Divalent forms of CC49 single-chain antibody constructs in Pichia pastoris: Expression, purification, and characterization

Journal of Biochemistry

Volumn 127, Issue 5, 2000, Pages 829-836

  Goel, Apollina ^a   Beresford, Guy W ^d   Colcher, David ^e   Pavlinkova, Gabriela ^b   Booth, Barbara J M ^b   Baranowska Kortylewicz, Janina ^c   Batra, Surinder K ^a,b,c  

^a Dept of Biochem and Molec Biology ^*  (United States)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^c UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^d EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e Coulter Pharmaceutical Inc ^*  (United States)

Author keywords

Colon carcinoma xenografts; Pharmacokinetics; Single chain Fv; Valency; Yeast

Indexed keywords

ANTINEOPLASTIC AGENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN;

ANTINEOPLASTIC ACTIVITY; ARTICLE; COLON CARCINOMA; DRUG ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOREACTIVITY; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SURFACE PLASMON RESONANCE; XENOGRAFT;

ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 0034074997     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022676     Document Type: Article

References (39)

1. Bird, R.E., Hardman, K.D., Jacobson, J.W., Johnson, S., Kaufman, B.M., Lee, S.M., Lee, T., Pope, S.H., Riordan, G.S., and Whitlow, M. (1988) Single-chain antigen-binding proteins. Science 242, 423-426
2. Colcher, D., Bird, R., Roselli, M., Hardman, K.D., Johnson, S., Pope, S., Dodd, S.W., Pantoliano, M.W., Milenic, D.E., and Schlom, J. (1990) In vivo tumor targeting of a recombinant single-chain antigen-binding protein. J. Natl. Cancer Inst. 82, 1191-1197
3. Milenic, D.E., Yokota, T., Filpula, D.R., Finkelman, M.A., Dodd, S.W., Wood, J.F., Whitlow, M., Snoy, P., and Schlom, J. (1991) Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res. 51, 6363-6371
4. Adams, G.P., McCartney, J.E., Tai, M.S., Oppermann, H., Huston, J.S., Stafford, W.F.d., Bookman, M.A., Fand, I., Houston, L.L., and Weiner, L.M. (1993) Highly specific in vivo tumor targeting by monovalent and divalent forms of 741F8 anti-c-erbB-2 single-chain Fv. Cancer Res. 53, 4026-4034
5. King, D.J., Turner, A., Farnsworth, A.P., Adair, J.R., Owens, R.J., Pedley, R.B., Baldock, D., Proudfoot, K.A., Lawson, A.D., Beeley, N.R., Millar, K., Millican, T.A., Boyce, B.A., Antoniw, P., Mountain, A., Begent, R.H.J., Shochat, D., and Yarranton, G.T. (1994) Improved tumor targeting with chemically cross-linked recombinant antibody fragments. Cancer Res. 54, 6176-6185
6. Wu, A.M., Chen, W., Raubitschek, A., Williams, L.E., Neumaier, M., Fischer, R., Hu, S.Z., Odom-Maryon, T., Wong, J.Y., and Shively, J.E. (1996) Tumor localization of anti-CEA single-chain Fvs: improved targeting by non-covalent dimers. Immunotechnology 2, 21-36
7. Hu, S., Shively, L., Raubitschek, A., Sherman, M., Williams, L.E., Wong, J.Y., Shively, J.E., and Wu, A.M. (1996) Minibody: A novel engineered anti-carcinoembryonic antigen antibody fragment (single-chain Fv-CH3) which exhibits rapid, high-level targeting of xenografts. Cancer Res. 56, 3055-3061
8. Mayer, A., Chester, K.A., Bhatia, J., Pedley, R.B., Read, D.A., Boxer, G.M., and Begent, R.H. (1998) Exemplifying guidelines for preparation of recombinant DNA products in phase I trials in cancer: preparation of a genetically engineered anti-CEA single chain Fv antibody. Eur. J. Cancer 34, 968-976
9. Beresford, G.W., Pavlinkova, G., Booth, B.J., Batra, S.K., and Colcher, D. (1999) Binding characteristics and tumor targeting of a covalently linked divalent CC49 single-chain antibody. Int. J. Cancer 81, 911-917
10. Pluckthun, A. and Pack, P. (1997) New protein engineering approaches to multivalent and bispecific antibody fragments. Immunotechnology 3, 83-105
11. Eldin, P., Pauza, M.E., Hieda, Y., Lin, G., Murtaugh, M.P., Pentel, P.R., and Pennell, C.A. (1997) High-level secretion of two antibody single chain Fv fragments by Pichia pastoris. J. Immunol. Methods 201, 67-75
12. Casey, J.L., Keep, P.A., Chester, K.A., Robson, L., Hawkins, R.E., and Begent, R.H. (1995) Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography. J. Immunol. Methods 179, 105-116
13. Shusta, E.V., Raines, R.T., Pluckthun, A., and Wittrup, K.D. (1998) Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nat. Biotechnol. 16, 773-777
14. Cregg, J.M., Vedvick, T.S., and Raschke, W.C. (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology (NY) 11, 905-910
15. Cregg, J. (1999) in Gene Expression Systems: Using Nature for the Art of Expression, pp. 157-191, Academia press, San Diego
16. Jefferis, R., Lund, J., and Goodall, M. (1995) Recognition sites on human IgG for Fc gamma receptors: the role of glycosylation. Immunol. Lett. 44, 111-117
17. Begent, R. (1993) Facilitating phase I trials of products of recombinant DNA technology. Eur. J. Cancer 13, 1803-1804
18. Zhu, Z., Rockwell, P., Lu, D., Kotanides, H., Pytowski, B., Hicklin, D.J., Bohlen, P., and Witte, L. (1998) Inhibition of vascular endothelial growth factor-induced receptor activation with anti-kinase insert domain-containing receptor single-chain antibodies from a phage display library. Cancer Res. 58, 3209-3214
19. Pavlinkova, G., Beresford, G.W., Booth, B.J., Batra, S.K., and Colcher, D. (1999) Pharmacokinetics and biodistribution of engineered single-chain antibody constructs of MAb CC49 in colon carcinoma xenografts. J. Nucl. Med. 40, 1536-1546
20. Pavlinkova, G., Beresford, G., Booth, B.J., Batra, S.K., and Colcher, D. (1999) Charge-modified single chain antibody constructs of monoclonal antibody CC49: generation, characterization, pharmacokinetics, and biodistribution analysis. Nucl. Med. Biol. 26, 27-34
21. Pantoliano, M.W., Bird, R.E., Johnson, S., Asel, E.D., Dodd, S.W., Wood, J.F., and Hardman, K.D. (1991) Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli. Biochemistry 30, 10117-10125
22. Lowry, O., Rosenbrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement by Folin phenol. J. Biol. Chem. 193, 265-275
23. Laemmli, U. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
24. Colcher, D., Zalutsky, M., Kaplan, W., Kufe, D., Austin, F., and Schlom, J. (1983) Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody. Cancer Res. 43, 736-742
25. Karlsson, R., Michaelsson, A., and Mattsson, L. (1991) Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system. J. Immunol. Methods 145, 229-240
26. Tom, B., Rutzky, L.P., Jakstys, M.M., Oyasu, R., Kaye, C.I., and Kahan, B.D. (1976) Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12, 180-191
27. Colcher, D., Goel, A., Pavlinkova, G., Beresford, G., Booth, B., and Batra, S.K. (1999) Effects of genetic engineering on the pharmacokinetics of antibodies. Q. J. Nucl. Med. 43, 132-139
28. Behr, T.M., Becker, W.S., Bair, H.J., Klein, M.W., Stuhler, C.M., Cidlinsky, K.P., Wittekind, C.W., Scheele, J.R., and Wolf, F.G. (1995) Comparison of complete versus fragmented technetium-99m-labeled anti-CEA monodonal antibodies for immunoscintigraphy in colorectal cancer. J. Nucl. Med. 36, 430-441
29. Begent, R.H., Verhaar, M.J., Chester, K.A., Casey, J.L., Green, A.J., Napier, M.P., Hope-Stone, L.D., Cushen, N., Keep, P.A., Johnson, C.J., Hawkins, R.E., Hilson, A.J., and Robson, L. (1996) Clinical evidence of efficient tumor targeting based on single-chain Fv antibody selected from a combinatorial library. Nat. Med. 2, 979-984
30. Hannig, G. and Makrides, S.C. (1998) Strategies for optimizing heterologous protein expression in Escherichla coli. Trends Biotechnol. 16, 54-60
31. Skerra, A. (1993) Bacterial expression of immunoglobulin fragments. Curr. Opin. Immunol. 5, 256-262
32. Luo, D., Mah, N., Wishart, D., Zhang, Y., Jacobs, F., and Martin, L. (1996) Construction and expression of bi-functional proteins of single-chain Fv with effector domains. J. Biochem. 120, 229-232
33. FitzGerald, K., Holliger, P., and Winter, G. (1997) Improved tumour targeting by disulphide stabilized diabodies expressed in Pichia pastoris. Protein Eng. 10, 1221-1225
34. Luo, D., Geng, M., Noujaim, A.A., and Madiyalakan, R. (1997) An engineered bivalent single-chain antibody fragment that increases antigen binding activity. J. Biochem. 121, 831-834
35. Magliani, W., Conti, S., de Bernardis, F., Gerloni, M., Bertolotti, D., Mozzoni, P., Cassone, A., and Polonelli, L. (1997) Therapeutic potential of antiidiotypic single chain antibodies with yeast killer toxin activity. Nat. Biotechnol. 15, 155-158
36. McGregor, D.P., Molloy, P.E., Cunningham, C., and Harris, W.J. (1994) Spontaneous assembly of bivalent single chain antibody fragments in Escherichia coli. Mol. Immunol. 31, 219-226
37. Whitlow, M., Bell, B.A., Feng, S.L., Filpula, D., Hardman, K.D., Hubert, S.L., Rollence, M.L., Wood, J.F., Schott, M.E., Milenic, D.E., Yokota, T., and Schlom, J. (1993) An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability. Protein Eng. 6, 989-995
38. Whitlow, M., Filpula, D., Rollence, M.L., Feng, S.L., and Wood, J.F. (1994) Multivalent Fvs: characterization of single-chain Fv oligomers and preparation of a bispecific Fv. Protein Eng. 7, 1017-1026
39. Behr, T.M., Sharkey, R.M., Juweid, M.E., Blumenthal, R.D., Dunn, R.M., Griffiths, G.L., Bair, H.J., Wolf, F.G., Becker, W.S., and Goldenberg, D.M. (1995) Reduction of the renal uptake of radiolabeled monoclonal antibody fragments by cationic amino acids and their derivatives. Cancer Res. 55, 3825-3834