Engineering subtilisin E for enhanced stability and activity in polar organic solvents

Journal of Biochemistry

Volumn 127, Issue 4, 2000, Pages 617-625

  Takagi, Hiroshi ^a   Hirai, Kazunori ^a   Maeda, Yoshimi ^b   Matsuzawa, Hiroshi ^c,d   Nakamori, Shigeru ^a  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

^b IWAKI MEISEI UNIVERSITY   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d AOMORI UNIVERSITY   (Japan)

Author keywords

Aqualysin I; Disulfide bond; Site directed mutagenesis; Stability in organic solvents; Subtilisin E

Indexed keywords

AQUALYSIN; MUTANT PROTEIN; N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT; SERINE PROTEINASE; SUBTILISIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; BINDING SITE; DISULFIDE BOND; ENZYME SYNTHESIS; GENETIC ENGINEERING; MOLECULAR MODEL; NONHUMAN; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); FELIS CATUS; NEGIBACTERIA;

EID: 0034050369     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022649     Document Type: Article

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