Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger

Microbiology

Volumn 146, Issue 2, 2000, Pages 415-426

  Gordon, Caroline L ^a   Khalaj, Vahid ^a   Ram, Arthur F J ^b   Archer, David B ^c   Brookman, Jayne L ^a   Trinci, Anthony P J ^a   Jeenes, David J ^c   Doonan, John H ^e   Wells, Brian ^e   Punt, Peter J ^d   Van Den Hondel, Cees A M J J ^d   Robson, Geoffrey D ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b LEIDEN UNIVERSITY   (Netherlands)

^c INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^d TNO   (Netherlands)

^e JOHN INNES CENTRE   (United Kingdom)

Author keywords

Aspergillus niger; Glucoamylase; Green fluorescent protein (GFP); Heterologous protein production; Protein secretion

Indexed keywords

GLUCAN 1,4 ALPHA GLUCOSIDASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARTICLE; ASPERGILLUS NIGER; CELL WALL; FLUORESCENCE; FLUORESCENCE MICROSCOPY; FUNGUS CULTURE; FUNGUS MUTANT; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION; WESTERN BLOTTING;

EID: 0033965771     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-146-2-415     Document Type: Article

References (50)

1. Archer, D. B. & Peberdy, J. F. (1997). The molecular biology of secreted enzyme production by fungi. Crit Rev Biotechnol 17, 273-306.
2. Archer, D. B., Roberts, I. N. & MacKenzie, D. A. (1990). Heterologous protein secretion from Aspergillus niger in phosphate-buffered batch culture. Appl Microbiol Biotechnol 34, 313-315.
3. Archer, D. B., Jeenes, D. J. & MacKenzie, D. A. (1994). Strategies for improving heterologous protein production from filamentous fungi. Antonie Leeuwenhoek 65, 245-250.
4. Braun, E. L. Kang, S. C., Nelson, M. A. & Natvig, D. O. (1998). Identification of the first fungal annexin: analysis of annexin gene duplications and implications for eukaryotic evolution. J Mol Evol 47, 531-543.
5. Broekhuijsen, M. P., Mattern, I. E., Contreras, R., Kinghorn, J. R. & van den Hondel, C. A. M. J. J. (1993). Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein. J Biotechnol 31, 135-145.
6. Chalfie, M. (1995). Green fluorescent protein. Photochem Photobiol 62, 651-656.
7. Chiu, W.-L., Niwa, Y., Zeng, W., Hirano, T., Kobayashi, H. & Sheen, J. (1996). Engineered GFP as a vital reporter in plants. Curr Biol 6, 325-330.
8. Contreras, R., Carrez, D., Kinghorn, J. R., van den Hondel, C. A. M. J. J. & Fiers, W. (1991). Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6. Biotechnology 9, 378-381.
9. Cormack, B. P., Bertram, G., Egerton, M., Gow, N. A. R., Falkow, S. & Brown, A. J. P. (1997). Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans. Microbiology 143, 303-311.
10. Durand, H., Clanet, M. & Tiraby, G. (1988). Genetic improvement of Trichoderma reesei for large scale cellulase production. Enzyme Microb Technol 10, 341-345.
11. Fernández-Ábalos, J. M., Fox, H., Pitt, C., Wells, B. & Doonan, J. H. (1998). Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus Aspergillus nidulans. Mol Microbiol 27, 121-130.
12. Finkelstein, D. B. (1987). Improvement of enzyme production in Aspergillus. Antonie Leeuwenhoek 53, 349-352.
13. Fowler, T., Berka, R. M. & Ward, M. (1990). Regulation of the glaA gene in Aspergillus niger. Curr Genet 18, 537-545.
14. Gouka, R. J., Punt, P. J., Hessing, J. G. M. & van den Hondel, C. A. M. J. J. (1996). Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains. Appl Environ Microbiol 62, 1951-1957.
15. Gouka, R. J., Punt, P. J. & van den Hondel, C. A. M. J. J. (1997a). Efficient production of secreted proteins by Aspergillus : progress, limitations and prospects. Appl Microbiol Biotechnol 47, 1-11.
16. Gouka, R. J., Punt, P. J. & van den Hondel, C. A. M. J. J. (1997b). Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels. Appl Environ Microbiol 63, 488-497.
17. Haas, J., Park, E.-C. & Seed, B. (1996). Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol 6, 315-324.
18. Harris, S. D., Morrell, J. L. & Hamer, J. E. (1994). Identification and characterization of Aspergillus nidulans mutants defective in cytokinesis. Genetics 136, 517-532.
19. van Hartingsveldt, W., Mattern, I. E., van Zeijl, C. M. J., Pouwels, P. H. & van den Hondel, C. A. M. J. J. (1987). Development of a homologous transformation system for Aspergillus niger based on the pyrG gene. Mol Gen Genet 206, 71-75.
20. van den Homberg, J. P. T. W., van de Vondervoort, P. J. I., Fraissinet-Tachet, L. & Visser, J. (1997). Aspergillus as a host for heterologous protein production, the problem of proteases. Trends Biotechnol 15, 256-263.
21. Heim, R., Cubitt, A. B. & Tsien, R. Y. (1995). Improved green fluorescence. Nature 373, 663-664.
22. Jeenes, D. J., MacKenzie, D. A. & Archer, D. B. (1994). Transcriptional and post-translational events affect the production of secreted hen egg white lysozyme by Aspergillus niger. Transgenic Res 3, 297-303.
23. Kneen, M., Farinas, J., Li, Y. & Verkman, A. S. (1998). Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys J 74, 1591-1599.
24. MacKenzie, D. A., Gendron, L. C. G., Jeenes, D. J. & Archer, D. B. (1994). Physiological optimization of secreted protein production by Aspergillus niger. Enzyme Microb Technol 16, 276-280.
25. Mattern, I. E., van Noort, J. M., van den Berg, P., Roberts, I. N., Archer, D. B. & van den Hondel, C. A. M. J. J. (1992). Isolation and characterization of mutants of Aspergillus niger deficient in extacellular proteases. Mol Gen Genet 234, 332-336.
26. Moukha, S. M., Wösten, H. A. B., Asther, M. & Wessels, J. G. H. (1993). In situ localization of the secretion of lignin peroxidases in colonies of Phanerochaete chrysosporium using a sandwiched mode of culture. J Gen Microbiol 139, 969-978.
27. de Nobel, J. G. & Barnett, J. A. (1991). Passage of molecules through yeast cell walls - a brief essay review. Yeast 7, 313-323.
28. Nyyssönen, E. & Keränen, S. (1995). Multiple roles of the cellulase CBHI in enhancing production of fusion antibodies by the filamentous fungus Trichoderma reesei. Curr Genet 28, 71-79.
29. Patterson, G. H., Knobel, S. M., Sharif, W. D., Kain, S. R. & Piston, D. W. (1997). Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys J 73, 2782-2790.
30. Punt, P. J., Oliver, R. P., Dingemanse, M. A., Pouwels, P. H. & van den Hondel, C. A. M. J. J. (1987). Transformation of filamentous fungi based on the hygromycin B resistance marker from Escherichia coli. Gene 56, 117-124.
31. Punt, P. J. & van den Hondel, C. A. M. J. J. (1992). Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. Methods Enzymol 216, 447-457.
32. Roberts, I. N., Jeenes, D. J., MacKenzie, D. A., Wilkinson, A. P., Sumner, I. G. & Archer, D. B. (1992). Heterologous gene expression in Aspergillus niger, a glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme. Gene 122, 155-161.
33. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
34. Schrickx, J. M., Krave, A. S., Verdoes, J. C. & van den Hondel, C. A. M. J. J. (1993). Growth and product formation in chemostat and recycling cultures by Aspergillus niger N402 and a glucoamylase overproducing transformant, provided by multiple copies of the glaA gene. J Gen Microbiol 139, 2801-2810.
35. Sheen, J., Hwang, S., Niwa, Y., Kobayashi, H. & Galbraith, D. W. (1995). Green-fluorescent protein as a new vital marker in plant cells. Plant J 8, 777-784.
36. Siedenberg, D., Mestric, S., Ganzlin, M., Schmidt, M., Punt, P. J., van den Hondel, C. A. M. J. J. & Rinas, U. (1999). GlaA promoter controlled production of a mutant green fluorescent protein (S65T) by recombinant Aspergillus niger during growth on defined medium in hatch and fed-batch cultures. Biotechnol Prog 15, 43-50.
37. Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98, 503-517.
38. Spellig, T., Bottin, A. & Kahmann, R. (1996). Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Mol Gen Genet 252, 503-509.
39. Suelmann, R., Sievers, N. & Fischer, R. (1997). Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans. Mol Microbiol 25, 757-769.
40. Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proc Natl Acad Sci USA 76, 4350-4354.
41. Trevithick, J. R. & Metezenberg, R. L. (1966). Molecular sieving by Neurospora crassa cell walls during secretion of invertase isoenzymes. J Bacteriol 92, 1010-1015.
42. Veldhuisen, G., Saloheimo, M., Fiers, M. A., Punt, P. J., Contreras, R., Penttila, M. & van den Hondel, C. A. M. J. J. (1997). Isolation and analysis of functional homologues of the secretion-related SAR1 gene of Saccharomyces cerevisiae from Aspergillus niger and Trichoderma reesei. Mol Gen Genet 256, 446-455.
43. Verdoes, J. C., Punt, P. J., Strouthamer, A. H. & van den Hondel, C. A. M. J. J. (1994). The effect of multiple copies of the upstream region on expression of the Aspergillus niger glucoamylase-encoding gene. Gene 145, 179-187.
44. Vogel, H. J. (1956). A convenient growth medium for Neurospora (medium N). Microb Genet Bull 13, 42-44.
45. Ward, M., Wilson, L. J., Kodama, K. H., Rey, M. W. & Berka, R. M. (1990). Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion. Biotechnology 8, 435-440.
46. Wessels, J. G. H. (1994). Developmental regulation of fungal cell wall formation. Annu Rev Phytopathol 32, 413-437.
47. Witteveen, C. F. B., Veenhuis, M. & Visser, J. (1992). Localisation of glucose oxidase and catalase activities in Aspergillus niger. Appl Environ Microbiol 58, 1190-1194.
48. Wösten, H. A. B., Moukha, S. M., Sietsma, J. H. & Wessels, J. G. H. (1991). Localization of growth and secretion of proteins in Aspergillus niger. J Gen Microbiol 137, 2017-2023.
49. van den Wymelenberg, A. J., Cullen, D., Spear, R. N., Schoenike, B. & Andrews, J. H. (1997). Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces. Biotechniques 23, 686-690.
50. van Zeijl, C. M. J., van de Kamp, E. H. M., Punt, P. J., Selten, G. C. M., Hauer, B., van Gorcom, R. F. M. & van den Hondel, C. A. M. J. J. (1998). An improved colony-PCR method for filamentous fungi for amplication of PCR fragments of several kilobases. J Biotechnol 59, 221-224.