An improved colony-PCR method for filamentous fungi for amplification of pcr-fragments of several kilobases

Journal of Biotechnology

Volumn 59, Issue 3, 1998, Pages 221-224

  Van Zeijl, C M J ^a   Van De Kamp, E H M ^a   Punt, P J ^a   Selten, G C M ^b   Hauer, B ^c   Van Gorcom, R F M ^a   Van Den Hondel, C A M J J ^a  

^a TNO   (Netherlands)

^b Gist Brocades NV   (Netherlands)

^c BASF SE   (Germany)

Author keywords

Colony PCR method; Filamentous fungi; Rapid screening

Indexed keywords

FUNGI;

COLONY-PCR METHOD; FILAMENTOUS FUNGI;

BIOTECHNOLOGY;

ARTICLE; ASPERGILLUS NIGER; BACTERIUM TRANSFORMATION; FUNGUS; GENE DISRUPTION; GENETIC TRANSFORMATION; MYCELIUM; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ASPERGILLUS NIGER; CYTOCHROME P-450 ENZYME SYSTEM; DNA MUTATIONAL ANALYSIS; DNA, FUNGAL; ENZYMES; GLUCAN 1,4-ALPHA-GLUCOSIDASE; PLASMIDS; TRANSFORMATION, GENETIC;

ASPERGILLUS NIGER; BACTERIA (MICROORGANISMS); BASSIANA; BEAUVERIA; CORDYCEPS BASSIANA; FUNGI;

EID: 0032477490     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00170-3     Document Type: Article

References (7)

1. Arganoza M.T., Akins R.A. A modified colony-filter-hybridization protocol for filamentous fungi. Trends Genet. 11:1995;381-382.
2. Aufauvre-Brown A., Tang C.M., Holden D.W. Detection of gene-disruption events in Aspergillus transformants by polymerase chain reaction direct from conidiospores. Curr. Genet. 24:1993;177-178.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K., 1994. Current protocols in molecular biology, John Wiley and Sons Inc., USA.
4. Bennett, J.W., Lasure, L.L., 1991. Growth media. In: Bennett J.W., Lasure L.L. (Eds.). More Gene Manipulations in Fungi, Academic Press, San Diego, pp. 441-458.
5. Kinsey J.A. A simple colony blot procedure. Fungal Genet. Newsletter. 36:1989;45-46.
6. Van den Brink J.M., van Zeijl C.M.J., Brons J.F., van den Hondel C.A.M.J.J., van Gorcom R.F.M. Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger. DNA Cell. Biol. 14:1995;719-729.
7. Van Gorcom R.F.M., Boschloo J.B., Kuijvenhoven A., Lange J., van Vark A.J., Bos C.J., van Balken J.A.M., Pouwels P.H., van den Hondel C.A.M.J.J. Isolation and molecular characterisation of the benzoate-p-hydroxylase gene (bphA) of Aspergillus niger: a member of a new gene family of the cytochrome P450 superfamily. Mol. Gen. Genet. 223:1990;192-197.