Evidence for a novel affinity mechanism of motor-assisted transport along microtubules

Molecular Biology of the Cell

Volumn 11, Issue 1, 2000, Pages 161-169

  Wada, Yuuko ^a   Hamasaki, Toshikazu ^a   Satir, Peter ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA TUBULIN; DYNEIN ADENOSINE TRIPHOSPHATASE; KINESIN;

ARTICLE; CELL ADHESION; CELL FUNCTION; CELL MOTION; DYNAMICS; MEMBRANE TRANSPORT; MICROTUBULE; MOTOR ACTIVITY; PRIORITY JOURNAL;

EID: 0033978565     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.11.1.161     Document Type: Article

References (27)

1. Barkalow, K., Avolio, J., Holwill, M.E.J., Hamasaki, T., and Satir, P. (1994). Structural and geometrical constraints on the outer dynein arm in situ. Cell Motil. Cytoskeleton 27, 299-312.
2. Block, S.M., Goldstein, L.S.B., and Schnapp, B.J. (1990). Bead movement by single kinesin molecules. Studied with optical tweezers. Nature 348, 348-352.
3. Fan, J., Griffiths, A.D., Lockhar, A., Cross, R.A., and Amos, L.A. (1996). Microtubule minus ends can be labeled with a phage display antibody specific to alpha-tubulin. J. Mol. Biol. 259, 325-330.
4. Frens, G. (1973). Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature 241, 20-22.
5. Gill, S.R., Schroer, T.A., Szilak, I., Steuer, E.R., Sheetz, M.P., and Cleveland, D.W. (1991). Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein. J. Cell Biol. 175, 1639-1650.
6. Gozes, I., and Barnstable, C.J. (1982). Monoclonal antibodies that recognize discrete forms of tubulin. Proc. Natl. Acad. Sci. USA 79, 2579-2583.
7. Grancell, A., and Sorger, P.K. (1998). Chromosome movement: kinetochores motor along. Curr. Biol. 8, R382-R385.
8. Gundersen, G.G., and Cook, T.A. (1999). Microtubules and signal transduction. Curr. Opin. Cell Biol. 11, 81-94.
9. Hamasaki, T., Barkalow, K., Richmond, J., and Satir, P. (1991). A cAMP-stimulated phosphorylation of an axonemal polypeptide that copurifies with the 22S dynein arm regulates microtubule translocation velocity and swimming speed in Paramecium. Proc. Natl. Acad. Sci. USA 88, 7918-7922.
10. Hamasaki, T., Holwill, M.E.J., Barkalow, K., and Satir, P. (1995). Mechanochemical aspects of axonemal dynein activity studied by in vitro microtubule translocation. Biophys. J. 69, 2569-2579.
11. Hancock, W.O., and Howard, J. (1998). Processivity of the motor protein kinesin requires two heads. J. Cell Biol. 140, 1395-1405.
12. Hoenger, A., Sablin, E.P., Vale, R.D., Fletterick, R.J., and Milligan, R.A. (1995). Three-dimensional structure of a tubulin-motor-protein complex. Nature 376, 271-274.
13. Jiang, W., Stock, M.F., Li, X., and Hackney, D.D. (1997). Influence of the kinesin neck domain on dimerization and ATPase kinetics. J. Biol. Chem. 272, 7626-7632.
14. Koshland, D.E., Mitchison, T.J., and Kirschner, M.W. (1988). Polewards chromosome movement driven by microtubule depolymerization in vitro. Nature 331, 499-504.
15. LeDizet, M., and Piperno, G. (1987). Identification of an acetylation site of Chlamydomonas α-tubulin. Proc. Natl. Acad. Sci. USA 84, 5720-5724.
16. Nogales, E., Whittaker, M., Milligan, R.A., and Downing, K.H. (1999). High-resolution model of the microtubule. Cell 96, 79-88.
17. Nogales, E., Wolf, S.G., and Downing, K.H. (1998). Structure of the αβ tubulin dimer by electron crystallography. Nature 391, 199-203.
18. Okada, Y., and Hirokawa, N. (1999). A processive single-headed motor: kinesin superfamily protein KIF1A. Science 283, 1152-1157.
19. Rieder, C.L., and Salmon, E.D. (1998). The vertebrate cell kinetochore and its roles during mitosis. Trends Cell Biol. 8, 310-317.
20. Song, Y.H., and Mandelkow, E. (1995). The anatomy of flagellar MTs: polarity, seam, junctions, and lattice. J. Cell Biol. 128, 81-94.
21. Uyeda, T.Q.P., Kron, S.J., and Spudich, J.A. (1990). Myosin step size estimation from slow sliding movement of actin over low densities of heavy meromyosin. J. Mol. Biol. 214, 699-710.
22. Vale, R.D., Malik, F., and Brown, D. (1992). Directional instability of microtubule transport in the presence of kinesin and dynein, two opposite polarity motors. J. Cell Biol. 119, 1589-1596.
23. Vale, R.D., Soll, D.R., and Gibbons, I.R. (1989). One-dimensional diffusion of microtubules bound to flagellar dynein. Cell 59, 915-925.
24. Vale, R.D., and Toyoshima, Y.Y. (1989). Microtubule translocation properties of intact and proteolytically digested dyneins from Tetrahymena cilia. J. Cell Biol. 108, 2327-2334.
25. Vaughan, K.T., and Vallee, R.B. (1995). Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued. J. Cell Biol. 131, 1507-1516.
26. Wade, R.H., and Hyman, A.A. (1997). Microtubule structure and dynamics. Curr. Opin. Cell Biol. 9, 12-17.
27. Williams, R.C., and Lee, J.C. (1982). Preparation of tubulin from brain. Methods Enzymol. 85, 376-381.