Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post)translational levels

Applied and Environmental Microbiology

Volumn 63, Issue 2, 1997, Pages 488-497

  Gouka, Robin J ^a,b   Punt, Peter J ^a   Van Den Hondel, Cees A M J J ^a  

^a TNO   (Netherlands)

^b UNILEVER RESEARCH VLAARDINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA GALACTOSIDASE; FUNGAL PROTEIN; GLUCAN 1,4 ALPHA GLUCOSIDASE; INTERLEUKIN 6; MESSENGER RNA; XYLAN 1,4 BETA XYLOSIDASE;

ARTICLE; ASPERGILLUS AWAMORI; ASPERGILLUS NIGER; CONTROLLED STUDY; EXPRESSION VECTOR; FUNGUS GROWTH; GENE FUSION; GENETIC TRANSCRIPTION; NONHUMAN; PROMOTER REGION; PROTEIN PROCESSING;

ASPERGILLUS AWAMORI; ASPERGILLUS NIGER; CYAMOPSIS TETRAGONOLOBA; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0030942270     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.2.488-497.1997     Document Type: Article

References (43)

1. Bergkamp, R. J. M., I. M. Kool, R. H. Geerse, and R. J. Planta. 1992. Multiple-copy integration of the α-galactosidase gene from Cyamopsis tetragonoloba into the ribosomal DNA of Kluyveromyces lactis. Curr. Genet. 21:365-370.
2. Berka, R. M., F. T. Bayliss, P. Bloebaum, D. Cullen, N. S. Dunn-Coleman, K. H. Kodama, K. J. Hayenga, R. A. Hitzeman, M. A. Lamsa, M. M. Przetak, M. W. Rev, L. J. Wilson, and M. Ward. 1991. Aspergillus niger var. awamori as a host for the expression of heterologous genes, p. 273-292. In J. W. Kelly and T. O. Baldwin (ed.), Applications of enzyme biotechnology. Plenum Press, New York, N.Y.
3. Broekhuijsen, M. P., I. E. Mattern, R. Contreras, J. R. Kinghorn, and C. A. M. J. J. van den Hondel. 1993. Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein. J. Biotechnol. 31:135-145.
4. Bruchez, J. J. P., J. Eberle, and V. E. A. Russo. 1993. Regulatory sequences in the transcription of Neurospora crassa genes: CAAT box, TATA box. introns, poly(A) tail formation sequences. Fungal Genet. Newsl. 40:89-96.
5. Carrez, D., W. Janssens, P. Degrave, C. A. M. J. J. van den Hondel, J. R. Kinghorn, W. Fiers, and R. Contreras. 1990. Heterologous gene expression by filamentous fungi: secretion of human interleukin-6 by Aspergillus nidulans. Gene 94:147-154.
6. Contreras, R., D. Carrez, J. R. Kinghorn, C. A. M. J. J. van den Hondel, and W. Fiers. 1991. Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by A. nidulans and secretion of mature interleukin-6. Bio/Technology 9:378-380.
7. Fellinger, A. J., J. M. A. Verbakel, R. A. Veale, P. E. Sudbery, I. J. Bom, N. Overbeeke, and C. T. Verrips. 1991. Expression of the α-galactosidase from Cyamopsis tetragonoloba (Guar) by Hansenula polymorpha. Yeast 7:463-473.
8. Finkelstein, D. B., J. Rambosek, M. S. Crawford, C. L. Soliday, P. C. McAda, and J. Leach. 1989. Protein secretion in Aspergillus niger, p. 295-300. In C. L. Hershberger, S. W. Queener, and G. Hegeman (ed.), Genetics and molecular biology of industrial microorganisms. American Society for Microbiology, Washington, D.C.
9. Fowler, T., and R. M. Berka. 1991. Gene expression systems for filamentous fungi. Curr. Opin. Biotechnol. 2:691-697.
10. Gouka, R. J., J. G. M. Hessing, H. Stam, W. Musters, and C. A. M. J. J. van den Hondel. 1995. A novel strategy for the isolation of defined pyrG mutants and the development of a site-specific integration system for Aspergillus awamori. Curr. Genet. 27:536-540.
11. Gouka, R. J., J. G. M. Hessing, P. J. Punt, H. Stam, W. Musters, and C. A. M. J. J. van den Hondel. 1996. An expression system based on the promoter region of the Aspergillus awamori 1,4-"-endoxylanase A gene. Appl. Microbiol. Biotechnol. 46:28-35.
12. Gouka, R. J., P. J. Punt, J. G. M. Hessing, and C. A. M. J. J. Van den Hondel. 1996. Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains. Appl. Environ. Microbiol. 62:1951-1957.
13. Guo, Z., and F. Sherman. 1995. 3′-end-forming signals of yeast mRNA. Mol. Cell. Biol. 15:5983-5990.
14. Gurr, S. J., S. E. Unkles, and J. R. Kinghorn. 1988. The structure and organization of nuclear genes of filamentous fungi, p. 93-139. In J. R. Kinghorn (ed.), Gene structure in eukaryotic microbes. IRL Press, Oxford, United Kingdom.
15. Hessing, J. G. M., C. van Rotterdam, J. M. A. Verbakel, M. Roza, J. Maat, R. F. M. van Gorcom, and C. A. M. J. J. van den Hondel. 1994. Isolation and characterization of a 1,4-beta-endoxylanase gene of A. awamori. Curr. Genet. 26:228-232.
16. Humphrey, T., and N. J. Proudfoot. 1988. A beginning to the biochemistry of polyadenylation. Trends Genet. 4:243-245.
17. Imiger, S., C. M. Egli, and G. H. Braus. 1991. Different classes of polyadenylation sites in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 11:3060-3069.
18. Jeenes, D. J., D. A. MacKenzie, and D. B. Archer. 1994. Transcriptional and post-transcriptional events affect the production of secreted hen egg white lysozyme by Aspergillus niger. Transgenic Res. 3:297-303.
19. Kinsey, J. A. 1989. A simple colony blot procedure for Neurospora. Fungal Genet. Newsl. 36:45-46.
20. Kolar, M., P. J. Punt, C. A. M. J. J. van den Hondel, and H. Schwab. 1988. Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene. Gene 62:127-134.
21. Manley, J. L. 1988. Polyadenylation of mRNA precursors. Biochim. Biophys. Acta 950:1-12.
22. Mateo-Rosell, A. Unpublished data.
23. Nyyssönen, E., M. Penttilä, A. Harkki, A. Saloheimo, J. K. C. Knowles, and S. Keränen. 1943. Efficient production of antibody fragments by the filamentous fungus Trichoderma reesei. Bio/Technology 11:591-595.
24. Nyyssönen, E., and S. Keränen. 1995. Multiple roles of the cellulase CBHI in enhancing production of fusion antibodies by the filamentous fungus Trichoderma reesei. Curr. Genet. 28:71-79.
25. Overbeeke, N., A. J. Fellinger, M. Y. Toonea, D. van Wassenaar, and C. T. Verrips. 1989. Cloning and nucleotide sequence of the α-galactosidase cDNA from Cyamopsis tetragonoloba (guar). Plant Mol. Biol. 13:541-550.
26. Overbeeke, N., G. H. M. Termorshuizen, M. L. F. Giuseppin, D. R. Underwood, and C. T. Verrips. 1990. Secretion of the α-galactosidase from Cyamopsis tetragonoloba (guar) by Bacillus subtilis. Appl. Environ. Microbiol. 56:1429-1434.
27. Proudfoot, N. 1991. Poly(A) signals. Cell 64:671-674.
28. Punt, P. J. Unpublished results.
29. Roberts, I. N., R. P. Oliver, P. J. Punt, and C. A. M. J. J. van den Hondel. 1989. Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Curr. Genet. 15:177-180.
30. 2 fusion protein is secreted and processed to yield mature enzyme. Gene 122:155-161.
31. Romanos, M. A., A. J. Makoff, N. F. Fainweather, K. M. Beesley, D. E. Slater, F. B. Rayment, M. M. Payne, and J. J. Clare. 1991. Expression of tetanus toxin fragment C in yeast: gene synthesis is required to eliminate fortuitous polyadenylation sites in AT-rich DNA. Nucleic Acids Res. 19:1461-1467.
32. Romanos, M. A., C. A. Scorer, and J. J. Clare. 1992. Foreign gene expression in yeast: a review. Yeast 8:423-488.
33. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
34. Schuren, F. H. J., T. R. van der Lende, and J. G. H. Wessels. 1993. Fruiting genes of Schizophyllum commune arc transcriptionally regulated. Mycol. Res. 97:538-542.
35. Scorer, C. A., R. G. Buckholz, J. J. Clare, and M. A. Romanos. 1993. The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136:111-119.
36. Van den Hondel, C. A. M. J. J., P. J. Punt, and R. F. M. van Gorcom. 1991. Heterologous gene expression in filamentous fungi, p. 396-428. In J. W. Bennet and L. L. Lasure (ed.), More gene manipulations in fungi. Academic Press, San Diego, Calif.
37. Van Gorcom, R. F. M., P. J. Punt, and C. A. M. J. J. van den Hondel. 1994. Heterologous gene expression in Aspergillus, p. 241-250. In K. A. Powell (ed.), The genus Aspergillus. Plenum Press, New York, N.Y.
38. Van Hartingsveldt, W., C. M. J. van Zeijl, A. E. Veenstra, J. A. van den Berg, P. H. Pouwels, R. F. M. van Gorcom, and C. A. M. J. J. van den Hondel. 1990. Heterologous gene expression in Aspergillus: analysis of chymosin production in single-copy transformants of A. niger, p. 107-116. In H. Heslot, J. Bavies, J. Florent, L. Bobichon, G. Durand, and L. Penasse (ed.), Proceedings of the 6th International Symposium on Genetics of Industrial Microorganisms. Société Francaise de Microbiologie, Strassbourg, France.
39. Verbakel, J. M. A. 1991. Heterologous gene expression in the yeast Saccharomyces cerevisiae. Ph.D. thesis. State University of Utrecht, Utrecht, The Netherlands.
40. Verdoes, J. C., P. J. Punt, and C. A. M. J. J. van den Hondel. 1995. Molecular genetic strain improvement for the overproduction of fungal proteins by filamentous fungi. Appl. Microbiol. Biotechnol. 43:195-205.
41. Ward, M., L. J. Wilson, K. H. Kodama, M. W. Rey, and R. M. Berka. 1990. Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion. Bio/technology 8:435-440.
42. Ward, P. P., C. S. Piddington, G. A. Cunningham, X. Zhou, R. D. Wyatt, and O. M. Conneely. 1995. A system for production of commercial quantities of. human lactoferrin: a broad spectrum natural antibiotic. Bio/Technology 13: 498-503.
43. Wickens, M. 1990. How the messenger got its tail: addition ot poly(A) in the nucleus. Trends Biochem. Sci. 15:277-281.