Ceramide induces cell death in the human prostatic carcinoma cell lines PC3 and DU145 but does not seem to be involved in Fas-mediated apoptosis

Laboratory Investigation

Volumn 80, Issue 5, 2000, Pages 671-676

  Gewies, Andreas ^a   Rokhlin, Oskar W ^a   Cohen, Michael B ^a,b  

^a UNIVERSITY OF IOWA   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE INHIBITOR; CERAMIDE; CYCLOHEXIMIDE; DNA FRAGMENT; FAS ANTIGEN; SPHINGOMYELIN;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL DEATH; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INHIBITION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROSTATE CARCINOMA; PROTEIN SYNTHESIS;

EID: 0034079149     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: 10.1038/labinvest.3780070     Document Type: Article

References (42)

1. Ariga T, Jarvis WD, and Yu RK (1998). Role of sphingolipid-mediated cell death in neurodegenerative diseases. J Lipid Res 39:1-16.
2. Ashkenazi A and Dixit VM (1998). Death receptors: Signaling and modulation. Science 281:1305-1308.
3. Brenner B, Ferlinz K, Grassme H, Weller M, Koppenhoefer U, Dichgans J, Sandhoff K, Lang F, and Gulbins E (1998). Fas/CD95/Apo-1 activates the acidic sphingomyelinase via caspases. Cell Death Differ 5:29-37.
4. Brugg B, Michel PP, Agid Y, and Ruberg M (1996). Ceramide induces apoptosis in cultured mesencephalic neurons. J Neurochem 66:733-739.
5. Cifone MG, De Maria R, Roncaioli P, Rippo MR, Azuma M, Lanier LL, Santoni A, and Testi R (1994). Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase. J Exp Med 180:1547-1552.
6. Cifone MG, Roncaioli P, De Maria R, Camarda G, Santoni A, Ruberti G, and Testi R (1995). Multiple pathways originate at the Fas/APO-1 (CD95) receptor: Sequential involvement of phosphatidylcholine-specific phospholipase C and acidic sphingomyelinase in the propagation of the apoptotic signal. Embo J 14:5859-5868.
7. Cock JG, Tepper AD, de Vries E, van Blitterswijk WJ, and Borst J (1998). CD95 (Fas/APO-1) induces ceramide formation and apoptosis in the absence of a functional acid sphingomyelinase. J Biol Chem 273:7560-7565.
8. De Maria R, Rippo MR, Schuchman EH, and Testi R (1998). Acidic sphingomyelinase (ASM) is necessary for fas-induced GD3 ganglioside accumulation and efficient apoptosis of lymphoid cells. J Exp Med 187:897-902.
9. Gamen S, Anel A, Pineiro A, and Naval J (1998). Caspases are the main executioners of Fas-mediated apoptosis, irrespective of the ceramide signalling pathway. Cell Death Differ 5:241-249.
10. Gewies A, Pokhlin OW, and Cohen MB (In Press, 2000). Cytochrome C is involved in Fas mediated apoptosis of prostate carcinoma cell lines. Cancer Res
11. Gulbins E, Bissonnette R, Mahboubi A, Martin S, Nishioka W, Brunner T, Baier G, Baier-Bitterlich G, Byrd C, Lang F, Kolesnick R, Altman A, and Green D (1995). FAS-induced apoptosis is mediated via a ceramide-initiated RAS signaling pathway. Immunity 2:341-351.
12. Gulbins E, Szabo I, and Lang F (1996). Physiology of Fas-induced programmed cell death. Cell Physiol Biochem 6:361-375.
13. Hannun YA (1996). Functions of ceramide in coordinating cellular responses to stress. Science 274:1855-1859.
14. Hartfield PJ, Bilney AJ, and Murray AW (1998). Neurotrophic factors prevent ceramide-induced apoptosis downstream of c-Jun N-terminal kinase activation in PC12 cells. J Neurochem 71:161-169.
15. Hirsch T, Marchetti P, Susin SA, Dallaporta B, Zamzami N, Marzo I, Geuskens M, and Kroemer G (1997). The apoptosis-necrosis paradox. Apoptogenic proteases activated after mitochondrial permeability transition determine the mode of cell death. Oncogene 15:1573-1581.
16. Hofmann K and Dixit VM (1998). Ceramide in apoptosis - does it really matter? Trends Biochem Sci 23:374-377.
17. Jarvis WD, Kolesnick RN, Fornari FA, Traylor RS, Gewirtz DA, and Grant S (1994). Induction of apoptotic DNA damage and cell death by activation of the sphingomyelin pathway. Proc Natl Acad Sci USA 91:73-77.
18. Jayadev S, Liu B, Bielawska AE, Lee JY, Nazaire F, Pushkareva M, Obeid LM, and Hannun YA (1995). Role for ceramide in cell cycle arrest. J Biol Chem 270:2047-2052.
19. Li H, Zhu H, Xu CJ, and Yuan J (1998). Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell 94:491-501.
20. McCarthy NJ, Whyte MK, Gilbert CS, and Evan GI (1997). Inhibition of Ced-3/ICE-related proteases does not prevent cell death induced by oncogenes, DNA damage, or the Bcl-2 homologue Bak. J Cell Biol 136:215-227.
21. Medema JP, Scaffidi C, Kischkel FC, Shevchenko A, Mann M, Krammer PH, and Peter ME (1997). FLICE is activated by association with the CD95 death-inducing signaling complex (DISC). Embo J 16:2794-2804.
22. Mengubas K, Fahey AA, Lewin J, Mehta AB, Hoffbrand AV, and Wickremasinghe RG (1999a). Killing of T lymphocytes by synthetic ceramide is by a nonapoptotic mechanism and is abrogated following mitogenic activation. Exp Cell Res 249: 116-122.
23. Mengubas K, Riordan FA, Bravery CA, Lewin J, Owens DL, Mehta AB, Hoffbrand AV, and Wickremasinghe RG (1999b). Ceramide-induced killing of normal and malignant human lymphocytes is by a non-apoptotic mechanism. Oncogene 18:2499-2506.
24. Muzio M, Salvesen GS, and Dixit VM (1997). FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. J Biol Chem 272:2952-2956.
25. Nagata S and Golstein P (1995). The Fas death factor. Science 267:1449-1456.
26. Obeid LM, Linardic CM, Karolak LA, and Hannun YA (1993). Programmed cell death induced by ceramide. Science 259: 1769-1771.
27. Rokhlin OW, Bishop GA, Hostager BS, Waldschmidt TJ, Sidorenko SP, Pavloff N, Kiefer MC, Umansky SR, Glover RA, and Cohen MB (1997a). Fas-mediated apoptosis in human prostatic carcinoma cell lines. Cancer Res 57:1758-1768.
28. Rokhlin OW, Glover RA, and Cohen MB (1998). Fas-mediated apoptosis in human prostatic carcinoma cell lines occurs via activation of caspase-8 and caspase-7. Cancer Res 58: 5870-5875.
29. Rokhlin OW, Hostager BS, Bishop GA, Sidorenko SP, Glover RA, Gudkov AV, and Cohen MB (1997b). Dominant nature of the resistance to Fas-and tumor necrosis factor-alpha-mediated apoptosis in human prostatic carcinoma cell lines. Cancer Res 57:3941-3943.
30. Sawai H, Okazaki T, Yamamoto H, Okano H, Takeda Y, Tashima M, Sawada H, Okuma M, Ishikura H, Umehara H, and Domae N (1995). Requirement of AP-1 for ceramide-induced apoptosis in human leukemia HL-60 cells. J Biol Chem 270:27326-27331.
31. Schue S, Potthoff K, Machleidt T, Berkovic D, Wiegmann K, and Kronke M (1992). TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced "acidic" sphingomyelin breakdown. Cell 71:765-776.
32. Sillence DJ and Allan D (1997). Evidence against an early signalling role for ceramide in Fas-mediated apoptosis. Biochem J 324(Pt 1):29-32.
33. Skowronski EW, Kolesnick RN, and Green DR (1996). Fas-mediated apoptosis and sphingomyelinase signal transduction: The role of ceramide as a second messenger for apoptosis. Cell Death Differ 3:171-176.
34. Tepper AD, Cock JG, de Vries E, Borst J, and van Blitterswijk WJ (1997). CD95/Fas-induced ceramide formation proceeds with slow kinetics and is not blocked by caspase-3/CPP32 inhibition. J Biol Chem 272:24308-24312.
35. Tepper AD, de Vries E, van Blitterswijk WJ, and Borst J (1999). Ordering of ceramide formation, caspase activation, and mitochondrial changes during CD95-and DNA damage-induced apoptosis. J Clin Invest 103:971-978.
36. Tepper CG, Jayadev S, Liu B, Bielawska A, Wolff R, Yonehara S, Hannun YA, and Seldin MF (1995). Role for ceramide as an endogenous mediator of Fas-induced cytotoxicity. Proc Natl Acad Sdi USA 92:8443-8447.
37. Vento R, Giuliano M, Lauricella M, Carabillo M, Di Liberto D, and Tesoriere G (1998). Induction of programmed cell death in human retinoblastoma Y79 cells by C2-ceramide. Mol Cell Biochem 185:7-15.
38. Verheij M, Bose R, Lin XH, Yao B, Jarvis WD, Grant S, Birrer MJ, Szabo E, Zon LI, Kyriakis JM, Haimovitz-Friedman A, Fuks Z, and Kolesnick RN (1996). Requirement for ceramide-initiated SAPK/JNK signalling in stress-induced apoptosis. Nature 380:75-79.
39. Watts JD, Gu M, Patterson SD, Aebersold R, and Polverino AJ (1999). On the complexities of ceramide changes in cells undergoing apoptosis: Lack of evidence for a second messenger function in apoptotic induction. Cell Death Differ 6:105-114.
40. Watts JD, Gu M, Polverino AJ, Patterson SD, and Aebersold R (1997). Fas-induced apoptosis of T cells occurs independently of ceramide generation. Proc Natl Acad Sci USA 94:7292-7296.
41. Westwick JK, Bielawska AE, Dbaibo G, Hannun YA, and Brenner DA (1995). Ceramide activates the stress-activated protein kinases. J Biol Chem 270:22689-22692.
42. Wiegmann K, Schutze S, Machleidt T, Witte D, and Kronke M (1994). Functional dichotomy of neutral and acidic sphingomyelinases in tumor necrosis factor signaling. Cell 78:1005-1015.