Germ-line knockout heterokaryons of an essential α-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena thermophila

Proceedings of the National Academy of Sciences of the United States of America

Volumn 94, Issue 4, 1997, Pages 1310-1315

  Hai, Bing ^a   Gorovsky, Martin A ^a  

^a UNIVERSITY OF ROCHESTER   (United States)

Author keywords

biolistic transformation; conjugation; paromomycin

Indexed keywords

ALPHA TUBULIN;

ARTICLE; CONTROLLED STUDY; GENE TRANSFER; GENETIC TRANSFORMATION; GERM LINE; HETEROKARYON; MACRONUCLEUS; MATING; MICRONUCLEUS; NONHUMAN; PRIORITY JOURNAL; PROGENY; TETRAHYMENA THERMOPHILA;

ANIMALS; CELL FUSION; CELL NUCLEUS; CROSSES, GENETIC; GENE DOSAGE; GENE TRANSFER TECHNIQUES; GENES, LETHAL; GENES, PROTOZOAN; GENETIC VECTORS; GENOME, PROTOZOAN; HETEROZYGOTE; HOMOZYGOTE; MICRONUCLEUS, GERMLINE; MUTATION; PLOIDIES; TETRAHYMENA THERMOPHILA; TRANSFORMATION, GENETIC; TUBULIN;

TETRAHYMENA; TETRAHYMENA THERMOPHILA;

EID: 0031029916     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.94.4.1310     Document Type: Article

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