Quantitative molecular analysis of laser-microdissected paraffin-embedded human tissues

Pathobiology

Volumn 68, Issue 4-5, 2000, Pages 202-208

  Lehmann, Ulrich ^a   Bock, Oliver ^a   Glöckner, Sabine ^a   Kreipe, Hans ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Biopsies, archival; Gene amplification; Laser microdissection; Methylation; PCR, real time; Reverse transcription PCR

Indexed keywords

NUCLEIC ACID; PARAFFIN;

CELL ISOLATION; CHROMOSOME MICRODISSECTION; CONFERENCE PAPER; CONTROLLED STUDY; GENE AMPLIFICATION; GENOME; HETEROZYGOSITY LOSS; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MALIGNANT TRANSFORMATION; POINT MUTATION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TECHNOLOGY;

CELL SEPARATION; DISSECTION; HUMANS; IMMUNOHISTOCHEMISTRY; LASERS; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION;

EID: 0034461996     PISSN: 10152008     EISSN: None     Source Type: Journal    
DOI: 10.1159/000055924     Document Type: Conference Paper

References (30)

1. Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA: Laser capture microdissection. Science 1996;274:998-1001.
2. Schutze K, Lahr G: Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 1998;16:737-742.
3. Wittwer CT, Ririe KM, Andrew RV, David DA, Gundry RA, Balis UJ: The LightCycler: A microvolume multisample fluorimeter with rapid temperature control. Biotechniques 1997;22:176-181.
4. Lie YS, Petropoulos CJ: Advances in quantitative PCR technology: 5′ nuclease assays. Curr Opin Biotechnol 1998;9:43-48.
5. Anzick SL, Kononen J, Walker RL, Azorsa DO, Tanner MM, Guan XY, Sauter G, Kallioniemi OP, Trent JM, Meltzer PS: AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science 1997;277:965-968.
6. An HX, Beckmann MW, Reifenberger G, Bender HG, Niederacher D: Gene amplification and overexpression of CDK4 in sporadic breast carcinomas is associated with high tumor cell proliferation. Am J Pathol 1999;154:113-118.
7. Zhu Y, Qi C, Jain S, Le Beau MM, Espinosa R 3rd, Atkins GB, Lazar MA, Yeldandi AV, Rao MS, Reddy JK: Amplification and overexpression of peroxisome proliferator-activated receptor binding protein (PBP/PPARBP) gene in breast cancer. Proc Natl Acad Sci USA 1999; 96:10848-10853.
8. Lehmann U, Glöckner S, Kleeberger W, von Wasielewski HF, Kreipe H: Detection of gene amplification in archival breast cancer specimens by laser-assisted microdissection and quantitative real-time polymerase chain reaction. Am J Pathol 2000;156:1855-1864.
9. Glöckner S, Lehmann U, Wilke N, Kleeberger W, Länger F, Kreipe H: Amplification of growth regulatory genes in intraductal breast cancer is associated with higher nuclear grade but not with the progression to invasiveness. Lab Invest, in press.
10. Fink L, Seeger W, Ermert L, Hanze J, Stahl U, Grimminger F, Kummer W, Bohle RM: Real-time quantitative RT-PCR after laser-assisted cell picking. Nat Med 1998;4:1329-1333.
11. Luo L, Salunga RC, Guo H, Bittner A, Joy KC, Galindo JE, Xiao H, Rogers KE, Wan JS, Jackson MR, Erlander MG: Gene expression profiles of laser-captured adjacent neuronal subtypes. Nat Med 1999;5:117-122.
12. Jackson DP, Lewis FA, Taylor GR, Boylston AW, Quirke P: Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J Clin Pathol 1990;43:499-504.
13. Stanta G, Schneider C: RNA extracted from paraffin-embedded human tissues is amenable to analysis by PCR amplification. Biotechniques 1991;11:304, 306, 308.
14. Finke J, Fritzen R, Ternes P, Lange W, Dolken G: An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed, paraffin-embedded tissues by PCR. Biotechniques 1993;14:448-453.
15. Ruster B, Zeuzem S, Roth WK: Hepatitis C virus sequences encoding truncated core proteins detected in a hepatocellular carcinoma. Biochem Biophys Res Commun 1996;219: 911-915.
16. Mizuno T, Nagamura H, Iwamoto KS, Ito T, Fukuhara T, Tokunaga M, Tokuoka S, Mabuchi K, Seyma T: RNA from decades-old archival tissue blocks for retrospective studies. Diagn Mol Pathol 1998;7:202-208.
17. Sheils OM, Sweeney EC: TSH receptor status of thyroid neoplasms - TaqMan RT-PCR analysis of archival material. J Pathol 1999;188: 87-92.
18. Godfrey TI, Kim S-H, Chavira M, Ruff DW, Warren RS, Gray JW, Jensen RH: Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction. J Mol Diagn 2000;2:84-91.
19. Jones PA, Laird PW: Cancer epigenetics comes of age. Nat Genet 1999;21:163-167.
20. Momparler RL, Bovenzi V: DNA methylation and cancer. J Cell Physiol 2000;183:145-154.
21. Salem C, Liang G, Tsai YC, Coulter J, Knowles MA, Feng AC, Groshen S, Nichols PW, Jones PA: Progressive increases in de novo methylation of CpG islands in bladder cancer. Cancer Res 2000;60:2473-2476.
22. Ueki T, Toyota M, Sohn T, Yeo CJ, Issa JP, Hurban RH, Goggins M: Hypermethylation of multiple genes in pancreatic adenocarcinoma. Cancer Res 2000;60:1835-1839.
23. Gonzalgo ML, Bender CM, You EH, Glendening JM, Flores JF, Walker GJ, Hayward NK, Jones PA, Fountain JW: Low frequency of p16/CDKN2A methylation in sporadic melanoma: Comparative approaches for methylation analysis of primary tumors. Cancer Res 1997;57:5336-5347.
24. Gonzalgo ML, Jones PA: Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE). Nucleic Acids Res 1997;25:2529-2531.
25. Nguyen TT, Nguyen CT, Gonzales FA, Nichols PW, Yu MC, Jones PA: Analysis of cyclin-dependent kinase inhibitor expression and methylation patterns in human prostate cancers. Prostate 2000;43:233-242.
26. Nguyen TT, Mohrbacher AF, Tsai YC, Groffen J, Heisterkamp N, Nichols PW, Yu MC, Lubbert M, Jones PA: Quantitative measure of c-abl and p15 methylation in chronic myelogenous leukemia: Biological implications. Blood 2000;95:2990-2992.
27. Lo YM, Wong IH, Zhang J, Tein MS, Ng MH, Hjelm NM: Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction. Cancer Res 1999;59:3899-3903.
28. Eads CA, Danenberg KD, Kawakami K, Saltz LB, Blake C, Shibata D, Danenberg PV, Laird PW: MethyLight: A high-throughput assay to measure DNA methylation. Nucleic Acids Res 2000;28:E32.
29. Lehmann U, Hasemeier B, Lilischkis R, Kreipe H: Quantitative analysis of promotor hypermethylation in laser-microdissected archival specimens. Lab Invest, in press.
30. Lockhart DJ, Winzeler EA: Genomics, gene expression and DNA arrays. Nature 2000;405: 827-836.