The mechanism of resistance to sterol 14α demethylation inhibitors in a mutant (Erg 40) of Ustilago maydis

Pest Management Science

Volumn 56, Issue 3, 2000, Pages 257-263

  Butters, Jenny A ^a   Zhou, Ming C ^b   Hollomon, Derek W ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

DMI resistance; Hygromycin resistance; Sterols; Ustilago maydis

Indexed keywords

FUNGICIDE; HYGROMYCIN; STEROL;

ARTICLE; BIOSYNTHESIS; CHEMICAL ANALYSIS; DEMETHYLATION; GENE MUTATION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT; SEQUENCE ANALYSIS; USTILAGO MAYDIS; YEAST;

EID: 0034052420     PISSN: 1526498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1526-4998(200003)56:3<257::AID-PS131>3.0.CO;2-B     Document Type: Article

References (28)

1. Koller W, Antifungal agents with target sites in sterol functions and biosynthesis, in Target sites of fungicide action ed by Koller W, CRC Press, Boca Raton, Florida, USA. pp 119-206 (1992).
2. Hollomon DW, Resistance to azole fungicides in the field. Biochem Soc Trans 21:1047-1051 (1993).
3. DeWaard MA and Van Nistelrooy JGM, Accumulation of SBI fungicides in wild-type and fenarimol-resistant isolates of Penicillium italicum. Pestic Sci 22:371-382 (1998).
4. Wellman H and Schauz K, DMI-resistance in Ustilago maydis II. Effect of triadimefon on regenerating protoplasts and analysis of fungicide uptake. Pestic Biochem Physiol 46:55-64 (1993).
5. Watson PF, Rose ME, Ellis SW, England H and Kelly SL, Defective sterol C5-6 desaturation and azole resistance: a new hypothesis for the mode of action of azole antifungals. Biochem Biophys Res Commun 164:1170-1175 (1989).
6. 450 SG1: complete primary structures deduced from cloned DNAs. Biochem Biophys Res Commun 155:317-323 (1988).
7. Van den Brink HJM, Van Nistelrooy JGM, De Waard MA, Van den Hondel CAMJJ and Van Gorcom RFM, Increased resistance to the 14α demethylase inhibitors (DMIs) in Aspergillus niger by co-expression of the Penicillium italicum eburicol 14α demethylase (cyp51) gene and the Aspergillus niger cytochrome P450 reductase (cpr A) gene. J Biotech 49:13-18 (1998).
8. Hitchcock CA, Resistance of Candida albicans to azole antifungal agents. Biochem Soc Trans 21:1039-1047 (1998).
9. Delye C, Laigret F and Corio-Costet M, A mutation in the 14α-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Appl Environ Microbiol 63:2966-2970 (1997).
10. Sanglard D, Ischer F, Koymans L and Bille J, Amino acid substitutions in the cytochrome P-450 lanosterol 14α-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents. Antimicro Agents Chemo 42:241-253 (1998).
11. Nakaune R, Adachi K, Nawata O, Tomiyama M, Akutsu K and Hibi T, A novel ATP-binding cassette transporter involved in multidrug resistance in the phytopathogenic fungus Penicillium digitatum. Appl Environ Microbiol 64:3983-3988 (1998).
12. Walsh RC and Sisler HD, A mutant of Ustilago maydis deficient in sterol C-14 demethylation: characteristics and sensitivity to inhibitors of ergosterol biosynthesis. Pestic Biochem Physiol 18:122-131 (1982).
13. 14DM activity is hypersensitive to azole fungicides. Fungal Genetics and Biology 20:84-88 (1996).
14. 450 structural gene from Saccharomyces cerevisiae. Gene 45:237-242 (1986).
15. Vogel HJ, Distribution of lysine pathways among fungi: evolutionary implications. Am Nat 98:435 (1964).
16. Kinal H, Tao J and Bruenn JA, An expression vector for the phytopathogenic fungus, Ustilago maydis. Gene 98:129-134 (1991).
17. Butters JA and Hollomon DW, Suitability of an azole mutant of Ustilago maydis for sterol 14α demethylase complementation studies, in Modern Fungicides and Antifungal Compounds. ed by Lyr H, Russell PE and Sisler HD, Intercept, Andover. pp 313-318 (1996).
18. Loeffler RST and Hayes AL, Sterols of the plant pathogenic fungi Botryris cinerea and Pyrenophora teres. Phytochemistry 29:3423-3425 (1990).
19. Wang J, Holden DW and Leong SA, Gene transfer system for the phytopathogenic fungus Ustilago maydis. Proc. Natl Acad Sci USA 85:865-869 (1988).
20. Feinberg AP and Vogelstein B, A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6-13 (1983).
21. Chen EY and Seeburg PH, Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165-170 (1985).
22. 14DM). FEMS Microbiology Letters, 369-373 (1998).
23. Bullock WO, Fernandez JM and Short JM, XL1-Blue-A high efficiency plasmid transforming RccA Escherichia coli strain with beta-galactosidase selection. Biotechniques 5:376-379 (1987).
24. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH and Van den Hondel CAMJJ, Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56:117-124 (1987).
25. Cooley RN, Shaw RK, Franklin FCH and Caten CE, Transformation of the phytopathogenic fungus Septoria nodorum to hygromycin B resistance. Current Genet 13:383-389 (1988).
26. Hargreaves JA and Keon JPR, Isolation of an Ustilago maydis ERG11 gene and its expression in a mutant deficient in sterol 14α demethylase activity. FEMS Letters 139:203-207 (1996).
27. Rodriguez RJ and Parks LW, Structural and physiological features of sterols necessary to satisfy bulk membrane and sparking requirements in yeast sterol auxotrophs. Arch Biochem Biophys 225:861-871 (1983).
28. Dawson PA, Metherall JE, Ridgway ND, Brown MS and Goldstein JL, Genetic distinction between sterol-mediated transcriptional and posttranscriptional control of 3-hydroxy-3-methylglutaryl-coenzyme A reductase. J Biol Chem 266:9128-9134 (1991).