Construction and expression of bi-functional proteins of single-chain Fv with effector domains

Journal of Biochemistry

Volumn 120, Issue 2, 1996, Pages 229-232

  Luo, Dong ^a,b   Mah, Nancy ^a   Wishart, David ^b   Zhang, Yi ^a   Jacobs, Fred ^a   Martin, Luis ^a  

^a Biomira Inc   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Antibody engineering; Bi functional; Disulfide bond; Pichia pastoris; Single chain Fv

Indexed keywords

BIOTIN; CYSTEINE; HYBRID PROTEIN; IDIOTYPIC ANTIBODY; METALLOTHIONEIN; RECOMBINANT PROTEIN;

ANTIBODY STRUCTURE; ANTIGEN BINDING; ARTICLE; CARBOXY TERMINAL SEQUENCE; DISULFIDE BOND; ENDOMYCETALES; GENETIC ENGINEERING; IMMUNOGLOBULIN VARIABLE REGION; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN SECRETION; RADIOIMMUNOASSAY;

PICHIA PASTORIS; SACCHAROMYCETALES;

EID: 0029841258     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021402     Document Type: Article

References (22)

1. Milenic, D.E., Yokota, T., Filpula, D.R., Finkelman, M.A.J., Dodd, S.W., Wood, J.F., Withlow, M., Snoy, P., and Schlom, J. (1991) Construction, binding properties, metabolism and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res. 51, 6363-6371
2. Huston, J.S., Levinson, D., Mudgett-Hunter, M., Tai, M.S., Novotny, J., Margolies, M.N., Ridge, R.J., Bruccoleri, R.E., Haber, E., Crea, R., and Oppermann, H. (1988) Protein engineering of antibody binding sites: Recovery of specific activity of an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. USA 85, 5879-5883
3. Bird, R.E., Hardman, K.D., Jacobson, J.W., Johnson, S., Kaufman, B.M., Lee, S.-M., Lee, T., Pope, S.H., Riordan, G.S., and Withlow, M. (1988) Single-chain antigen-binding proteins. Science 242, 423-426
4. Yokoda, T., Milenic, D.E., Whitlow, M., and Schlom, J. (1992) Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. Cancer Res. 52, 3402-3408
5. Colcher, D.R., Bird, R.E., Roselli, M., Hardman, K.D., Johnson, S., Pope, S., Dodd, S.W., Pantoliano, M.W., Milenic, D.E., and Schlom, J. (1990) In vivo tumor targeting of a recombinant single-chain antigen-binding protein. J. Natl Cancer Inst. 82, 1191-1197
6. Cregg, J.M., Vedvick, T.S., and Raschke, W.C. (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Bio/Technology 11, 905-910
7. Samuel, J., Noujaim, A.A., Willans, D.J., Brzezinska, G., Haines, D.M., and Longenecker, B.M. (1989) A novel marker for basal (stem) cells of mammalian stratified sequamous epithelia and squamous cell carcinomas. Cancer Res. 49, 2465-2470
8. Ding, L., Samuel, J., MacLean, G.D., Noujaim, A.A., Diener, E., and Longenecker, B.M. (1990) Effective drug-antibody targeting using a novel monoclonal antibody against the proliferative compartment of mammalian squamous carcinoma. Cancer Immunol. Immunother. 31, 105-109
9. Jacobs, F.A., Romeyer, F.M., Beauchemin, M., and Brousseau, R. (1989) Human metallothionein-II is synthesized as a stable membrane-localized fusion protein in Escherichia coli. Gene 83, 93-103
10. Kagi, J.H. and Schaffer, A. (1988) Biochemistry of metallothionein. Biochemistry 27, 8509-8515
11. Yoshida, A., Kaplan, B.E., and Kimura, M. (1979) Metal-binding and detoxification effect of synthetic oligopeptides containing three cysteinyl residues. Proc. Natl. Acad. Sci. USA 76, 486-490
12. Knight, L.C., Radcliffe, R., Maurer, A.H., Rodwell, J.D., and Alvarez, V.L. (1994) Thrombus imaging with technetium-99m synthetic peptides based upon the binding domain of a monoclonal antibody to activated platelets. J. Nucl. Med. 35, 282-288
13. Luc, D., Mah, N., Krantz, M., Wilde, K., Wishard, D., Jacobs, F., and Martin, L. (1995) Vl-linker-Vh orientation-dependent expression of single chain Fv containing an engineered disulfidestabilized bond in the frame regions. J. Biochem. 118, 825-831
14. Cwirla, S.E., Peters, E.A., Barrett, R.W., and Dower, W.J. (1990) Peptides on phage: A vast library of peptides for identifying ligands. Proc. Natl. Acad. Sci. USA 87, 6378-6382
15. Saggio, I. and Laufer, R. (1993) Biotin binders selected from a random peptide library expressed on phage. Biochem. J. 293, 613-616
16. Wilchek, M. and Bayer, E. (1990) Methods in Enzymology: Avidin-Biotin Technology Vol. 184, Academic Press, San Diego
17. Pastan, I. and FitzGerald, D. (1991) Recombinant toxins for cancer treatment. Science 254, 1173-1177
18. Chaudhary, V.K., Queen, C., Junghans, R.P., Waldmann, T.A., FitzGerald, D.J., and Pastan, I. (1989) A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin. Nature 339, 394-397
19. Batra, J.K., Kasprzyk, P.G., Bird, R.E., Pastan, I., and King, R.C. (1992) Recombinant anti-erbB2 immunotoxins containing Pseudomonas exotoxin. Proc. Natl. Acad. Sci. USA 89, 5867-5871
20. Brinkmann, U., Pai, L.H., FitzGerald, D.J., Willingham, M., and Pastan, I. (1991) B3(Fv)-PE38KDEL, a single chain immunotoxin that causes complete regression of a human carcinoma in mice. Proc. Natl. Acad. Sci. USA 88, 8616-8620
21. Newton, D.L., Nicholls, P.J., Rybak, S.M., and Youle, R.J. (1994) Expression and characterization of recombinant human eosinophil-derived neurotoxin and eosinophil-derived neurotoxin-anti-transferrin receptor sFv. J. Biol. Chem. 43, 26739-26745
22. Hayden, M.S., Linsley, P.S., Gayle, M.A., Bajorath, J., Brady, W.A., Norris, N.A., Fell, H.P., Ledbetter, J.A., and Gilliland, L.K. (1994) Single-chain mono- and bispecific antibody derivatives with novel biological properties and antitumour activity from COS cell transient expression system. Ther. Immunol. 1, 3-