Expression, purification, reconstitution and inhibition of Ustilago maydis sterol 14α-demethylase (CYP51; P450(14DM))

FEMS Microbiology Letters

Volumn 169, Issue 2, 1998, Pages 369-373

  Lamb, David C ^a   Kelly, Diane E ^a   Manning, Nigel J ^b   Hollomon, Derek W ^c   Kelly, Steven L ^a  

^a ABERYSTWYTH UNIVERSITY   (United Kingdom)

^b SHEFFIELD CHILDREN S HOSPITAL   (United Kingdom)

^c UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Azole antifungal; Cytochrome P450; Demethylase inhibitor; Purification; Ustilago maydis

Indexed keywords

ANTIFUNGAL AGENT; CYTOCHROME P450; ENZYME INHIBITOR; STEROL;

AGRICULTURE; ARTICLE; CANDIDA ALBICANS; CONTROLLED STUDY; DRUG PURIFICATION; GENE LOCATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; USTILAGO MAYDIS;

AMINO ACID SEQUENCE; AZOLES; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROPHOTOMETRY; TRIAZOLES; USTILAGO;

EID: 0032458367     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00505-9     Document Type: Article

References (17)

1. Hollomon, D.W. (1993) Resistance to azole fungicides in the field. Biochem. Soc. Trans. 21, 1047-1051.
2. Aoyama, Y., Noshiro, M., Gotoh, O., Imaoka, S., Funae, Y., Kurosawa, N., Horiuchi, T. and Yoshida. Y. (1996) Sterol 14-demethylase P450 (P45014DM) is one of the most ancient and conserved P450 species. J. Biochem. 119, 926-933.
3. Yoshida, Y., Noshiro, M., Aoyama, Y., Kawamoto, T., Horiuchi, T. and Gotoh, O. (1998) Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: II. Evolutionary analysis of protein and gene structures. J. Biochem. 122, 1122-1128.
4. Vanden Bossche, H., Willemsens, G., Cools, W., Marichal, P. and Lauwers, W. (1983) Hypothesis on the molecular basis of the antifungal activity of N-substituted imidazoles and triazoles. Biochem. Soc. Trans. 11, 665-667.
5. Kelly, S.L., Lamb, D.C., Kelly, D.E., Loeffler, J. and Einsele, H. (1996) Resistance to fluconazole in Candida albicans from AIDS patients involving cross-resistance to amphotericin. Lancet 348, 1523-1524.
6. 5,6-desaturase as a cause of azole resistance in Ustilago maydis. FEMS Lett. 127, 29-34.
7. 14DM purified from Saccharomyces cerevisiae microsomes. Biochem. Pharmacol. 36, 229-235.
8. Vanden Bossche, H., Marichal, P., Gorrens, J., Bellens, D., Verhoeven, H.D., Coene, M.C., Lauwers, W.F. and Janssen, P.A.J.. (1987) Interaction of azole derivatives with cytochrome P450 isozymes in yeast, fungi and mammalian cells. Pestic. Sci. 21, 289-306.
9. Hargreaves, J.A. and Keon, J.P.R. (1996) Isolation of an Ustilago maydis ERG11 gene and its expression in a mutant deficient in sterol 14α-demethylase activity. FEMS Lett. 139, 203-207.
10. Shyadehi, A.Z., Lamb, D.C., Kelly, S.L., Kelly, D.E., Schunck, W.-H., Wright, J.N., Corina, D. and Akhtar, M. (1996) The mechanism of the acyl-carbon bond cleavage reaction catalysed by recombinant sterol 14α-demethylase of Candida albicans. J. Biol. Chem. 271, 12445-12450.
11. Omura, T. and Sato, R. (1964) The carbon monoxide binding pigment of liver microsomes. I. Evidence for its haemoprotein nature. J. Biol. Chem. 239, 2370-2378.
12. Wiggins, T.E. and Baldwin, B.C. (1984) Interaction of azole derivatives with cytochrome P450 isozymes in yeast, fungi and mammalian cells. Pestic. Sci. 15, 206-209.
13. Ponnamperuma, K. and Croteau, R. (1996) Purification and characterisation of an NADPH cytochrome P450 (cytochrome c) reductase from spearmint (Mentha-spicata) glandular trichomes. Arch. Biochem. Biophys. 329, 9-16.
14. Yoshida, Y. and Aoyama, Y. (1984) Yeast cytochrome P450 catalysing lanosterol 14α-demethylation. I. Purification and spectral properties. J. Biol. Chem. 259, 1655-1660.
15. Hitchcock, C.A., Dickinson, K., Brown, S.B., Evans, E.G.V. and Adams, D.J. (1989) Purification and properties of cytochrome P450 dependent 14α-demethylase from Candida albicans. Biochem. J. 263, 573-579.
16. Lamb, D.C., Kelly, D.E., Baldwin, B.C., Gozzo, F., Boscott, P., Richards, W.G. and Kelly, S.L. (1997) Differential inhibition of Candida albicans CYP51 by stereoisomers of azole antifungals. FEMS Lett. 149, 25-30.
17. Hasemann, C.A., Kurumbail, R.G., Boddupalli, S.S., Peterson, J.A. and Deisenhofer, H. (1995) Structure and function of cytochrome P450: a comparative analysis of three crystal structures. Structure 3, 41-62.