Random mutagenesis into the conserved Gly154 of subtilisin E: Isolation and characterization of the revertant enzymes

Protein Engineering

Volumn 11, Issue 12, 1998, Pages 1205-1210

  Takagi, Hiroshi ^a   Yamamoto, Manami ^a   Ohtsu, Iwao ^a   Nakamori, Shigeru ^a  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Catalytic efficiency; Random mutagenesis; Substrate specificity; Subtilisin E

Indexed keywords

PROTEINASE; SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; ENZYME ISOLATION; ENZYME MECHANISM; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; MUTAGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 0032442236     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/11.12.1205     Document Type: Article

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