Increased resistance to 14α-demethylase inhibitors (DMIs) in Aspergillus niger by coexpression of the Penicillium italicum eburicol 14α-demethylase (cyp51) and the A. Niger cytochrome P450 reductase (cprA) genes

Journal of Biotechnology

Volumn 49, Issue 1-3, 1996, Pages 13-18

  Van Den Brink, Hans J M ^a   Van Nistelrooy, Hans J G M ^b   De Waard, Maarten A ^b   Van Den Hondel, Cees A M J J ^a   Van Gorcom, Robert F M ^a  

^a TNO   (Netherlands)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Ergosterol; Fungi; NADPH cytochrome P450 reductase; Sterol 14 demethylation inhibitors

Indexed keywords

DRUG PRODUCTS; ENZYME INHIBITION; FUNGICIDES; GENES;

ASPERGILLUS NIGER; DEMETHYLASE INHIBITORS (DMI); GENE ENCODING; PENICILLIUM ITALICUM;

GENETIC ENGINEERING;

CYTOCHROME P450 REDUCTASE; ENZYME INHIBITOR; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

ARTICLE; ASPERGILLUS NIGER; ENZYME SYNTHESIS; FUNGAL GENETICS; NONHUMAN; PENICILLIUM; PRIORITY JOURNAL;

ASPERGILLUS NIGER; FUNGI; PENICILLIUM; PENICILLIUM ITALICUM;

EID: 0030595053     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01403-4     Document Type: Article

References (22)

1. Ballard, S.A., Ellis, S.W., Kelly, S.L. and Troke, P.F. (1990) A novel method for studying ergosterol biosynthesis by a cell free preparation of Aspergillus fumigatus and its inhibition by azole antifungal agents. J. Med. Vet. Mycol. 28, 335-344.
2. Bos, C.J. (1986) Induced mutation and somatic recombination as tools for genetic analysis and breeding of imperfect fungi. Thesis. Agricultural University, Wageningen, the Netherlands.
3. Gonzalez, F.J. and Korzekwa, K.R. (1995) Cytochrome P450 expression systems. Annu. Rev. Pharmacol. Toxicol. 35, 369-390.
4. Kalb, V.F., Loper, J.C., Dey, C.R., Woods, C.W. and Sutter, T.R. (1986) Isolation of a cytochrome P450 structural gene from Saccharomyces cerevisiae. Gene 45, 237-245.
5. Kalb, V.F., Woods, C.W., Turi, T.G., Dey, C.R., Sutter, T.R. and Loper, J.C. (1987) Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae. DNA 6, 529-537.
6. Kapteyn, J.C., Pillmoor, J.B. and de Waard, M.A. (1993) Biochemical mechanisms involved in the selective fungitoxicity of two eburicol 14α-demethylation inhibitors, procloraz and an quinconazole: accumulation and metabolism studies. Pestic. Sci. 36, 85-93.
7. Pontecorvo, G., Roper, J.A., Hemmons, L.J., MacDonald, K.D. and Bufton, A.W.J. (1953) The genetics of Aspergillus nidulans. Adv. Genet. 5, 141-238.
8. Punt, P.J., Oliver, R.P., Dingemanse, M.A., Pouwels, P.H. and van den Hondel, C.A.M.J.J. (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56, 117-124.
9. Punt, P.J., Dingemanse, M.A., Jacobs-Meijsing, B.J.M., Pouwels, P.H. and van den Hondel, C.A.M.J.J. (1988) Isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulans. Gene 69, 49-57.
10. Punt, P.J. and van den Hondel. C.A.M.J.J. (1993) Transformation of filamentous fungi based on hygromycin B and phleomycine resistance markers. Methods Enzymol. 216, 447-457.
11. Sakaki, T., Shibata, M., Yabusaki, Y., Murakami, H. and Ohkawa, H. (1990) Expression of bovine cytochrome P450c21 and its fused enzymes with yeast NADPH-cytochrome P450 reductase in Saccharomyces cerevisiae. DNA Cell Biol. 9(8), 603-614.
12. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Stehmann, C., Kapteyn, J.C. and de Waard, M.A. (1994) Development of a cell-free assay from Botrytis cinerea as a biochemically screen for sterol biosynthesis inhibitors. Pestic. Sci. 40, 1-8.
14. Truan, G., Cullin, C., Reisdorf, P., Urban, P. and Pompon, D. (1993) Enhanced in vivo monooxygenase activities of mammalian P450s in engineered yeast cells producing high levels of NADPH-P450 reductase and human cytochrome b5. Gene 125, 49-55.
15. Vanden Bossche, H. (1988) Mode of action of pyridine, pyrimidine and azole antifungals. In: Berg, D. and Plempel, M. (Eds.), Sterol Biosynthesis inhibitors, Pharmaceutical and Agrochemical aspects. Ellis Horwood, Chichester, UK, pp. 79-119.
16. van den Brink, J.M., van Zeijl, C.M.J., van den Hondel, C.A.M.J.J. and van Gorcom, R.F.M. (1995) Cloning and characterization of the NADPH-cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger. DNA Cell Biol. 14, 719-729.
17. van Nistelrooy, J.G.M., van den Brink, J.M., van Kan, J.A.L., van Gorcom, R.F.M. and de Waard, M.A. (1996) Isolation and molecular characterization of the gene encoding eburicol 14α-demethylase from Penicillium italicum. Mol. Gen. Genet., 250, 725-733.
18. Verdoes, J.C., Punt, P.J., Schrickx, J.M., van Verseveld, H.W., Stouthamer, A.H. and van den Hondel, C.A.M.J.J. (1993) Glucoamylase overexpression in Aspergillus niger: molecular genetic analysis of strains containing multiple copies of the glaA gene. Transgen, Res. 2, 84-92.
19. Verdoes, J.C., Calil, M.R., Punt, P.J., Debets, F., Swart, K., Stouthamer, A.H. and van den Hondel, C.A.M.J.J. (1994) The complete karyotype of Aspergillus niger: the use of introduced electrophoretic mobility variation of chromosomes for gene assignment studies. Mol. Gen. Genet. 224, 75-80.
20. Weber, J.M., Reiser, J. and Kaeppeli, O. (1990) Lanosterol 14α-demethylase-encoding gene: systematic analysis of homologous overexpression in Saccharomyces cerevisiae using strong yeast promoters. Gene 87, 167-175.
21. Weber, J.M., Ponti, C.G., Kaeppeli, O. and Reiser, J. (1992) Factors affecting homologous overexpression of the Saccharomyces cerevisiae lanosterol 14α-demethylase gene. Yeast 8, 519-533.
22. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13mp18 and pUC19 vectors. Gene 33, 103-119.