Restriction of substrate specificity of subtilisin E by introduction of a side chain into a conserved glycine residue

FEBS Letters

Volumn 395, Issue 2-3, 1996, Pages 127-132

  Takagi, Hiroshi ^a   Maeda, Tadao ^a   Ohtsu, Iwao ^a   Tsai, Ying Chieh ^b   Nakamori, Shigeru ^a  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

^b NATIONAL YANG MING UNIVERSITY   (Taiwan)

Author keywords

Site directed mutagenesis; Substrate specificity; Subtilisin

Indexed keywords

SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CRYSTALLOGRAPHY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; MOLECULAR MODEL; MUTANT; PRIORITY JOURNAL;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 0030597168     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/0014-5793(96)01014-9     Document Type: Article

References (29)

1. [1] Craik, C.S., Largman, C., Fletcher, T., Roczniak, S., Barr, P.J., Fletterick, R. and Rutter, W.J. (1985) Science 228, 291-297.
2. [2] Wilks, H.M., Hart, K.W., Feeney, R., Dunn, C.R., Muirhead, H., Chia, W.N., Barstow, D.A., Atkinson, T., Clarke, A.R. and Holbrook, J.J. (1988) Science 242, 1541-1544.
3. [3] Bone, R., Silen, J.L. and Agard, D.A. (1989) Nature 339, 191-195.
4. [4] Wells, J.A. and Estell, D.A. (1988) Trends Biochem. Sci. 13, 291-297.
5. [5] Takagi, H. (1993) Int. J. Biochem. 25, 307-312.
6. [6] Wright, C.S., Alden, R.A. and Kraut, J. (1969) Nature 221, 235-242.
7. [7] Wells, J.A., Cunningham, B.C., Graycar, T.P. and Estell, D.A. (1987) Proc. Natl. Acad. Sci. USA 84, 5167-5171.
8. [8] Estell, D.A., Graycar, T.P., Miller, J.V., Powers, D.B., Burnier, J.P., Ng, P.G. and Wells, J.A. (1986) Science 233, 659-663.
9. [9] Wells, J.A., Powers, D.B., Bott, R.R., Graycar, T.P. and Estell, D.A. (1987) Proc. Natl. Acad. Sci. USA 84, 1219-1223.
10. [10] Carter, P., Nilsson, B., Burnier, J.P., Burdick, D. and Wells, J.A. (1989) Proteins: Struct. Funct. Genet. 6, 240-248.
11. [11] Siezen, R.J., De Vos, W.M., Leunissen, J.A.M. and Dijkstra, B.W. (1991) Protein Eng. 7, 719-737.
12. [12] Ikemura, H., Takagi, H. and Inouye, M. (1987) J. Biol. Chem. 262, 7859-7864.
13. [13] Takagi, H., Morinaga, Y., Tsuchiya, M., Ikemura, H. and Inouye, M. (1988) Bio/Technology 6, 948-950.
14. [14] Takagi, H., Arafuka, S., Inouoye, M. and Yamasaki, M. (1992) J. Biochem. 111, 584-588.
15. [15] Nakamura, K., Masui, Y. and Inouye, M. (1982) J. Mol. Appl. Genet. 1, 289-299.
16. [16] Ghyayeb, J., Kimura, H., Takahara, M., Hsiung, H., Masui, Y. and Inouye, M. (1984) EMBO J. 3, 2437-2442.
17. [17] Shinha, N.D., Biernat, J., McManus, J. and Koster, H. (1984) Nucleic Acids Res. 12, 4539-4557.
18. [18] Inouye, M. and Inouye, S. (1987) in: Synthesis of DNA, RNA and Their Application (Narang, S. ed.) pp. 181-206, Academic Press, New York.
19. [19] Sanger, F., Coulson, A.R., Barrell, B.G., Smith, A.J.H. and Roe, B.A. (1980) J. Mol. Biol. 143, 161-178.
20. [20] Takagi, H., Morinaga, Y., Ikemura, H. and Inouye, M. (1988) J. Biol. Chem. 263, 19592-19596.
21. [21] Miller, J.H. (1972) in: Experiments in Molecular Genetics (Jeffrey, H.M. ed.) pp. 431-432, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
22. [22] Takagi, H., Morinaga, Y., Ikemura, H. and Inouye, M. (1989) J. Biochem. 105, 953-956.
23. [23] Koshland, D. and Botstein, D. (1980) Cell 20, 749-760.
24. [24] Hagihara, B., Matsubara, H., Nakai, M. and Okunuki, K. (1958) J. Biochem. 45, 185-194.
25. [25] Chu, N.-M., Chao, Y. and Bi, R.-C. (1995) Protein Eng. 8, 211-215.
26. [26] Perona, J.J., Hedstrom, L., Rutter, W.J. and Fletterick, R.J. (1995) Biochemistry 34, 1489-1499.
27. [27] Navia, M.A., McKeever, B.M., Springer, J.P., Lin, T.-Y., Williams, H.R., Fluder, E.M., Dorn, C.P. and Hoogsteen, K. (1989) Proc. Natl. Acad. Sci. USA 86, 7-11.
28. [28] Fox, T., Mason, P., Storer, A.C. and Mort, J.S. (1995) Protein Eng. 8, 53-57.
29. [29] Matthews, D.A., Alden, R.A., Birktoft, J.J., Freer, S.T. and Kraut, J. (1977) J. Biol. Chem. 252, 8875-8883.