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A genome-wide inventory of yeast genes that are induced or repressed in response to sporulation. This analysis demonstrates the power of cluster analysis for grouping genes into different pathways. The gene expression patterns during sporulation followed two major stages in meiosis: first, the meiotic prophase; and second, meiotic division and gamete morphogenesis. Novel genes participating in the meiotic pathway were discovered
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The first example of how microarray experiments can contribute to our understanding of adaptation. Three yeast strains were selected by their ability to grow on minimal medium during 250-500 generations. The analysis of the expression patterns in the three evolved strains indicated that increased fitness was acquired by altering regulation of central metabolism such that less glucose is fermented and more glucose is completely oxidized
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Ferea T.L., Botstein D., Brown P.O., Rosenzweig R.F. Systematic changes in gene expression patterns following adaptive evolution in yeast. Proc Natl Acad Sci USA. 96:1999;9721-9726. The first example of how microarray experiments can contribute to our understanding of adaptation. Three yeast strains were selected by their ability to grow on minimal medium during 250-500 generations. The analysis of the expression patterns in the three evolved strains indicated that increased fitness was acquired by altering regulation of central metabolism such that less glucose is fermented and more glucose is completely oxidized.
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The paper unravels the effect of key components of the transcriptional machinery on gene expression via mutant analysis. Distinct sets of genes and a distinct percentage of genes depend on the functions of components of the RNA polymerase II holoenzyme, the general transcription factor TFIID, and the SAGA chromatin modification complex
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Holstege F.C., Jennings E.G., Wyrick J.J., Lee T.I., Hengartner C.J., Green M.R., Golub T.R., Lander E.S., Young R.A. Dissecting the regulatory circuitry of a eukaryotic genome. Cell. 95:1998;717-728. The paper unravels the effect of key components of the transcriptional machinery on gene expression via mutant analysis. Distinct sets of genes and a distinct percentage of genes depend on the functions of components of the RNA polymerase II holoenzyme, the general transcription factor TFIID, and the SAGA chromatin modification complex.
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(1998)
Cell
, vol.95
, pp. 717-728
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Holstege, F.C.1
Jennings, E.G.2
Wyrick, J.J.3
Lee, T.I.4
Hengartner, C.J.5
Green, M.R.6
Golub, T.R.7
Lander, E.S.8
Young, R.A.9
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42
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0032125381
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Transcriptional regulators and the evolution of plant form
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The authors argue that changes in gene regulation underlie the evolution of plant form rather than a change in function of proteins. They strengthen their hypothesis using examples of genes that regulate development in plants and animals. They discuss how ideas about evolution have developed since Darwin's theory
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Doebley J., Lukens L. Transcriptional regulators and the evolution of plant form. Plant Cell. 10:1998;1075-1082. The authors argue that changes in gene regulation underlie the evolution of plant form rather than a change in function of proteins. They strengthen their hypothesis using examples of genes that regulate development in plants and animals. They discuss how ideas about evolution have developed since Darwin's theory.
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(1998)
Plant Cell
, vol.10
, pp. 1075-1082
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Doebley, J.1
Lukens, L.2
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