Genotyping of cagA and vacA, Lewis antigen status, and analysis of the poly-(C) tract in the α(1,3)-fucosyltransferase gene of Irish Helicobacter pylori isolates

FEMS Immunology and Medical Microbiology

Volumn 28, Issue 2, 2000, Pages 113-119

  Ryan, Kieran A ^a   Moran, Anthony P ^a   Hynes, Sean O ^a   Smith, Terry ^a   Hyde, Denise ^b   O'Morain, Colm A ^b   Maher, Majella ^a  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b ADELAIDE AND MEATH HOSPITAL   (Ireland)

Author keywords

cagA; Genotyping; Helicobacter pylori; Lewis antigen; Lipopolysaccharide; Poly (C) tract; vacA

Indexed keywords

CELL MEMBRANE PROTEIN; FUCOSYLTRANSFERASE; LIPOPOLYSACCHARIDE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; BACTERIOPHAGE TYPING; BLOOD GROUP LEWIS SYSTEM; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC ANALYSIS; GENOTYPE; HELICOBACTER PYLORI; IMMUNE RESPONSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; WESTERN BLOTTING;

EID: 0034213614     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(00)00141-3     Document Type: Article

References (43)

1. Dunn B.E., Cohen H., Blaser M.J. Helicobacter pylori. Clin. Microbiol. Rev. 10:1997;720-741.
2. Fallone C.A. Determinants of ethnic or geographical differences in infectivity and transmissibility of Helicobacter pylori. Can. J. Gastroenterol. 13:1999;251-255.
3. Vos M., Warren R.M., van Rensberg C.J., Hauman J.H., Epplen J.T., van Helden P.D. Rapid genomic diversification of Helicobacter pylori. Electrophoresis. 20:1999;1475-1485.
4. Fujimoto S., Marshall B., Blaser M.J. PCR-based restriction fragment length polymorphism typing of Helicobacter pylori. J. Clin. Microbiol. 32:1994;331-334.
5. Marshall D.G., Chua A., Keeling P.W.N., Sullivan D.J., Coleman D.C., Smyth C.J. Molecular analysis of Helicobacter pylori populations in antral biopsies from individual patients using randomly amplified polymorphic DNA (RAPD) fingerprinting. FEMS Immunol. Med. Microbiol. 10:1995;317-324.
6. Atherton J.C., Cao P., Peek R.M., Tummuru M.K.R., Blaser M.J., Cover T.L. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J. Biol. Chem. 270:1995;17771-17777.
7. Censini S., Lange C., Xiang Z., Crabtree J.E., Ghiara P., Borodovsky M., Rappuoli R., Covacci A. cag a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. USA. 93:1996;14648-14653.
8. Xiang Z., Censini S., Bayeli P.F., Telford J.L., Figura P.F., Rappuoli R., Covacci A. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the cytotoxin gene. Infect. Immun. 63:1995;94-98.
9. Pan Z.J., van der Hulst R.W.M., Feller M., Xiao S.D., Tytgat G.N.J., Dankert J., van der Ende A. Equally high prevalences of infection with cagA-positive Helicobacter pylori in Chinese patients with peptic ulcer disease and those with chronic gastritis-associated dyspepsia. J. Clin. Microbiol. 35:1997;1344-1347.
10. Cover T.L., Blaser M.J. Purification and characterisation of the vacuolating toxin from Helicobacter pylori. J. Biol. Chem. 267:1992;10570-10575.
11. Strobel S., Bereswill S., Basig P., Allgaier P., Sonntag H.G., Kist M. Identification and analysis of a new vacA genotype variant of Helicobacter pylori in different patient groups in Germany. J. Clin. Microbiol. 36:1998;1285-1289.
12. van Doorn L.J., Figueiredo L., Sanna R., Pena S., Midolo P., Ng E.K.W., Atherton J.C., Blaser M.J., Quint W.G. Expanding allelic diversity of Helicobacter pylori. J. Clin. Microbiol. 36:1998;2597-2603.
13. Atherton J.C., Peek R.M., Tham K.T., Cover T.L., Blaser M.J. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology. 112:1997;92-99.
14. Moran A.P. Cell surface characteristics of Helicobacter pylori. FEMS Immunol. Med. Microbiol. 10:1995;271-280.
15. Sherbourne R., Taylor D.E. Helicobacter pylori expresses a compex surface carbohydrate, Lewis X. Infect. Immun. 63:1995;4564-4568.
16. Moran A.P. Pathogenic properties of Helicobacter pylori. Scand. J. Gastroenterol. 31:(Suppl. 215):1996;22-31.
17. Wirth H.P., Yang M.Q., Karita M., Blaser M.J. Expression of the human cell-surface glycoconjugates Lewis X and Lewis Y by Helicobacter pylori isolates is related to cagA status. Infect. Immun. 24:1996;4598-4605.
18. Marshall D.G., Hynes S.O., Coleman D.C., O'Morain C.A., Smyth C.J., Moran A.P. Lack of a relationship between Lewis antigen expression and cagA, CagA, vacA, VacA status of Irish Helicobacter pylori isolates. FEMS Immunol. Med. Microbiol. 24:1999;79-90.
19. Tomb J.F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.P., Gill S., Dougherty B.A., Nelson K., Quackenbush J., Zhou L., Kirkness E.W., Peterson S., Loftus B., Richardson D., Dodson R., Khalak H.G., Glodek A., McKenney K., Fitzegerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.M., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature. 388:1997;539-547.
20. Wang G., Rasko D.A., Sherburne R., Taylor D.E. Molecular genetic basis for the variable expression of Lewis Y antigen in Helicobacter pylori: Analysis of the α(1, 2)-fucosyltransferase gene. Mol. Microbiol. 31:1999;1265-1274.
21. Walsh E.J., Moran A.P. Influence of medium composition on the growth and antigen expression of Helicobacter pylori. J. Appl. Microbiol. 83:1997;67-75.
22. Clayton C.L., Kleanthous H., Coates P.J., Morgan D.D., Tabaqchali S. Sensitive detection of Helicobacter pylori by using polymerase chain reaction. J. Clin. Microbiol. 30:1992;192-200.
23. Miehlke S., Kibler K., Kim J.G., Figura N., Small S.M., Graham D.G., Go M.F. Allelic variation in the cagA gene of Helicobacter pylori obtained from Korea compared to the United States. Am. J. Gastroenterol. 97:1996;1322-1325.
24. van Doorn L.J., Figueiredo C., Sanna R., Blaser M.J., Quint W.G.V. Distinct variants of Helicobacter pylori cagA are associated with vacA subtypes. J. Clin. Microbiol. 37:1999;2306-2311.
25. Hitchcock P.J., Brown T.M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:1983;269-277.
26. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
27. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
28. Rudi J., Kolb C., Maiwald M., Kuck D., Sieg A., Galle R.P., Stremmel W. Diversity of Helicobacter pylori vacA and cagA genes and relationship to VacA and CagA protein expression, cytotoxin production and associated diseases. J. Clin. Microbiol. 36:1995;944-948.
29. Pan Z.J., Berg D.E., van der Hulst R.W.M., Su W.W., Raudonikiene A., Xiao S.D., Dankert J., Tytgat G.N.J., van der Ende A. Prevalence of vacuolating cytotoxin production and distribution of distinct vacA alleles in Helicobacter pylori from China. J. Infect. Dis. 178:1998;220-226.
30. Achtman M., Azuma T., Berg D.E., Ito Y., Morelli G., Pan Z.J., Suerbaum S., Thompson S.A., van der Ende A., van Doorn L.J. Recombination and clonal groupings within Helicobacter pylori from different geographical regions. Mol. Microbiol. 32:1999;459-470.
31. van Doorn L.J., Figueiredo C., Megraud F., Pena S., Midolo P., Queiroz D.M.D.M., Carneiro F., Vanderborght B., Pegado M.D.G.F., Sanna R., De Boer W., Schneeberger P.M., Correa P., Ng E.K.W., Atherton J., Blaser M.J., Quint W.G.V. Geographical distribution of vacA allelic types of Helicobacter pylori. Gastroenterology. 116:1999;823-830.
32. Evans D.G., Queirez D.M.M., Mendes E.N., Evans D.J. Helicobacter pylori cagA status and s and m alleles of vacA in isolates from individuals with a variety of Helicobacter pylori-associated gastric diseases. J. Clin. Microbiol. 36:1998;3435-3437.
33. Ito Y., Azuma T., Ito S., Mijami H., Hirai M., Yamazaki Y., Sato F., Kato T., Kohli Y., Kuriyama M. Analysis and typing of the vacA gene from cagA-positive strains of Helicobacter pylori. J. Clin. Microbiol. 35:1997;1710-1714.
34. Takata T., Fujimoto S., Anzai K., Shirotani T., Okada M., Sawae Y., Ono J. Analysis of the expression of CagA and VacA and the vacuolating activity in 167 isolates from patients with either peptic ulcers or non-ulcer dyspepsia. Am. J. Gastroenterol. 93:1998;30-34.
35. Campbell S., Fraser A., Hulliss B., Schmid J., O'Toole P.W. Evidence for ethnic tropism of Helicobacter pylori. Infect. Immun. 65:1997;3708-3712.
36. Evans D.G., Queiriz D.M., Mendes E.N., Svennerholm A.M., Evans D.J. Differences among Helicobacter pylori strains isolated from three different populations and demonstrated by restriction enzyme analysis of an internal fragment of the conserved gene hpaA. Helicobacter. 4:1999;82-88.
37. Covacci A., Telford J.L., Del Giodice G., Parsonnet J., Rappuoli R. Helicobacter pylori virulence and genetic geography. Science. 284:1999;1328-1333.
38. Appelmelk B.J., Simoons-Smit I., Negrini R., Moran A.P., Aspinall G.O., Forte J.G., De Vries T., Quan H., Verboom T., Maaskant J.J., Ghiara P., Kuipers E.J., Bloemena E., Tadema T.M., Townsend R.R., Tyagarajan K., Crothers J.M., Monteiro M.A., Savio A., De Graff J. Potential role of molecular mimicry between Helicobacter pylori lipopolysaccharide and host Lewis blood group antigens in autoimmunity. Infect. Immun. 64:1996;2031-2040.
39. Berg D.E., Hoffman P.S., Appelmelk B.J., Kusters J.G. The Helicobacter pylori genome sequence: Genetic factors for long life in the gastric mucosa. Trends Microbiol. 5:1997;468-474.
40. Faller G., Steininger H., Appelmelk B., Kirchner T. Evidence of novel pathogenic pathways for the formation of antigastric autoantibodies in Helicobacter pylori gastritis. J. Clin. Pathol. 51:1998;244-245.
41. High N.J., Deadman M.E., Moxon E.R. The role of a repetitive DNA motif (5′-CAAT-3′) in the variable expression of the Haemophilus influenzae lipopolysaccharide epitope αGal(1-4)βGal. Mol. Microbiol. 9:1993;1275-1282.
42. Appelmelk B.J., Martin S.L., Monteiro M.A., Clayton C.A., McColm A.A., Zheng P., Verboom T., Maaskant J.J., van den Eijnden D.H., Hokke C.H., Perry M.B., Vandenbroucke-Grauls C.M., Kusters J.G. Phase variation in Helicobacter pylori lipopolysaccharide due to changes in the lengths of poly(C) tracts in α3-fucosyltransferase genes. Infect. Immun. 67:1999;5361-5366.
43. Ryan K.A., Moran A.P., Hynes S.O., Smith T., Hyde D., O'Morain C.A., Maher M. Virulence factors in an Irish Helicobacter pylori population - including a subset of isolates from intestinal metaplasia patients. Gut. 45:(Suppl. 111):1999;31.