RAD51 is required for propagation of the germinal nucleus in Tetrahymena thermophila

Genetics

Volumn 154, Issue 4, 2000, Pages 1587-1596

  Marsh, Thomas C ^a   Cole, Eric S ^b   Stuart, Kathleen R ^b   Campbell, Colin ^a   Romero, Daniel P ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b ST OLAF COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL GROWTH; CONJUGATION; DNA DAMAGE; GENE DISRUPTION; GENE EXPRESSION; MEIOSIS; MITOSIS; PRIORITY JOURNAL; TETRAHYMENA THERMOPHILA;

ANIMALS; BASE SEQUENCE; CELL CYCLE; CONJUGATION, GENETIC; DNA PRIMERS; DNA REPLICATION; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION; RAD51 RECOMBINASE; TETRAHYMENA THERMOPHILA;

CILIOPHORA; ESCHERICHIA COLI; EUKARYOTA; PROTOZOA; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

EID: 0034120506     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (49)

1. ADL, S. M., and J. D. BERGER, 1996 Commitment to division in ciliate cell cycles. J. Eukaryot. Microbiol. 43: 77-86.
2. AKABOSHI, E., Y. INOUE and H. RYO, 1994 Cloning of the cDNA and genomic DNA that correspond to the recA-like gene of Drosophila melanogaster. Jpn. J. Genet. 69: 663-670.
3. ALLEN, S. L., 1967 Cytogenetics of genomic exclusion in Tetrahymena. Genetics 55: 797-822.
4. ALLIS, C. D., M. COLAVITO-SHEPANSKI and M. A. GOROVSKY, 1987 Scheduled and unscheduled DNA synthesis during development in conjugating Tetrahymena. Dev. Biol (Orlando) 124: 469-480.
5. BASILE, G., M. AKER and R. K. MORTIMER, 1992 Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51. Mol. Cell. Biol. 12: 3235-3246.
6. BAUMANN, P., F. E. BENSON and S. C. WEST, 1996 Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell 87: 757-766.
7. BODENBENDER, J., A. PROHASKA, F. JAUKER, H. HIPKE and G. CLEFFMANN, 1992 DNA elimination and its relation to quantities in the macronucleus of Tetrahymena. Dev. Genet. 13: 103-110.
8. BUTLER, D. K., L. E. YASUDA and M.-C. YAO, 1995 An intramolecular recombination mechanism for the formation of the rRNA gene palindrome of Tetrahymena thermophila. Mol. Cell. Biol. 15: 7117-7126.
9. CAMPBELL, C., and D. P. ROMERO, 1998 Identification and characterization of the RAD51 gene from the ciliate Tetrahymena thermophila. Nucleic Acids Res. 26: 3165-3172.
10. CASSIDY-HANLEY, D., J. BOWEN, J. H. LEE, E. COLE, L. A. VERPLANK et al., 1997 Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics 146: 135-147.
11. CHEN, J., D. P. SILVER, D. WALPITA, S. B. CANTOR, A. F. GAZDAR et al., 1998 Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells. Mol. Cell 2: 317-328.
12. COLE, F. S., and T. A. SOELTER, 1997 A mutational analysis of conjugation in Tetrahymena thermophila. 2. Phenotypes affecting middle and late development: third prezygotic nuclear division, pronuclear exchange, pronuclear fusion and postzygotic development. Dev. Biol. (Orlando) 189: 233-245.
13. COLE, E. S., D. CASSIDY-HANLEY, J. HEMISH, J. TUAN and P. J. BRUNS, 1997 A mutational analysis of conjugation in Tetrahymena thermophila. 1. Phenotypes affecting early development - meiosis to nuclear selection. Dev. Biol. (Orlando) 189: 215-232.
14. COYNE, R. S., D. L. CHALKER and M.-C. YAO, 1996 Genome downsizing during ciliate development - nuclear division of labor through chromosome restructuring. Annu. Rev. Genet. 30: 557-578.
15. DEAK, J. C., and F. P. DOERDER, 1998 High frequency intragenic recombination during macronuclear development in Tetrahymena thermophila restores the wild-type SerH1 gene. Genetics 148: 1109-1115.
16. DOERDER, F. P., and L. E. DEBAULT, 1975 Cytofluorimetric analysis of nuclear DNA during meiosis, fertilization and macronuclear development in the ciliate Tetrahymena thermophila, syngen 1. J. Cell Sci. 17: 471-493.
17. DOERDER, F. P., and S. K. SHABATURA, 1980 Genomic exclusion in Tetrahymena thermophila: a cytogenetic and cytofluorometric study. Dev. Genet. 1: 205-218.
18. DRESSER, M. E., D. J. EWING, M. N. CONRAD, A. M. DOMINGUEZ, R. BARSTEAD et al., 1997 DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics 147: 533-544.
19. GAERTIG, J., and M. A. GOROVSKY, 1992 Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants. Proc. Natl. Acad. Sci. USA 89: 9196-9200.
20. GAERTIG, J., L. GU, B. HAI and M. A. GOROVSKY, 1994a High frequency vector-mediated transformation and gene replacement in Tetrahymena. Nucleic Acids Res. 22: 5391-5398.
21. GAERTIG, J., T. H. THATCHER, L. GU and M. A. GOROVSKY, 1994b Electroporation-mediated replacement of a positively and negatively selectable β-tubulin gene in Tetrahymena thermophila. Proc. Natl. Acad. Sci. USA 91: 4549-4553.
22. GAME, J. C., 1983 Radiation-sensitive mutants and repair in yeast, pp. 109-137 in Yeast Genetics: Fundamental and Applied Aspects, edited by J. F. T. SPENCER, D. H. SPENCER and A. R. W. SMITH. Springer-Verlag, New York.
23. GAVIN, R. H., 1965 The effects of heat and cold on cellular development in synchronized Tetrahymena pyriformis WH-6. J. Protozool. 12: 307-318.
24. HAI, B., and M. A. GOROVSKY, 1997 Germ-line knockout heterokaryons of an essential α-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germline nuclei in Tetrahymena thermophila. Proc. Natl. Acad. Sci. USA 94: 1310-1315.
25. KIRK, K. E., B. P. HARMON, I. K. REICHARDT, J. W. SEDAT and E. H. BLACKBURN, 1997 Block in anaphase chromosome separation caused by a telomerase template mutation. Science 275: 1478-1481.
26. LEE, S., J. C., WISNIEWSKI, W. L. DENTLER and D. J. ASAI, 1999 Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila. Mol. Biol. Cell 10: 771-784.
27. MCCORMICK-GRAHAM, M., and D. P. ROMERO, 1996 A single telomerase RNA is sufficient for the synthesis of variable telomeric DNA repeats in ciliates of the genus Paramecium. Mol. Cell. Biol. 16: 1871-1879.
28. MURIS, D. F., K. VREEKEN, A. M. CARR, B. C. BROUGHTON, A. R. LEHMANN et al., 1993 Cloning the RAD51 homologue of Schizosaccharomyces pombe. Nucleic Acids Res. 21: 4586-4591.
29. OGAWA, T., X. YU, A. SHINOHARA and E. H. EGELMAN, 1993 Similarity of the yeast Rad51 filament to the bacterial RecA filament. Science 259: 1896-1899.
30. ORIAS, E., and M. FLACKS, 1975 Macronuclear genetics of Tetrahymena. I. Random distribution of macronuclear gene copies in T. pyriformis, syngen 1. Genetics 79: 187-206.
31. PRESCOTT, D. M., 1994 The DNA of ciliated protozoa. Microbiol. Rev. 58: 233-267.
32. RINALDO, C., S. EDERLE, V. ROCCO and A. LA VOLPE, 1998 The Caenorhabditis elegans RAD51 homolog is transcribed into two alternative mRNAs potentially encoding proteins of different sizes. Mol. Gen. Genet. 260: 289-294.
33. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning: A Laboratory Manual, Ed. 2, Vols. 1-3, edited by C. NOLAN, Gold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
34. SCULLY, R., J. CHEN, A. PLUG, Y. XIAO, D. WEAVER et al., 1997 Association of BRCA1 with Rad51 in mitotic and meiotic cells. Cell 88: 265-275.
35. SHINOHARA, A., H. OGAWA and T. OGAWA, 1992 Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell 69: 457-470.
36. SHINOHARA, A., H. OGAWA, Y. MATSUDA, N. USHIO, K. IKEO et al., 1993 Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat. Genet. 4: 239-243.
37. SONODA, E., M. S. SASAKI, J. M. BUERSTEDDE, O. BEZZUBOVA, A. SHINOHARA et al., 1998 Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death. EMBO J. 17: 598-608.
38. STURZBECHER, H. W., B. DONZELMANN, W. HENNING, U. KNIPPSCHILD and S. BUCHHOP, 1996 p53 is linked directly to homologous recombination processes via Rad51/RecA protein interaction. EMBO J. 15: 1992-2002.
39. SUNG, P., 1994 Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast Rad51 protein. Science 265: 1241-1243.
40. TAKI, T., T. OHNISHI, A. YAMAMOTO, S. HIRAGA, N. ARITA et al., 1996 Antisense inhibition of the RAD51 enhances radiosensitivity. Biochem. Biophys. Res. Commun. 223: 434-438.
41. THOMPSON, L. H., and D. SCHILD, 1999 The contribution of homologous recombination in preserving genome integrity in mammalian cells. Biochimie 81: 87-105.
42. TSUZUKI, T., Y. FUJII, K. SAKUMI, Y. TOMINAGA, K. NAKAO et al., 1996 Targeted disruption of the Rad51 gene leads to lethality in embryonic mice. Proc. Natl. Acad. Sci. USA 93: 6236-6240.
43. VISPE, S., C. CAZAUX, C. LESCA and M. DEFAIS, 1998 Overexpression of Rad51 protein stimulates homologous recombination and increases resistance of mammalian cells to ionizing radiation. Nucleic Acids Res. 26: 2859-2864.
44. WEI, Y., L. L. YU, J. BOWEN, M. A. GOROVSKY and C. D. ALLIS, 1999 Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97: 99-109.
45. WU, M., C. D. ALLIS and M. A. GOROVSKY, 1998 Cell-cycle regulation as a mechanism for targeting proteins to specific DNA sequences in Tetrahymena thermophila. Proc. Natl. Acad. Sci. USA 85: 2205-2209.
46. XIA, S. J., M. A. SHAMMAS and R. J. SHMOOKLER REIS, 1997 Elevated recombination in immortal human cells is mediated by HsRAD51 recombinase. Mol. Cell. Biol. 17: 7151-7158.
47. XU, L., B. M. WEINER and N. KLECKNER, 1997 Meiotic cells monitor the status of the interhomolog recombination complex. Genes Dev. 11: 106-118.
48. YU, G.-L., and E. H. BLACKBURN, 1990 Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila. Mol. Cell. Biol. 10: 2070-2080.
49. YUAN, Z. M., Y. HUANG, T. ISHIKO, S. NAKADA, T. UTSUGISAWA et al., 1998 Regulation of Rad51 function by c-Abl in response to DNA damage. J. Biol. Chem. 273: 3799-3802.