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Volumn 282, Issue 5389, 1998, Pages 699-705

The transcriptional program of sporulation in budding yeast

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; MESSENGER RNA; TRANSCRIPTION FACTOR;

EID: 0032561246     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5389.699     Document Type: Article
Times cited : (1576)

References (73)
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    • These microscopic observations indicate that meiosis I is initiated by about 5 hours and also provide a measure of the synchrony of the process in this cell population. Two-thirds of the cells in the population went through meiosis I and sporulated (Fig. 2A). The progression of the population through the morphologically defined stages of sporulation was only moderately synchronous, as indicated by the disappearance of mononucleate cells (Fig. 2A), and the synchrony diminished over time. Thus, the apparently sustained changes in expression that we observed may not reflect sustained expression in individual cells but rather the superposition of several stages in the process.
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    • note
    • We are indebted to V. Iyer for help with genome array production. We also thank P. Spellman and S. Biggins for their help; F. Banuett, N. Kleckner, and D. Meling for useful discussions; and B. Byers, S. Klapholz, I. H. Herskowitz, Y. Kassir, and A. Mitchell for comments on the manuscript. Strains of the SK1 background and the hisG-URA3 disruption cassette were kind gifts from N. Kleckner. S.C. and I.H. are supported by National Institutes of Health (NIH) grant AI18738. J.D. and P.O.B. are supported by the Howard Hughes Medical Institute (HHMI) and NIH grant HG00450. M.E. is supported by a postdoctoral fellowship from the Alfred E. Sloan Foundation. P.O.B. is an Associate Investigator of the HHMI. J.M. and D.B. are supported by NIH grant GM46406. This work was supported by grants from NIH and by HHMI.


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