Expression and use of the green fluorescent protein as a reporter system in Legionella pneumophila

Molecular and General Genetics

Volumn 262, Issue 6, 2000, Pages 1060-1069

  Kohler R ^a   Bubert, A ^a   Goebel, W ^a   Steinert, M ^a   Hacker, J ^a   Bubert, B ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Acanthamoeba castellanii; Green fluorescent protein (GFP); Intracellular survival; Legionella pneumophila; Macrophage infectivity potentiator (Mip) protein

Indexed keywords

CYCLOHEXIMIDE; CYTOCHALASIN D; GREEN FLUORESCENT PROTEIN; METHYLAMINE; SUPEROXIDE DISMUTASE;

ACANTHAMOEBA CASTELLANII; ARTICLE; BACTERIAL INFECTION; BACTERIAL VIRULENCE; CELL DIVISION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; GENE FUSION; GENETIC TRANSFORMATION; LEGIONELLA PNEUMOPHILA; LISTERIA MONOCYTOGENES; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE;

ACANTHAMOEBA CASTELLANII; BACTERIA (MICROORGANISMS); LEGIONELLA PNEUMOPHILA; LISTERIA MONOCYTOGENES; NEGIBACTERIA;

EID: 0033979096     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/PL00008649     Document Type: Article

References (56)

1. Abu Kwaik Y (1996) The phagosome containing Legionella pneumophila within the protozoan Hartmanella vermiformis is surrounded by the rough endoplasmic reticulum. Appl Environ Microbiol 62:2022-2028
2. Abu Kwaik Y (1998) Induced expression of the Legionella pneumophila gene encoding a 20-kilodalton protein during intracellular infection. Infect Immun 66:203-212
3. Abu Kwaik Y, Eisenstein, BI, Engleberg NC (1993) Phenotypic modulation by Legionella pneumophila upon infection of macrophages. Infect Immun 61:1320-1329
4. Abu Kwaik Y, Fields B, Engleberg NC (1994) Protein expression by the protozoan Hartmannella vermiformis upon contact with its bacterial parasite Legionella pneumophila. Infect Immun 62:1860-1866
5. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S (1998) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 64:2240-2246
6. Barker J, Lambert PA, Brown MRW (1993) Influence of intraamoebic and other growth conditions on the surface properties of Legionella pneumophila. Infect Immun 61:3503-3510
7. Berger KH, Merriam JJ, Isberg RR (1994) Altered intracellular targeting properties associated with mutations in the Legionella dotA gene. Mol Microbiol 14:809-822
8. Bubert A, Sokolovic Z, Chun S-K, Papatheodorou L, Simm A, Goebel W (1999) Differential expression of Listeria monocytogenes virulence genes in mammalian host cells. Mol Gen Genet 261:323-336
9. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263:802-805
10. Cianciotto NP, Fields B (1992) Legionella pneumophila mip gene potentiates intracellular infection of protozoa and human macrophages. Proc Natl Acad Sci USA 89:5188-5191
11. Cirillo JD, Tompkins LS, Falkow S (1994) Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion. Infect Immun 62:3254-3261
12. Cormack BP, Valdivia R, Falkow S (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38
13. Dehio M, Knorre A, Lanz C, Dehio C (1998) Construction of versatile high-level expression vectors for Bartonella henselae and the use of green fluorescent protein as a new expression marker. Gene 30:223-229
14. Dhandayuthapani S, Via LE, Thomas CA, Horowitz PM, Deretic D, Deretic V (1995) Green fluorescent protein as a marker for gene expression and cell biology of mycobacterial interactions with macrophages. Mol Microbiol 17:901-912
15. Doyle RM, Steele TW, McLennan AM, Parkinson IH, Manning PA, Heuzenroeder MW (1998) Sequence analysis of the mip gene of the soilborne pathogen Legionella pneumophila. Infect Immun 66:1492-1499
16. Engleberg NC, Carter C, Weber DR, Cianciotto NP, Eisenstein BI (1989) DNA sequence of mip, a Legionella pneumophila gene associated with macrophage infectivity. Infect Immun 57:1263-1270
17. Feeley JC, Gibson RJ, Gorman GW, Lanfford NC, Rasheed JK, Mackel DC, Baine WB (1979) Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol 10:437-441
18. Fields BS (1996) The molecular ecology of legionellae. Trends Microbiol 4:286-290
19. Gao L-Y, Harb OS, Abu Kwaik Y (1997) Utilization of similar mechanisms by Legionella pneumophila to parasitize two evolutionarily distant hosts, mammalian and protozoan cells. Infect Immun 65:4738-4746
20. Gao L-Y, Harb OS, Abu Kwaik Y (1998) Identification of macrophage-specific infectivity loci (mil) of Legionella pneumophila that are not required for infectivity of protozoa. Infect Immun 66:883-892
21. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580
22. Harb OS, Venkataraman C, Haack BJ, Gao L-Y, Abu Kwaik Y (1998) Heterogeneity in the attachment and uptake mechanisms of the Legionnaires' disease bacterium, Legionella pneumophila, by protozoan hosts. Appl Environ Microbiol 64:126-132
23. Heim R, Prasher DC, Tsien RY (1994) Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA 91:12501-12504
24. Heuner K, Hacker J, Brand BC (1997) The alternative sigma factor sigma 28 of Legionella pneumophila restores flagellation and motility to an Escherichia coli fliA mutant. J Bacteriol 179:17-23
25. Horwitz MA (1983a) Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes. J Exp Med 158:1319-1331
26. Horwitz MA (1983b) The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes. J Exp Med 158:2108-2126
27. Horwitz MA (1984) Phagocytosis of the Legionnaires' disease bacterium (Legionella pneumophila) occurs by a novel mechanism: engulfment within a pseudopod coil. Cell 36:27-33
28. Horwitz MA (1987) Characterization of avirulent mutant Legionella pneumophila that survive but do not multiply within human monocytes. J Exp Med 166:1310-1328
29. Horwitz MA, Maxfield FR (1984) Legionella pneumophila inhibits acidification of its phagosome in human monocytes. J Cell Biol 99:105-114
30. Jepras RI, Fitzgeorge RB, Baskerville A (1985) A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea-pigs. J Hyg (Lond) 95:29-38
31. Josenhans C, Friedrich S, Suerbaum S (1998) Green fluorescent protein as a novel marker and reporter system in Helicobacter sp. FEMS Microbiol Lett 15:263-273
32. King CH, Fields BS Shotts EB, White EH (1991) Effects of cytochalasin D and methylamine on intracellular growth of Legionella pneumophila in amoebae and human monocyte-like cells. Infect Immun 59:758-763
33. Kremer L, Baulard A, Estaquier J, Poulain-Godefroy O, Locht C (1995) Green fluorescent protein as a new expression marker in mycobacteria. Mol Microbiol 17:913-922
34. Lee AK, Falkow S (1998) Constitutive and inducible green fluorescent protein expression in Bartonella henselae. Infect Immun 66:3964-3967
35. Ludwig B, Rahfeld J, Schmidt B, Mann K, Wintermeyer E, Fischer G, Hacker J (1994) Characterization of Mip proteins of Legionella pneumophila. FEMS Microbiol Lett 118:23-30
36. Marra A, Blander SJ, Horwitz MA, Shuman HA (1992) Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc Natl Acad Sci USA 89:9607-9611
37. McClain MS, Hurley MC, Brieland JK, Engleberg NC (1996) The Legionella pneumophila hel locus encodes intracellularly induced homologs of heavy-metal ion transporters of Alcaligenes spp. Infect Immun 64:1532-1540
38. Payne NR, Horwitz MA (1987) Phagocytosis of Legionella pneumophila is mediated by human monocyte complement receptors. J Exp Med 166:1377-1389
39. Purcell M, Shuman HA (1998) The Legionella pneumophila icmGCDJBF genes are required for killing of human macrophages. Infect Immun 66:2245-2255
40. Pruckler JM, Benson RF, Moyenuddin M, Martin WT, Fields BS (1995) Association of flagellum expression and intracellular growth of Legionella pneumophila. Infect Immun 63:4928-4932
41. Raffelsbauer D, Bubert A, Engelbrecht F, Scheinpflug J, Simm A, Hess J, Kaufmann SH, Goebel W (1998) The gene cluster inlC2DE of Listeria monocytogenes contains additional new internalin genes and is important for virulence in mice. Mol Gen Genet 260:144-58
42. Ratcliff RM, Donnellan SC, Lanser JA, Manning PA, Heuzenroeder MW (1997) Interspecies sequence differences in the Mip protein from the genus Legionella: implications for function and evolutionary relatedness. Mol Microbiol 25:1149-1158
43. Rechnitzer C, Blom J (1989) Engulfment of the Philadelphia strain of Legionella pneumophila within pseudopod coils in human phagocytes. APMIS 97:105-114
44. Rowbotham TJ (1986) Current views on the relationships between amoebae, legionellae and man. Isr J Med Sci 22:678-689
45. Sadosky AB, Wiater LA, Shuman HA (1993) Identification of Legionella pneumophila genes required for growth within and killing of human macrophages. Infect Immun 61:5361-5373
46. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
47. Segal G, Shuman HA (1997) Characterization of a new region required for macrophage killing by Legionella pneumophila. Infect Immun 65:5057-5066
48. Steinert M, Ott M, Lück PC, Hacker J (1994) Studies on the uptake and intracellular replication of Legionella pneumophila in protozoa and in macrophage-like cells. FEMS Microbiol Ecol 15:299-308
49. Stone BJ, Abu Kwaik Y (1998) Expression of multiple pili by Legionella pneumophila: identification and characterization of a type IV pilin gene and its role in adherence to mammalian and protozoan cells. Infect Immun 66:1768-1775
50. Susa M, Hacker J, Marre R (1996) De novo synthesis of Legionella pneumophila antigens during intracellular growth in phagocytic cells. Infect Immun 64:1679-1684
51. Tombolini R, Unge A, Davey ME, de Bruijn FJ, Jansson JK (1997) Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria. FEMS Microbiol Ecol 22:17-28
52. Valdivia RH, Falkow S (1996) Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol Microbiol 22:367-378
53. Valdivia RH, Hromockyj AE, Monack D, Ramakrishnan L, Falkow S (1996) Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene 173:47-52
54. Webb CD, Decatur A, Teleman A, Losick R (1995) Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis. J Bacteriol 177:5906-5911
55. Wintermeyer E, Ludwig B, Steinert M, Schmidt B, Fischer G, Hacker J (1995) Influence of site specifically altered Mip proteins on intracellular survival of Legionella pneumophila in eukaryotic cells. Infect Immun 63:4576-4583
56. Zhao H, Thompson RB, Lockatell V, Johnson DE, Mobley HL (1998) Use of green fluorescent protein to assess urease gene expression by uropathogenic Proteus mirabilis during experimental ascending urinary tract infection. Infect Immun 66:330-335