A mutation in the 14α-Demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor

Applied and Environmental Microbiology

Volumn 63, Issue 8, 1997, Pages 2966-2970

  Délye, Christophe ^a   Laigret, Frédéric ^a   Corio Costet, Marie France ^a  

^a CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; DEMETHYLATION; ENZYME ACTIVITY; FUNGAL GENETICS; NONHUMAN; STEROL SYNTHESIS;

ERYSIPHALES; FUNGI; NECATOR; UNCINULA NECATOR; VITIS SP.;

EID: 0042544006     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.8.2966-2970.1997     Document Type: Article

References (37)

1. 14DM) in the rat liver. Biochem. Biophys. Res. Commun. 201: 1320-1326.
2. 14DM) is one of the most ancient and conserved P450 species. J. Biochem. 119:926-933.
3. Buchenauer, H. 1977. Mode of action of triadimefon in Ustilago avenae. Pestic. Biochem. Physiol. 7:309-320.
4. Chen, C., V. F. Kalb, T. G. Turi, and J. C. Loper. 1988. Primary structure of the cytochrome P450 lanosterol 14α-demethylase gene from Candida tropicalis. DNA 9:617-626.
5. Davidse, L. C. 1988. Benzimidazole fungicides: mechanism of action and resistance, p. 25-27. In C. J. Delp (ed.). Fungicide resistance in North America. APS Press, St. Paul, Minn.
6. Debieu, D., M.-F. Corio-Costet, H. Steva, C. Malosse, and P. Leroux. 1995. Sterol composition of the vine powdery mildew fungus Uncinula necator: comparison of triadimenol-sensitive and resistant strains. Phytochemistry 39:293-300.
7. Délye, C., and M.-F. Corio-Costet. 1994. Resistance of grape powdery mildew (Uncinula necator) to triadimenol, a sterol biosynthesis inhibitor: biochemical characterization of sensitive and resistant strains, p. 87-91. In S. Heaney, D. Slawson, D. W. Hollomon, M. Smith, P. E. Russels, and D. W. Parry (ed.), Fungicide resistance. British Crop Protection Council, Surrey, United Kingdom.
8. Délye, C., M.-F. Corio-Costet, and F. Laigret. 1995. A RAPD assay for strain typing of the biotrophic grape powdery mildew fungus Uncinula necator using DNA extracted from the mycelium. Exp. Mycol. 19:234-237.
9. Délye C., F. Laigret, and M.-F. Corio-Costet. Cloning and sequence analysis of the eburicol 14α-demethylase gene of the obligate biotrophic grape powdery mildew fungus. Gene, in press.
10. De Waard, M. A. 1993. Recent developments in fungicides, p. 11-19. In J. C. Zadoks (ed.), Modern crop protection and perspectives. Wageningen Pers, Wageningen, The Netherlands.
11. De Waard, M. A. 1994. Resistance to fungicides which inhibit sterol 14α-demethylation, an historical perspective, p. 3-10. In S. Heaney, D. Slawson, D. W. Hollomon, M. Smith, P. E. Russels, and D. W. Parry (ed.), Fungicide resistance. British Crop Protection Council, Surrey, United Kingdom.
12. De Waard, M. A. 1996. Molecular genetics of resistance in fungi to azole fungicides. ACS Symp. Ser. 645:62-71.
13. De Waard, M. A., E. M. C. Kipp, N. M. Horn, and J. G. M. Van Nistelrooy. 1986. Variations in sensitivity to fungicides which inhibit ergosterol biosynthesis in wheat powdery mildew. Neth. J. Plant Pathol. 92:21-32.
14. Gadher, P., E. I. Mercer, B. C. Baldwin, and T. E. Wiggins. 1983. A comparison of the potency of some fungicides as inhibitors of sterol 14-demethylation. Pestic. Biochem. Physiol. 19:1-10.
15. Geber, A., C. A. Hitchcock, J. E. Swartz, F. S. Pullen, K. E. Marsden, K. J. Kwon-Chun, and J. E. Benett. 1995. Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility. Antimicrob. Agents Chemother. 39:2708-2717.
16. Golembievski, R. C., J. R. Vargas, Jr., A. L. Jones, and A. R. Detweiler. 1995. Detection of demethylation inhibitor (DMI) resistance in Sclerotinia homeocarpa populations. Plant Dis. 79:491-493.
17. Hargreave, J. A., and J. P. R. Keon. 1996. Isolation of an Ustilago maydis ERG11 gene and its expression in a mutant deficient in sterol 14α-demethylase activity. FEMS Microbiol. Lett. 139:203-207.
18. Hitchcock, C. A. 1993. Resistance of Candida albicans to azole antifungal agents. Biochem. Soc. Trans. 21:1039-1047.
19. Hitchcock, C. A., K. J. Barett-Bee, and N. J. Russell. 1987. Inhibition of 14α-sterol demethylase activity in Candida albicans Darlington does not correlate with resistance to azole. J. Med. Vet. Mycol. 25:329-333.
20. Kalb, V. F., C. W. Woods, T. G. Turi, C. R. Dey, T. R. Sutter, and J. C. Loper. 1987. Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae. DNA 6:529-537.
21. Kirsch D R., M. H. Lai, and J. O'Sullivan. 1988. Isolation of the gene for cytochrome P450 L1A1 (lanosterol 14α-demethylase) from Candida albicans. Gene 68:229-237.
22. Kuck, K. H., and H. Scheinpflug. 1986. Biology of sterol-biosynthesis inhibiting fungicides, p. 65-96. In G. Haug and H. Hoffman (ed.), Chemistry of plant protection, vol. 1. Springer-Verlag KG, Berlin, Germany.
23. Lamb, D. C., A. Corran, B. C. Baldwin, J. Kwon-Chung, and S. L. Kelly. 1995. Resistant P45051A1 activity in azole antifungal tolerant Cryptococcus neoformans from AIDS patients. FEBS Lett. 268:326-330.
24. Ouimette, D. G., and W. D. Gubler. 1990. Sensitivity of grape powdery mildew isolates from California towards fenarimol, myclobutanil and triadimefon. Phytopathology 80:975. (Abstract.)
25. 24a. Rhoads, D. D., and D. J. Roufa (Kansas State University). Personal communication.
26. Schepers, H. T. A. M. 1983. Decreased sensitivity of Sphaerotheca fuliginea to fungicides which inhibit ergosterol biosynthesis. Neth. J. Plant Pathol. 89: 185-187.
27. Sloane D. L., O.-Y. So, R. Leung, L. E. Scarafia, N. Saldou, K. Jarnagin, and D. C. Swinney. 1995. Cloning and functional expression ot the cDNA encoding rat lanosterol 14α-demethylase. Gene 161:243-248.
28. Sommer, R., and D. Tautz. 1989. Minimal homology requirements for PCR primers. Nucleic Acids Res. 17:6749.
29. Sommer, S. S., A. R. Groszbar, and C. D. K. Bottema. 1992. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single base-pair changes. BioTechniques 12:82-87.
30. Steva, H. 1994. Evaluating anti-resistance strategies for control of Uncinula necator, p. 59-66. In S. Heaney, D. Slawson, D. W. Hollomon, M. Smith, P. E. Russels, and D. W. Parry (ed.), Fungicide resistance. British Crop Protection Council, Surrey, United Kingdom.
31. Steva, H., and M. Clerjeau. 1990. Cross resistance to sterol biosynthesis inhibitor fungicides in strains of Uncinula necator isolated in France and Portugal. Med. Fac. Landbouww. Rijksuniv. Gent 55:983-988.
32. Strömstedt, M., D. Rozman, and M. R. Waterman. 1996. The ubiquitously expressed human CYP51 encodes lanosterol 14α-demethylase, a cytochrome P450 whose expression is regulated by oxysterols. Arch. Biochem. Biophys. 329:73-81.
33. Van Nistelrooy, J. G. M., J. M. Van den Brink, J. A. L. Van Kan, R. F. M. Van Gorcom, and M. A. De Waard. 1996. Isolation and molecular characterisation of the gene encoding eburicol 14α-demethylase (CYP51) from Penicillium italicum. Mol. Gen. Genet. 250:725-733.
34. Walmsey-Woodward, D. J., F. A. Laws, and W. J. Whittington. 1979. Studies on the tolerance of Erysiphe graminis f. sp. hordei to systemic fungicides. Ann. Plant Biol. 92:199-209.
35. Yarden, O., and T. Katan. 1993. Mutations leading to substitutions at amino acids 198 and 200 of beta-tubulin that correlate with benomyl-resistance phenotypes of field strains of Botrytis cinerea. Phytopathology 83:1478-1483.
36. Yarwood, C. E. 1978. History and taxonomy of powdery mildews, p. 1-32. In D. M. Spencer (ed.), The powdery mildews. Academic Press, London, United Kingdom.
37. Yoshida, Y. 1993. Lanosterol 14α-demethylase (cytochrome P45014DM), p. 627-639. In H. Schenkman and K. Grein (ed.), Cytochromes P450. Springer-Verlag KG, Berlin, Germany.