cell cycle-dependent expression of estrogen receptor and effect of estrogen on proliferation of synchronized human osteoblast-like osteosarcoma cells. Endocrinology 135:782-789.
36. Ikegami A, Inoue S, Hosoi T, Mizuno Y, Nakamura T, Ouchi Y, Orimo H (1993): Immunohistochemical detection and northern blot analysis of estrogen receptors in osteoblastic cells. J Bone Miner Res 8:1103-1109.
37. 3 in human osteosarcoma-derived osteoblast-like cells. Calcif Tissue Int 57:430-435.
38. Kaji H, Sugimoto T, Kanatani M, Nasu M, Chihara K (1996): Estrogen blocks parathyroid hormone (PTH)-stimulated osteoclast-like cell formation by selectively affecting PTH-responsive cyclic adenosine monophosphate pathway. Endocrinology 137:2217-2224.
39. Kaplan FS, Fallon MD, Boden SD, Schmidt R, Senior M, Haddad JG (1988): Estrogen receptors in bone in a patient with polyostotic fibrous dysplasia (McCune-Albright Syndrome). N Engl J Med 319:421-425.
40. Kasid A, Strobol JS, Huff K, Greene GL, Lippman ME (1984): A novel nuclear form of estradiol receptor in MCF-7 human breast cancer cells. Science 225:1162-1165.
41. Kassem M, Harris SA, Spelsberg TC, Riggs BL (1996): Estrogen inhibits interleukin-6 production and gene expression in a human osteoblastic cell line with high levels of estrogen receptors. J Bone Miner Res 11:193-199.
42. Keeting PE, Scott RE, Colvard DS, Anderson MA, Oursler MJ, Spelsberg TC, Riggs BL (1992): Development and characterization of a rapidly proliferating, well-differentiated cell line derived from normal adult human osteoblast-like cells transfected with SV40 large T antigen. J Bone Miner Res 7:127-136.
43. Klein-Hitpass L, Ryffel GU, Heitlinger E, Cato ACB (1988): A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with the estrogen receptor. Nucleic Acids Res 16:647-664.
44. Komm BS, Terpening CM, Benz DJ, Graeme KA, Gallegos A, Korc M, Greene GL, O'Malley BW, Haussler MR (1988): Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. Science 241:81-84.
45. Lajeunesse D (1994): Effect of 17β-estradiol on the human osteosarcoma cell line MG-63. Bone Miner 24:1-16.
46. Leboy PS, Beresford JN, Devlin C, Owen ME (1991): Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures. J Cell Physiol 146:370-378.
47. Lei K-J, Gluzman Y, Pan C-J, Chou JY (1992): Immortalization of virus-free human placental cells that express tissue-specific functions. Mol Endocrinol 6:703-712.
48. Liel Y, Kraus S, Levy J, Shany S (1992): Evidence that estrogens modulate activity and increase the number of 1,25-dihydroxyvitamin D receptors in osteoblast-like cells (ROS 17/2.8). Endocrinology 130:2597-2601.
49. Lowry OH, Roberts NR, Wu M, Hixen WS, Crawford D (1954): The quantitative histochemistry of brain. II. Enzyme measurements. J Biol Chem 207:13-19.
50. Mahonen A, Maenpaa PH (1994): Steroid hormone modulation of vitamin D receptor levels in human MG-63 osteosarcoma cells. Biochem Biophys Res Commun 205:1179-1186.
51. Majeska RJ, Ryaby JT, Einhorn TA (1994): Direct modulation of osteoblastic activity with estrogen. J Bone Joint Surg 76:713-721.
52. Manolagas SC, Jilka RL (1995): Bone marrow, cytokines, and bone remodeling: Emerging insights into the pathophysiology of osteoporosis. N Engl J Med 332:305-311.
53. Masuyama A, Ouchi Y, Sato T, Hosoi T, Nakamura T, Orimo H (1992): Characteristics of steroid receptors in cultured MC3T3-E1 osteoblastic cells and effect of steroid hormones on cell proliferation. Calcif Tissue Int 51:376-381.
54. Migliaccio S, Davis VL, Gibson MK, Gray TK, Korach KS (1992): Estrogens modulate the responsiveness of osteoblast-like cells (ROS 17/2.8) stably transfected with estrogen receptor. Endocrinology 130:2617-2624.
55. Monroe JJ, Tashjian AH (1996): Pre-treatment with 17β-estradiol attenuates basal-and PTH-stimulated membrane adenylate cyclase in human osteoblast-like SaOS-2 cells. Biochem Biophys Res Commun 225:320-325.
56. Oursler MJ, Cortese C, Keeting P, Anderson MA, Bonde SK, Riggs BL, Spelsberg TC (1991): Modulation of transforming growth factor-β production in normal human osteoblast-like cells by 17β-estradiol and parathyroid hormone. Endocrinology 129:3313-3320.
57. Pauli U, Chiu JF, DiTullio P, Kroeger P, Shalhoub V, Rowe T, Stein G, Stein J (1989): Specific interactions of histone H1 and a 45 kilodalton nuclear protein with a putative matrix attachment site in the distal promoter region of a cell-regulated human histone gene. J Cell Physiol 139: 320-328.
58. Pilbeam CC, Klein-Nulend J, Raisz LG (1989): Inhibition by 17β-estradiol of PTH-stimulated resorption and prostaglandin production in cultured neonatal mouse calvariae. Biochem Biophys Res Commun 163:1319-1324.
59. Pivirotto LA, Cissel DS, Keeting PE (1995): Sex hormones mediate interleukin-1β production by human osteoblastic HOBIT cells. Mol Cell Endocrinol 111:67-74.
60. Raeymaekers L. (1993): Quantitative PCR: theoretical considerations with practical implications. Anal Biochem 214:582-585.
61. Rao LG, Wylie JN, Sutherland MSK, Murray TM (1994): 17β-Estradiol and parathyroid hormone potentiate each other's stimulatory effects on alkaline phosphatase activity in SaOS-2 cells in a differentiation-dependent manner. Endocrinology 134:614-620.
62. Rickard D, Russell G, Gowen M (1992): Oestradiol inhibits the release of tumor necrosis factor but not interleukin-6 from adult human osteoblasts in vitro. Osteoporosis Int 2:94-102.
63. Rifas L, Kenney JS, Marcelli M, Pacifici R, Cheng S-L, Dawson LL, Avioli LV (1995): Production of interleukin-6 in human osteoblasts and human bone marrow stromal cells: Evidence that induction by interleukin-1β and tumor necrosis factor-α is not regulated by ovarian steroids. Endocrinology 136:4056-4067.
64. Riggs BL, Melton LJ III (1992): The prevention and treatment of osteoporosis. N Engl J Med 327:620-627.
65. Scatchard G (1949): The attraction of proteins for small molecules and atoms. Ann NY Acad Sci 51:660-672.
66. Shalhoub V, Conlon C, Tassinari M, Quinn C, Partridge N, Stein GS, Lian JB (1992): Glucocorticoids promote development of the osteoblast phenotype by selectively modulating expression of cell growth and differentiation associated genes. J Cell Biochem 50:425-440.
67. Somjen D, Weisman Y, Harell A, Berger E, Kaye AM (1989): Direct and sex-specific stimulation by sex steroids of creatine kinase activity and DNA synthesis in rat bone. Proc Natl Acad Sci USA 86:3361-3365.
68. Stein B, Yang MX (1995): Repression of the interleukin-6 promoter by estrogen receptor is mediated by NF-κB and C/EBPβ. Mol Cell Biol 15:4971-4979.
69. Stein GS, Lian JB (1993): Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblastic phenotype. Endocr Rev 14:424-442.
70. 3 receptors in cultured mouse osteosarcoma cells. Bone 14:7-12.
71. Turner RT, Riggs BL, Spelsberg TC (1994): Skeletal effects of estrogen. Endocr Rev 15:275-300.
72. Verhaar HJJ, Damen CA, Duursma SA, Schieven BAA (1994): A comparison of the action of progestins and estrogens on the growth and differentiation of normal adult human osteoblast-like cells in vitro Bone 15:307-311.
73. Wakeling AE, Bowler J (1992): ICI 182,780, a new antioestrogen with clinical potential. J Steroid Biochem Mol Biol 45:173-177.
74. Watts CKW, King RJB (1994): Overexpression of estrogen receptor in HTB 96 human osteosarcoma cells results in estrogen-induced growth inhibition and receptor cross talk. J Bone Miner Res 9:1251-1258.
75. Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C, Raducha M, Harris H (1986): Isolation and characterization of cDNA encoding a human liver/bone/kidney-type alkaline phosphatase. Proc Natl Acad Sci USA 83:7182-7186.