Reticulocyte cellular indices: A new approach in the diagnosis of anemias and monitoring of erythropoietic function

Critical Reviews in Clinical Laboratory Sciences

Volumn 37, Issue 2, 2000, Pages 93-130

  Brugnara, Carlo ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Automated hematology; CHr; Immature reticulocyte fraction; MCH.; MCHC; MCV; Reticulocytes

Indexed keywords

HEMOGLOBIN; IRON; MESSENGER RNA; RECOMBINANT ERYTHROPOIETIN;

ANEMIA; BIOCHEMISTRY; BONE MARROW TRANSPLANTATION; CELL MATURATION; CELL VOLUME; ERYTHROPOIESIS; FLUORESCENCE; HEALTH CARE COST; HEALTH CARE QUALITY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMOGLOBIN DETERMINATION; HUMAN; IRON DEFICIENCY; IRON METABOLISM; LEUKOCYTE COUNT; MEGALOBLASTIC ANEMIA; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; RETICULOCYTE; REVIEW;

EID: 0034016982     PISSN: 10408363     EISSN: None     Source Type: Journal    
DOI: 10.1080/10408360091174196     Document Type: Review

References (168)

1. 1. National Committee for Clinical Laboratory Standards (NCCLS). Methods for reticulocyte counting; proposed standards. Villanova, PA; 1985.
2. 2. International Council for Standardization in Hematology (ICSH). ICSH guidelines for reticulocyte counting by microscopy on supravitally stained samples. WHO/LBS 1992; 92: 3.
3. 3. Heilmeyer L. Blutfarbstoffwechselstudien. Dtsch Arch Klin Med 1931; 171: 123-125.
4. 4. Heilmeyer L, Westhauser R. Reifungsstudien an uberlebenden Reticulocyten in vitro. Zeitschr Klin Med 1932; 121: 361-379.
5. 5. Lowenstein LM. The mammalian reticulocyte. Int Rev Cytol 1959; 8: 135-174.
6. 6. Gilmer PR , Koepke JA. The reticulocyte: am approach to definition. Am J Clin Pathol 1976; 66: 262-267.
7. 7. Perrotta AL. The polychromatophilic erythrocyte. Am J Clin Pathol 1972; 57: 471-477.
8. 8. Finch CA. Some quantitative aspects of erythropoiesis. Ann NY Acad Sci USA 1959; 77: 410-416.
9. 9. Clarkson DR, Moore EM. Reticulocyte size in nutritional anemias. Blood 1976; 48: 669-677.
10. 10. Song SH, Groom AC. Sequestration and possible maturation of reticulocytes in the normal spleen. Can J Physiol Pharmacol 1972; 50: 400-406.
11. 11. Hillman RS. Characteristics of marrow production and reticulocyte maturation in normal man in response to anemia. J Clin Invest 1969; 48: 443-453.
12. 12. Come SE, Shohet SB Robinson SH. Surface remodeling vs. whole cell hemolysis of reticulocytes produced with erythroid stimulation or iron deficiency anemia. Blood 1974; 44: 817-830.
13. 13. Robinson SH, Tsong M. Hemolysis of "stress" reticulocytes: a source of erythropoietic bilirubin formation. J Clin Invest 1970; 49: 1025-1034.
14. 14. Coulombel L, Tchernia G Mohandas N. Human reticulocytes' maturation and its relevance to erythropoietic stress. J Lab Clin Med 1979;94: 467-474.
15. 15. Chasis JA, Prenant M, Leung A , et al. Membrane assembly and remodeling during reticulocyte maturation. Blood 1989; 74: 1112-1120.
16. 16. Shimada A. The maturation of reticulocytes. I. Following introduction of reticulocytes into polycythemic and normocythemic animals. Acta Med Okayama 1975; 29: 273-282.
17. 17. Shimada A. The maturation of reticulocytes II. Life-span of red cells originating from stress reticulocytes. Acta Med Okayama 1975; 29: 283-289.
18. 18. Stohlman F, Jr. Humoral regulation of erythropoiesis. VII. Shortened survival of erythrocytes produced by erythropoietine or severe anemia. Proc Soc Exp Biol Med 1961; 107: 884-887.
19. 19. Blau AC, Constantoulakis P, Al-Khatti A, et al. Fetal hemoglobin in acute and chronic states of erythroid expansion. Blood 1993; 81: 227-233.
20. 20. Nagel RL, Vichinsky E, Shah M, et al. Reticulocyte response in sickle cell anemia treated with recombinant human erythropoietin: a double-blind study. Blood 1993; 81: 9-14.
21. 21. Charache S, Terrin ML, Moore RD, et al. Effect of hydroxyurea on the frequency of painful crises in sickle cell anemia. Investigators of the Multicenter Study of Hydroxyurea in Sickle Cell Anemia. N Engl J Med 1995; 332: 1317-22.
22. 22. de Montalembert M, Belloy M, Bernaudin F, et al. Three-year follow-up of hydroxyurea treatment in severely ill children with sickle cell disease. The French Study Group on Sickle Cell Disease. J Pediatr Hematol Oncol 1997; 19: 313-8.
23. 23. Bunn HF. Pathogenesis and treatment of sickle cell disease. N Engl J Med 1997; 337: 762-9.
24. 24. Eaton WA, Hofrichter J. The biophysics of sickle cell hydroxyurea therapy. Science 1995; 268: 1142-3.
25. 25. Platt OS. Sickle cell paths converge on hydroxyurea. Nature Med 1995; 1: 307-8.
26. 26. Franco RS, Barker-Gear R, Miller MA, et al. Fetal hemoglobin and potassium in isolated transferrin receptor-positive dense sickle reticulocytes. Blood 1994; 84: 2013-20.
27. 27. Franco RS, Palascak M, Thompson H , et al. KC1 cotransport activity in light versus dense transferrin receptor-positive sickle reticulocytes. J Clin Invest 1995; 95: 2573-80.
28. 28. Franco RS, Palascak M, Thompson H, et al. Dehydration of transferrin receptor-positive sickle reticulocytes during continuous or cyclic deoxygenation: role of KC1 cotransport and extracellular calcium. Blood 1996; 88: 4359-65.
29. 29. Franco RS, Thompson H, Palascak M, et al. The formation of transferrin receptor-positive sickle reticulocytes with intermediate density is not determined by fetal hemoglobin content. Blood 1997; 90: 3195-203.
30. 30. Paolino W. II comportamento dei diametri delle classi reticolocitarie ed il suo significato nella fisiopatologia della eritropoiesi. Minerva Med 1946; 18: 327-333.
31. 31. Killman SA. On the size of normal human reticulocytes. Acta Med Scand 1964; 176:529-533.
32. 32. d'Onofrio G, Chirillo R, Zini G, et al. Simultaneous measurement of reticulocyte and red blood cell indices in healthy subjects and patients with microcytic and macrocytic anemia. Blood 1995; 85: 818-823.
33. 33. Buttarello M, Bulian P, Venudo A , et al. Laboratory evaluation of the Miles H*3 automated reticulocyte counter. A comparative study with manual reference method and Sysmex R-1000. Arch Pathol Lab Med 1995; 119: 1141-1148.
34. 34. Betke K, Rodig I. Zur Frage des Volumens der vital-granulierten Erythrocyten (Retyculocyten). Klin Wochenschr 1955; 33: 911-912.
35. 35. Weicker H, Fichser H. Das Reticulocytenvolumen. Klin Wochenschr 1955; 33: 1074-1082.
36. 36. Brecher G, Stohlman F. Reticulocyte size and erythropoietic stimulation. Proc Soc Exp Biol Med 1961; 107: 887-891.
37. 37. Noble NA, Xu QP Ward JH. Reticulocytes. I. Isolation and in vitro maturation of synchronized populations. Blood 1989: 475-481.
38. 38. Noble NA, Xu QP Hoge LL. Reticulocytes. II. Reexamination of the in vivo stress reticulocytes. Blood 1990; 75: 1887-1892.
39. 39. Brecher G , Stohlman F. The macrocytic response to erythropoietin stimulation. In: LO Jacobson and M Doyle, eds. Erythropoiesis. Pp. 216-224. New York: Grune and Stratton, 1962.
40. 40. Bessman D. Erythropoiesis during recovery from iron deficiency: normocytes and macrocytes. Blood 1977; 50: 987-993.
41. 41. Crouch JY , Kaplow LS. Relationship of reticulocyte age to polychromasia, shift cells, and shift reticulocytes. Arch Pathol Lab Med 1985; 109: 325-329.
42. 42. Weiser MG, Kociba GJ. Persistent macrocytosis assessed by erythrocyte subpopulation analysis following erythrocyte regeneration in cats. Blood 1982; 60: 295-303.
43. 43. Bosch FH, Werre JM, Roerdinkholder-Stoelwinder B, et al. Young red blood cells; observations about the cytometry and morphologic alterations in the beginning of their life. Clin Lab Haematol 1993; 15: 265-274.
44. 44. Sorette MP, Shiffer K Clark MR. Improved isolation of normal human reticulocytes via exploitation of chloride-dependent potassium transport. Blood 1992; 80: 249-254.
45. 45. Bain BJ, Cavill IA. Hypochromic macrocytes: are they reticulocytes? J Clin Pathol 1993; 46: 963-4.
46. 46. Brugnara C, Hipp MJ, Irving PJ, et al. Automated reticulocyte counting and measurement of reticulocyte cellular indices: evaluation of the Miles H*3 blood analyzer. Am J Clin Pathol 1994; 102: 623-632.
47. 47. Schwartz RS, Musto S, Fabry ME et al. Two distinct pathways mediate the formation of intermediate density cells and hyperdense cells from normal density sickle red blood cells. Blood 1998; 92: 4844-4855.
48. 48. Franco RS, Lohmann J, Silberstein EB, et al. Time-dependent changes in the density and hemoglobin F content of biotin-labeled sickle cells. J Clin Invest 1998; 101: 2730-40.
49. 49. Lee LG, Ciu LA. Thiazole orange: a new dye for reticulocyte analysis. Cytometry 1986; 7: 508-517.
50. 50. Davis BH, Bigelow NC. Flow cytometric reticulocyte quantification using thiazole orange provides clinically useful reticulocyte maturity index. Arch Pathol Lab Med 1989; 113: 684-689.
51. 51. Ferguson DJ, Lee SF Gordon PA. Evaluation of reticulocyte counts by flow cytometry in a routine laboratory. Am J Hematol 1990; 33: 13-17.
52. 52. Schimenti KJ, Lacerna K, Wamble A, et al. Reticulocyte quantification by flow cytometry, image analysis and manual counting. Cytometry 1992; 13: 853-862.
53. 53. Tichelli A, Gratwohl A, Driessen A, et al. Evaluation of the Sysmex R-1000". An automated reticulocyte analyzer. Am J Clin Pathol 1990; 93: 70-78.
54. 54. Cavill I. The rejected reticulocyte. Br J Haematol 1993; 84: 563-565.
55. 55. d'Onofrio G, Kim YR, Schulze S, et al. Evaluation of the Abbott Cell Dyn 4000 automated fluorescent reticulocyte measurements: comparison with manual, FACScan and Sysmex R1000 methods. Clin Lab Haematol 1997; 19: 253-60.
56. 56. NCFCLS (NCCLS). Reticulocyte counting by flow cytometry; proposed guideline. Villanova, PA; 1993: NCCLS document H44-P.
57. 57. Savage RA, Skoog DP Rabinovitch A. Analytic inaccuracy and imprecision in reticulocyte counting: a preliminary report from the College of American Pathologists reticulocyte project. Blood Cells 1985; 11: 97-112.
58. 58. Davis BH, Bigelow NC, Koepke JA, et al. Interlaboratory comparison of flow cytometric reticulocyte analysis. Blood 1992; 80: 280a.
59. 59. Kim YR, Kantor J, Landayan M, et al. A rapid and sensitive reticulocyte method on a high-throughput hematology instrument. Lab Hematol 1997; 3: 19-26.
60. 60. Lofsness KG, Kohnke ML, Geier NA. Evaluation of automated reticulocyte counts and their reliability in the presence of Howell-Jolly bodies. Am J Clin Pathol 1994; 101: 85-90.
61. 61. Ghevaert C, Fournier M, Reade V, et al. Laboratory evaluation of the Coulter STKS reticulocyte method in a children's hospital. Lab Hematol 1997; 3: 92-97.
62. 62. Buttarello M, Bulian P, De Pra M, et al. Reticulocyte quantification by Coulter MAXM VCS (volume, conductivity, light scatter) technology. Clin Chem 1996; 42: 1930-1937.
63. 63. Kessler C, Machin SJ, Pollard Y, et al. Reticulocyte performance on the Coulter® Gen-S™ system. Lab Hematol 1997; 3: 41-47.
64. 64. Tycko DH, Metz M H, Epstein E A, et al. A flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration. J Appl Optics 1985; 24: 1355-1365.
65. 65. Fossat G, David M, Harle J R, et al. New parameters in erythrocyte counting. Arch Pathol Lab Med 1987; 111: 1150-1154.
66. 66. Mohandas N, Kim YR, Tycko DH, et al. Accurate and independent measurement of volume and hemoglobin concentration of individual red cells by laser light scattering. Blood 1986; 68: 506-513.
67. 67. Mohandas N, Johnson A, Wyatt J, et al. Automated quantitation of cell density distribution and hyperdense cell fraction in RBC disorders. Blood 1989; 74: 442-447.
68. 68. Sandberg S, Rustad P, Johannesen B, et al. Within-subject biological variation of reticulocytes and reticulocyte-derived parameters. Eur J Haematol 1998; 61: 42-8.
69. 69. Bridges KR, Barabino GD, Brugnara C, et al. A multiparameter analysis of sickle erythrocytes in patients undergoing hydroxyurea therapy. Blood 1996; 88: 4701-10.
70. 70. Brugnara C, Zelmanovic D, Sorette M, et al. Reticulocyte Hemoglobin: an integrated parameter for evaluation of erythropoietic activity. Am J Clin Pathol 1997; 108: 133-142.
71. 71. Major A, Mathez-Loic F, Gautschi K, et al. The effect of intravenous iron on the reticulocyte response to recombinant human erythropoietin. Br J Haematol 1997; 98: 292-294.
72. 72. Colella GM, Fan S Mohandas N. Changes in cell volume, hemoglobin content, and hemoglobin concentration during maturation of normal human reticulocytes. Blood 1991; 78: 407a (abstr).
73. 14C in reticulocytes. Biochim Biophys Acta 1954; 13: 583-586.
74. 74. Tchernia G. Neonatal blood. In: Brugnara C and Von Kapff CT, eds. Bayer international atlas of hematology. Pp. 96-99. Tarrytown, NY: Bayer Corporation; 1996.
75. 75. Diagne I, Archambeaud MP, Diallo D, et al. Erythrocyte indices and iron stores in cord blood. Arch Pediatr 1995; 2: 208-14.
76. 76. Davis BH, Bigelow N, Ball ED, et al. Utility of flow cytometric reticulocyte quantification as a predictor of engraftment in autologous bone marrow transplantation. Am J Hematol 1989; 32: 81-87.
77. 77. Davis BH, DiCorato M, Bigelow NC, et al. Proposal for standardization of flow cytometric reticulocyte maturity index (RMI) measurements. Cytometry 1993; 14: 318-326.
78. 78. Davis BH. Immature reticulocyte fraction (IRF): by any name, a useful clinical parameter of erythropoietic activity. Lab Hematol 1996; 2: 2-8.
79. 79. Davis BH, Bigelow NC van Hove L. Immature reticulocyte fraction (IRF) and reticulocyte counts: comparison of CELL-DYN® 4000, Sysmex R-3000, thiazole flow cytometry, and manual counts. Lab Hematol 1996; 2: 144-150.
80. 80. Davis BH. Report on the ISLH-sponsored immature reticulocyte fraction (IRF) workshop. Lab Hematol 1997; 3: 261-263.
81. 81. Davis BH, Bigelow NC, Koepke JA, et al. Flow cytometric reticulocyte analysis. Multiinstitutional interlaboratory correlation study. Am J Clin Pathol 1994; 102: 468-477.
82. 82. Villamor N, Kirsch A, Huhn D, et al. Interference of blood leucocytes in the measurements of immature red cells (reticulocytes) by two different (semi-) automated flow-cytometry technologies. Clin Lab Haematol 1996; 18: 89-94.
83. 83. Conrad ME , Crosby WH. The natural history of iron deficiency induced by phlebotomy. Blood 1962; 20: 173-185.
84. 84. Herbert V. Experimental nutritional folate deficiency in man. Trans Assoc Am Physicians 1962; 73: 307-320.
85. 85. Green R. Disorders of inadequate iron. Hosp Pract 1991; 26S: 25-29.
86. 86. Brugnara C, Laufer MR, Friedman AJ, et al. Reticulocyte hemoglobin content (CHr): early indicator of iron deficiency and response to therapy. Blood 1994; 83: 3100 (letter).
87. 87. Eschbach JW, Egrie JC, Downing MR, et al. Correction of the anemia of end-stage renal disease with recombinant human erythropoietin. Results of a combined phase 1 and II clinical trial. N Engl J Med 1987; 316: 73-8.
88. 88. Eschbach JW, Kelly MR, Haley NR, et al. Treatment of the anemia of progressive renal failure with recombinant human erythropoietin. N Engl J Med 1989; 321: 158-63.
89. 89. Macdougall IC. How to get the best out of r-HuEPO. Nephrol Dial Transplant 1995; 10: 85-91.
90. 90. Kaufman JS, Reda DJ, Fye CL, et al. Subcutaneous compared with intravenous epoetin in patients receiving hemodialysis. Department of Veterans Affairs Cooperative Study Group on Erythropoietin in Hemodialysis Patients. N Engl J Med 1998; 339: 578-83.
91. 91. Macdougall IC. Poor response to erythropoietin: practical guidelines on investigation and management. Nephrol Dial Transplant 1995; 10: 607-14.
92. 92. Macdougall IC. Treatment of renal anemia with recombinant human erythropoietin. Curr Opin Nephrol Hypertens 1992; 1: 210-9.
93. 93. Macdougall IC. Meeting the challenges of a new millennium: optimizing the use of recombinant human erythropoietin. Nephrol Dial Transplant 1998; 13: 23-7.
94. 94. Rao DS, Shih MS Mohini R. Effect of serum parathyroid hormone and bone marrow fibrosis on the response to erythropoietin in uremia. N Engl J Med 1993; 328: 171-5.
95. 95. Koury MJ. Investigating erythropoietin resistance. N Engl J Med 1993; 328: 205-6.
96. 96. Ifudu O, Feldman J Friedman EA. The intensity of hemodialysis and the response to erythropoietin in patients with end-stage renal disease. N Engl J Med 1996; 334: 420-5.
97. 97. Lacombe C. Resistance to erythropoietin. N Engl J Med 1996; 334: 660-2.
98. 98. Madore F, Lowrie EG, Brugnara C, et al. Anemia in hemodialysis patients: variables affecting this outcome predictor. J Am Soc Nephrol 1997; 8: 1921-9.
99. 99. Avram MM, Mittman N, Bonomini L, et al. Markers for survival in dialysis: a seven-year prospective study. Am J Kidney Dis 1995; 26: 209-19.
100. 100. Besarab A, Bolton WK, Browne JK, et al. The effects of normal as compared with low hematocrit values in patients with cardiac disease who are receiving hemodialysis and epoetin. N Engl J Med 1998; 339: 584-90.
101. 101. Horl WH. How to get the best out of r-HuEPO. Nephrol Dial Transplant 1995; 10: 92-5.
102. 102. Horl WH, Cavill I, MacDougall IC, et al. How to diagnose and correct iron deficiency during r-huEPO therapy - a consensus report. Nephrol Dial Transplant 1996;11:246-50.
103. 103. Fishbane S, Kowalski EA, Imbriano LJ, et al. The evaluation of iron status in hemodialysis patients. J Am Soc Nephrol 1996; 7: 2654-7.
104. 104. Fishbane S, Maesaka JK. Iron management in end-stage renal disease. Am J Kidney Dis 1997; 29: 319-33.
105. 105. Drueke TB, Barany P, Cazzola M, et al. Management of iron deficiency in renal anemia: guidelines for the optimal therapeutic approach in erythropoietin-treated patients. Clin Nephrol 1997; 48: 1-8.
106. 106. Fishbane S, Frei GL Maesaka J. Reduction in recombinant human erythropoietin doses by the use of chronic intravenous iron supplementation. Am J Kidney Dis 1995; 26: 41-6.
107. 107. Fishbane S, Lynn RI. The efficacy of iron dextran for the treatment of iron deficiency in hemodialysis patients. Clin Nephrol 1995; 44: 238-40.
108. 108. Fishbane S, Ungureanu VD, Maesaka JK, et al. The safety of intravenous iron dextran in hemodialysis patients. Am J Kidney Dis 1996; 28: 529-34.
109. 109. Macdougall IC, Cavill I, Hulme B, et al. Detection of functional iron deficiency during erythropoietin treatment: a new approach. Br Med J 1992; 304: 225-226.
110. 110. Macdougall IC. Monitoring of iron status and iron supplementation in patients treated with erythropoietin. Curr Opin Nephrol Hypertens 1994; 3: 620-5.
111. 111. Macdougall IC. What is the most appropriate strategy to monitor functional iron deficiency in the dialysed patient on rhEPO therapy? Merits of percentage hypochromic red cells as a marker of functional iron deficiency. Nephrol Dial Transplant 1998; 13: 847-9.
112. 112. Schaefer RM, Schaefer L. Iron monitoring and supplementation: how do we achieve the best results? Nephrol Dial Transplant 1998; 13: 9-12.
113. 113. Braun J, Lindner K, Schreiber M, et al. Percentage of hypochromic red blood cells as predictor of erythropoietic and iron response after i.v. iron supplementation in maintenance haemodialysis patients. Nephrol Dial Transplant 1997; 12: 1173-81.
114. 114. Sowade O, Messinger D, Franke W, et al. The estimation of efficacy of oral iron supplementation during treatment with epoetin beta (recombinant human erythropoietin) in patients undergoing cardiac surgery. Eur J Haematol 1998; 60: 252-9.
115. 115. Fishbane S, Galgano C, Langley RC, Jr, et al. Reticulocyte hemoglobin content in the evaluation of iron status of hemodialysis patients. Kidney Int 1997; 52: 217-22.
116. 116. Horl WH. What is the most appropriate strategy to monitor functional iron deficiency in the dialysed patient on rhEPO therapy? Measurement of hypochromic red blood cells is not the first line procedure to identify the patient with iron deficiency. Nephrol Dial Transplant 1998; 13: 850-1.
117. 117. Garrett S, Worwood M. Zinc protoporphyrin and iron-deficient erythropoiesis. Acta Haematol 1994; 91: 21-5.
118. 118. Beguin Y, Loo M, R'Zik S, et al. Early prediction of response to recombinant human erythropoietin in patients with the anemia of renal failure by serum transferrin receptor and fibrinogen. Blood 1993; 82: 2010-2016.
119. 119. Mittman N, Sreedhara R, Mushnick R, et al. Reticulocyte hemoglobin content predicts functional iron deficiency in hemodialysis patients receiving rHuEPO. Am J Kidney Dis 1997; 30: 912-22.
120. 120. Bhandari S, Turney JH, Brownjohn AM, et al. Reticulocyte indices in patients with end stage renal disease on hemodialysis. J Nephrol 1998; 11: 78-82.
121. 121. Fischl M, Galpin JE, Levine JD, et al. Recombinant human erythropoietin for patients with AIDS treated with zidovudine. N Engl J Med 1990; 322: 1488-93.
122. 122. Miller CB, Jones RJ, Piantadosi S, et al. Decreased erythropoietin response in patients with the anemia of cancer. N Engl J Med 1990; 322: 1689-92.
123. 123. Goodnough LT, Rudnick S, Price TH, et al. Increased preoperative collection of autologous blood with recombinant human erythropoietin therapy. N Engl J Med 1989; 321: 1163-8.
124. 124. Rutherford CJ, Schneider TJ, Dempsey H, et al. Efficacy of different dosing regimens for recombinant human erythropoietin in a simulated perisurgical setting: the importance of iron availability in optimizing response. Am J Med 1994; 96: 139-45.
125. 125. Brugnara C, Colella GM, Cremins JC, et al. Effects of subcutaneous recombinant human erythropoietin in normal subjects: development of decreased reticulocyte hemoglobin content and iron-deficient erythropoiesis. J Lab Clin Med 1994; 123: 660-667.
126. 126. Maier RF, Obladen M, Scigalla P, et al. The effect of epoetin beta (recombinant human erythropoietin) on the need for transfusion in very-low-birth-weight infants. European Multicentre Erythropoietin Study Group. N Engl J Med 1994; 330: 1173-8.
127. 127. Cazzola M, Mercuriali F, Brugnara C. Use of recombinant human erythropoietin outside the setting of uremia. Blood 1997; 89: 4248-4267.
128. 128. Brugnara C, Chambers LA, Malynn E, et al. Red cell regeneration induced by subcutaneous recombinant erythropoietin: iron-deficient erythropoiesis in iron-replete subjects. Blood 1993; 81: 956-964.
129. 129. Breymann C, Bauer C, Major A, et al. Optimal timing of repeated rh-erythropoietin administration improves its effectiveness in stimulating erythropoiesis in healthy volunteers. Br J Haematol 1996; 93: 295-301.
130. 130. Sowade O, Sowade B, Brilla K, et al. Kinetics of reticulocyte maturity fractions and indices and iron status during therapy with epoetin beta (recombinant human erythropoietin) in cardiac surgery patients. Am J Hematol 1997; 55: 89-96.
131. 131. Cazzola M, Messinger D, Battistel V, et al. Recombinant human erythropoietin in the anemia associated with multiple myeloma or non-Hodgkin lymphoma:dose finding and identification of predictors of response. Blood 1995; 86: 4446-4453.
132. 132. Skikne BS, Cook JD. Effect of enhanced erythropoiesis on iron absorption. J Lab Clin Med 1992; 120: 746-751.
133. 133. Major A, Bauer C, Breymann C, et al. rh-erythropoietin stimulates immature reticulocyte release in man. Br J Haematol 1994; 87: 605-608.
134. 134. Cazzola M, Ponchio L, Pedrotti C, et al. Prediction of response to recombinant human erythropoietin (rHuEpo) in anemia of malignancy. Haematologica 1996; 81: 434-441.
135. 135. Looker AC, Dallman PR, Carroll M D, et al. Prevalence of iron deficiency in the United States. JAMA 1997; 277: 973-976.
136. 136. Lozoff B, Jimenez E, Wolf A. Long-term developmental outcome of infants with iron deficiency. N Engl J Med 1991; 325: 687-694.
137. 137. Oski FA. Iron deficiency in infancy and childhood. N Engl J Med 1993; 329: 190-193.
138. 138. Bruner AB, Joffe A, Duggan A K, et al. Randomised study of cognitive effects of iron supplementation in non-anaemic iron-deficienct adolescent girls. Lancet 1996; 348: 992-996.
139. 139. Brugnara C, Zurakowski D, DiCanzio J, et al. Reticulocyte hemoglobin content to diagnose iron deficiency in children. JAMA 1998; 281: 2225-2230.
140. 140. Root CB. CodeMap™ 98 for Laboratory and Pathology Services. . Barrington, IL: Ventura Resources, 1998.
141. 141. Lafferty JD, Crowther MA, Ali MA, et al. The evaluation of various mathematical RBC indices and their efficacy in discriminating between thalassemic and non-thalassemic microcytosis. Am J Clin Pathol 1996; 106: 201-205.
142. 142. d'Onofrio G, Zini G, Ricerca BM, et al. Automated measurement of red blood cell microcytosis and hypochromia in iron deficiency and ß-thalassemia trait. Arch Pathol Lab Med 1992; 116: 84-89.
143. 143. Chirillo R, Caenaro GF. The evaluation of erythrocyte regeneration by the H*3 hematology analyzer. In: F. Balzac, S. Chapman and C. Verderese, eds. H*3: new perspectives for hematology. Pp. 40-41. Tarrytown, NY, Bayer Diagnostics, 1993.
144. 144. Brugnara C. Use of reticulocyte cellular indices in the diagnosis and treatment of hematological disorders. Int J Clin Lab Res 1998; 28: 1-11.
145. 145. Patton WN, Cave RJ Harris RI. A study of changes in red cell volume and haemoglobin concentration during phlebotomy-induced iron deficiency and iron repletion using the Technicon H1. Clin Lab Haematol 1991; 13: 153-161.
146. 146. Bessman D. Erythropoiesis during recovery from macrocytic anemia: macrocytes, normocytes and microcytes. Blood 1977; 50: 995-1000.
147. 147. Mohandas N. Reticulocyte biology in red blood cell disorders. In: F. Balzac, S. Chapman and C. Verderese, eds. H*3: New Perspectives for Hematology. Pp. 46-47. Tarrytown, NY, Bayer Diagnostics, 1993.
148. 148. De Franceschi L, Bachir D, Galacteros F, et al. Oral magnesium supplements reduce erythrocyte dehydration in patients with sickle cell disease. J Clin Invest 1997; 100: 1847-1852.
149. 149. De Franceschi L, Bachir D, Galacteros F, et al. Dietary magnesium supplementation reduces pain crises in patients with sickle cell disease. Blood 1997; 90: 264a (abstr.).
150. 150. De Franceschi L, Cappellini MD, Graziadei G, et al. The effect of dietary magnesium supplementation on the cellular abnormalities of erythrocytes in patients with beta thalassemia intermedia. Haematologica 1998; 83: 118-25.
151. 151. Lazarus HM, Chahine A, Lacerna K, et al. Kinetics of erythrogenesis after bone marrow transplantation. Am J Clin Pathol 1992; 97: 574-583.
152. 152. Davies S, Cavill I, Bentley N, et al. Evaluation of erythropoiesis after bone marrow transplantation: quantitative reticulocyte counting. Br J Haematol 1992; 81: 12-17.
153. 153. Kanold J, Bezou M, Coulet M, et al. Evaluation of erythropoietic/hematopoietic reconstitution after BMT by highly fluorescent reticulocyte counts compares favorably with traditional peripheral blood cell counting. Bone Marrow Transplant 1993; 11: 313-318.
154. 154. Greinix HT, Linkesch W, Keil F, et al. Early detection of hematopoietic engraftment after bone marrow and peripheral blood stem cell transplantation by highly fluorescent reticulocyte counts. Bone Marrow Transplant 1994; 14: 307-313.
155. 155. Batjer JD, Riddell K Fritsma GA. Predicting bone marrow transplant engraftment by automated flow cytometric reticulocyte analysis. Lab Med 1994; 25: 22-26.
156. 156. Kabata J, Tichelli A, Gratwohl A , et al. Flow cytometric pattern of leucocyte recovery after therapy-induced aplasia. Acta Haematol Polon 1994; 25: 329-342.
157. 157. Remacha A. Flow cytometric reticulocyte quantification in the evaluation of hematologic recovery. Eur J Haematol 1994; 53: 293-297.
158. 158. Santamaria A 130, Martino R, Bellido M, et al. Reticulocyte recovery is faster in allogeneic and autologous peripheral blood stem cell transplantation than in bone marrow transplantation. Eur J Haematol 1997; 58: 362-364.
159. 159. Sica S, Salutari P, Laurenti L, et al. Highly fluorescent reticulocytes after CD34+ peripheral blood progenitor cell transplantation. Bone Marrow Transplant 1998; 21: 361-4.
160. 160. Testa U, Rutella S, Martucci R, et al. Autologous stem cell transplantation: evaluation of erythropoietic reconstitution by highly fluorescent reticulocyte counts, erythropoietin, soluble transferrin receptors, ferritin, TIBC and iron dosages. Br J Haematol 1997; 96: 762-75.
161. 161. Remacha AF, Martino R, Sureda A et al. Changes in reticulocyte fractions during peripheral stem cell harvesting: role in monitoring stem cell collection. Bone Marrow Transplant 1996; 17: 163-168.
162. 162. Biesma DH, Kraaijenhagen RJ, Dalmulder J, et al. Recombinant human erythropoietin in autologous blood donors: a dose-finding study. Br J Haematol 1994; 86: 30-35.
163. 163. Musto P, Falcone A, D'Arena G, et al. Clinical results of recombinant erythropoietin in transfusion-dependent patients with refractory multiple myeloma: role of cytokines and monitoring of erythropoiesis. Eur J Haematol 1997; 58: 314-9.
164. 164. Tsuda I, Tatsumi N. Maturity of reticulocytes in various hematological disorders. Eur J Haematol 1989; 43: 252-254.
165. 165. Chang CC, Kass L. Clinical significance of immature reticulocyte fraction determined by automated reticulocyte counting. Am J Clin Pathol 1997; 108: 69-73.
166. 166. Böck A, Herkner KR. Reticulocyte maturity pattern analysis as a predictive marker of erythropoiesis in paediatrics. Part II. Pilot study for clinical application. Clin Lab Haematol 1994; 16: 343-348.
167. 167. Khuhapinant A, Bunyaratvej A, Tatsumi N, et al. Number and maturation of reticulocytes in various genotypes of thalassaemia as assessed by flow cytometry. Acta Haematol 1994; 91: 119-25.
168. 168. Brugnara C, Platt O. The neonatal erythrocyte and its disorders. In: Nathan DG and Orkin SH, eds. Hematology of infancy and childhood. pp. 19-52. Philadelphia, W.B. Saunders, 1998.