Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms

Molecular and Cellular Biology

Volumn 20, Issue 12, 2000, Pages 4199-4209

  Olson, K Amy ^a   Nelson, Chris ^a   Tai, Georgia ^a   Hung, Wesley ^a   Yong, Carl ^a   Astell, Caroline ^a   Sadowski, Ivan ^a,b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b Biochemistry and Molecular Biology   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; INHIBITOR PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PHEROMONE; TRANSCRIPTION FACTOR;

ARTICLE; CARBOXY TERMINAL SEQUENCE; DNA BINDING; GENE ACTIVATION; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; MATING; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION REGULATION;

FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; PHEROMONES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 0034076869     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.12.4199-4209.2000     Document Type: Article

References (43)

1. Ausubel, F. M., R. Brent, K. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1987. Current protocols in molecular biology. Green Publishing Associates/Wiley-Interscience, New York, N.Y.
2. Bardwell, L., J. G. Cook, D. Voora, D. M. Baggott, A. R. Martinez, and J. Thorner. 1998. Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK. Genes Dev. 12:2887-2898.
3. Bardwell, L., J. G. Cook, J. X. Zhu-Shimoni, D. Voora, and J. Thorner. 1998. Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc. Natl. Acad. Sci. USA 95:15400-15405.
4. Carey, M., H. Kakidani, J. Leatherwood, F. Mostashari, and M. Ptashne. 1989. An amino-terminal fragment of GAL4 binds DNA as a dimer. J. Mol. Biol. 209:423-432.
5. Cook, J. G., L. Bardwell, S. J. Kron, and J. Thorner. 1996. Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev. 10:2831-2848.
6. Courchesne, W. E., R. Kunisawa, and J. Thorner. 1989. A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae. Cell 58:1107-1119.
7. Cullin, C., and D. Pompon. 1988. Synthesis of functional mouse cytochromes P-450 and chimeric P-450 P3-1 in the yeast Saccharomyces cerevisiae. Gene 65:203-217.
8. Elion, E. A., J. A. Brill, and G. R. Fink. 1991. FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc. Natl. Acad. Sci. USA 88:9392-9396.
9. Elion, E. A., B. Satterberg, and J. E. Kranz. 1993. FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol. Biol. Cell 4:495-510.
10. Errede, B., and G. Ammerer. 1989. STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. Genes Dev. 3:1349-1361.
11. Fields, S., and I. Herskowitz. 1985. The yeast STE12 product is required for expression of two sets of cell-type specific genes. Cell 42:923-930.
12. Gietz, R. D., and A. Sugino. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-535.
13. Gustin, M. C., J. Albertyn, M. Alexander, and K. Davenport. 1998. MAP kinase pathways in the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62:1264-1300.
14. Hagen, D. C., G. McCaffrey, and G. F. Sprague, Jr. 1991. Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 11:2952-2961.
15. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
16. Hung, W., K. A. Olson, A. Breitklutz, and I. Sadowski. 1997. Characterization of the basal and pheromone-stimulated phosphorylation states of Ste12p. Eur. J. Biochem. 245:241-251.
17. Johnston, S. A., and J. E. Hopper. 1982. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. Proc. Natl. Acad. Sci. USA 79:6971-6975.
18. Leuther, K., and S. A. Johnston. 1992. Nondissociation of GAL4 and GAL80 in vivo after galactose induction. Science 256:1333-1335.
19. Liu, H., C. A. Styles, and G. R. Fink. 1993. Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science 262: 1741-1744.
20. Ma, J., and M. Ptashne. 1987. The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80. Cell 50:137-142.
21. Ma, J., and M. Ptashne. 1987. Deletion analysis of GAL4 defines two transcriptional activating segments. Cell 48:847-853.
22. Madhani, H. D., and G. R. Fink. 1997. Combinatorial control required for the specificity of yeast MAPK signaling. Science 275:1314-1317.
23. Madhani, H. D., C. A. Styles, and G. R. Fink. 1997. MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell 91:673-684.
24. Marmorstein, R., M. Carey, M. Ptashne, and S. C. Harrison. 1992. DNA recognition by GAL4: structure of a protein-DNA complex. Nature 356:408-414.
25. Matsuura, Y., R. D. Possee, H. A. Overton, and D. H. Bishop. 1987. Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. J. Gen. Virol. 68:1233-1250.
26. Peter, M., A. Gartner, J. Horecka, G. Ammerer, and I. Herskowitz. 1993. FAR1 links the signal transduction pathway to the cell cycle machinery in yeast. Cell 73:747-760.
27. Pi, H., C. T. Chien, and S. Fields. 1997. Transcriptional activation upon pheromone stimulation mediated by a small domain of Saccharomyces cerevisiae Ste12p. Mol. Cell. Biol. 17:6410-6418.
28. Platt, A., and R. J. Reece. 1998. The yeast galactose genetic switch is mediated by the formation of a Ga14p-Ga180p-Ga13p complex. EMBO J. 17:4086-4091.
29. Reece, R. J., R. J. Rickles, and M. Ptashne. 1993. Overproduction and single-step purification of GAL4 fusion proteins from Escherichia coli. Gene 126:105-107.
30. Roberts, C. J., B. Nelson, M. J. Marton, R. Stoughton, M. R. Meyer, H. A. Bennett, Y. D. He, H. Dai, W. L. Walker, T. R. Hughes, M. Tyers, C. Boone, and S. H. Friend. 2000. Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles. Science 287:873-880.
31. Sadowski, I., B. Bell, P. Broad, and M. Hollis. 1992. GAL4 fusion vectors for expression in yeast or mammalian cells. Gene 118:137-141.
32. Sadowski, I., J. Ma, S. Triezenberg, and M. Ptashne. 1988. GAL4-VP16 is an unusually potent transcriptional activator. Nature 335:563-564.
33. Sadowski, I., D. Niedbala, K. Wood, and M. Ptashne. 1991. GAL4 is phosphoryiated as a consequence of transcriptional activation. Proc. Natl. Acad. Sci. USA 88:10510-10514.
34. gag-1ps protein-tyrosine kinase distinguished by the expression of v-fps polypeptides in Escherichia coli. Oncogene 1:181-191.
35. Schmitt, M. E., T. A. Brown, and B. L. Trumpower. 1990. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 18:3091-3092.
36. Sengupta, P., and B. H. Cochran. 1991. MAT alpha 1 can mediate gene activation by a-mating factor. Genes Dev. 5:1924-1934.
37. Sengupta, P., and B. H. Cochran. 1990. The PRE and PQ box are functionally distinct yeast pheromone response elements. Mol. Cell. Biol. 10:6809-6812.
38. Song, D., J. W. Dolan, Y. L. Yuan, and S. Fields. 1991. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation. Genes Dev. 5:741-750.
39. Stone, G., and I. Sadowski. 1993. GAL4 is regulated by a glucose-responsive functional domain. EMBO J. 12:1375-1385.
40. Tedford, K., S. Kim, D. Sa, K. Stevens, and M. Tyers. 1997. Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr. Biol. 7:228-238.
41. 1-phase Cdc28 kinase complexes. Mol. Cell. Biol. 13:5659-5669.
42. Yuan, Y. L., and S. Fields. 1991. Properties of the DNA-hinding domain of the Saccharomyces cerevisiae STE12 protein. Mol. Cell. Biol. 11:5910-5918.
43. Yuan, Y. L., I. L. Stroke, and S. Fields. 1993. Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins. Genes Dev. 7:1584-1597.