Analysis of rdxA and involvement of additional genes encoding NAD(P)H flavin oxidoreductase (FrxA) and ferredoxin-like protein (FdxB) in metronidazole resistance of Helicobacter pylori

Antimicrobial Agents and Chemotherapy

Volumn 44, Issue 8, 2000, Pages 2133-2142

  Kwon, Dong Hyeon ^a,b   El Zaatari, Fouad A K ^a,b   Kato, Mototsugu ^a   Osato, Michael S ^a   Reddy, Rita ^a   Yamaoka, Yoshio ^a   Graham, David Y ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b MICHAEL E DEBAKEY VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; HYDROGENASE; METRONIDAZOLE; NITROREDUCTASE; OXIDOREDUCTASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM ISOLATE; GENE INACTIVATION; GENE MUTATION; HELICOBACTER PYLORI; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STRAIN DIFFERENCE;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CLONING, MOLECULAR; DNA, BACTERIAL; DRUG RESISTANCE, MICROBIAL; ESCHERICHIA COLI; FERREDOXINS; FMN REDUCTASE; GENE EXPRESSION; GENETIC COMPLEMENTATION TEST; HELICOBACTER PYLORI; HUMANS; MEMBRANE PROTEINS; METRONIDAZOLE; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; NADH, NADPH OXIDOREDUCTASES; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFORMATION, BACTERIAL;

EID: 0033913344     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.44.8.2133-2142.2000     Document Type: Article

References (42)

1. Alm, R. A., L. L. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. deJonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jinag, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397:176-180.
2. Cederbrant, G., G. Kahlmeter, and A. Ljungh. 1992. Proposed mechanism for metronidazole resistance in Hceicobacter pylori. J. Antimicrob. Chemother. 29:115-120.
3. Chang, K. C., S. W. Ho, J. C. Yang, and J. T. Wang. 1917. Isolation of a genetic locus associated with metronidazole resistance in Helicobacter pylori. Biochem. Biophys. Res. Commun. 236:785-788.
4. Crump, M., M. Gospodarowicz, and F. A. Shepherd. 1999. Lymphoma of the gastrointestinal tract. Semin. Oncol. 26:324-337.
5. Declerk, P. J., and C. J. Ranter. 1986. In vitro reductive activation of nitroimidazoles. Biochem. Pharmacol. 35:59-61.
6. Declerk, P. J., C. J. de Ranter, and G. Volckaert. 1983. Base specific interaction of reductively activated nitroimidazoles with DNA. FEBS Lett. 164: 145-148.
7. Dore, M. P., M. S. Osato, D. H. Kwon, D. Y. Graham, and F. A. K. El-Zaatari. 1998. Demonstration of unexpected antibiotic resistance of genotypically identical Helicobacter pylori isolates. Clin. Infect. Dis. 27:84-89.
8. Edwards, D. I. 1993. Nitroimidazole drugs - action and resistance mechanisms. I. Mechanisms of action. J. Antimicrob. Chemother. 31:9-20.
9. Edwards, D. I. 1993. Nitroimidazole drugs - action and resistance mechanisms. II. Mechanisms of resistance. J. Antimicrob. Chemother. 31:201-210.
10. Edwards, D. I., M. Dye, and H. Carne. 1973. The selective toxicity of antimicrobial nitroheterocyclic drugs. J. Gen. Microbiol. 76:135-145.
11. Ferrero, R. L., V. Cussac, P. Courcoux, and A. Labigne. 1992. Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange. J. Bacteriol. 174:4212-4217.
12. Ge, Z., K. Hiratsuka, and D. E. Taylor. 1995. Nucleotide sequence and mutational analysis indicate that two Helicobacter pylori genes encode a P-type ATPase and a cation-binding protein associated with copper transport. Mol. Microbiol. 15:97-106.
13. Glupczynski, Y. 1998. Antimicrobial resistance in Helicobacter pylori: a global overview. Acta Gastro-Enterol. Belg. 61:357-366.
14. Goodwin, A., D. Kersulyte, G. Sisson, S. J. O. Veldhuyzen van Zanten, D. E. Berg, and P. S. Hoffman. 1998. Metronidazole-resistance in Helicobacter pylori is due to null mutations in a gene (rdxA) that encodes an oxygen-insensitive NAD(P)H nitroreductase. Mol. Microbiol. 28:383-393.
15. Graham, D. Y., W. A. de Boer, and G. N. Tytgat. 1996. Choosing the best anti-Helicobacter pylori therapy: effect of antimicrobial resistance. Am. J. Gastroenterol. 91:1072-1076.
16. Graham, D. Y. 1998. Antibiotic resistance in Helicobacter pylori: implications for therapy. Gastroenterology 115:1272-1277.
17. Hachem, C. Y., J. E. Clarridge, D. G. Evans, and D. Y. Graham. 1995. Comparison of agar based media for primary isolation of Helicobacter pylori. J. Clin. Pathol. 48:714-716.
18. Hachem, C. Y., J. E. Clarridge, R. Reddy, and D. Y. Graham. 1996 Antimicrobial susceptibility testing of Helicobacter pylori. Comparison of E-test, broth microdilution, and disk diffusion for ampicillin, clarithromycin, and metronidazole. Diagn. Microbiol. Infect. Dis. 24:37-41.
19. Heuermann, D., and R. Haas. 1998. A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation. Mol. Gen. Genet. 257:519-528.
20. Hoffman, P. S., A. Goodwin, J. Johnsen, K. Magee, and S. J. Veldhuygen van Zanten. 1996. Metabolic activities of metronidazole-sensitive and -resistant strains of Helicobacter pylori: repression of pyruvate oxidoreductase and expression of isocitrate lyase activity correlate with resistance. J. Bacteriol. 178:4822-4829.
21. Houben, M. H., D. V. Beek, E. F. Hensen, A. J. Craen, E. A. Rauws, and G. N. Tytgat. 1999. A systematic review of Helicobacter pylori eradication therapy - the impact of antimicrobial resistance on eradication rates. Aliment. Pharmacol. Ther. 13:1047-1055.
22. Hughes, N. J., C. L. Clayton, P. A. Chalk, and D. J. Kelly. 1998. Helicobacter pylori porCDAB and oorDABC genes encode distinct pyruvate: flavodoxin and 2-oxoglutarate: acceptor oxidoreductases which mediate electron transport to NADP. J. Bacteriol. 180:1119-1128.
23. Hughes, N. J., P. A. Chalk, C. L. Clayton, and D. J. Kelly. 1995. Identification of carboxylation enzymes and characterization of a novel four-subunit pyruvate: flavodoxin oxidureductase from Helicobacter pylori. J. Bacteriol. 177: 3953-3959.
24. Jenks, P. J., R. L. Ferrero, and A. Labigne. 1999. The role of the rdxA gene in the evolution of metronidazole resistance in Helicobacter pylori. J. Antimicrob. Chemother. 43:753-758.
25. Jorgensen, M. A., J. Manos, G. L. Mendz, and S. L. Hazell. 1998. The mode of action of metronidazole in Helicobacter pylori: futile cycling or reduction? J. Antimicrob. Chemother. 41:67-75.
26. Kaihovaara, P., J. Hook-Nikanne, M. Uusi-Oukari, T. U. Kosunen, and M. Salaspuro. 1998. Flavodoxin-dependent pyruvate oxidation, acetate production and metronidazole reduction by Helicobacter pylori. J. Antimicrob. Chemother. 41:171-177.
27. Kedderis, G. L., L. S. Argenbright, and G. T. Miwa. 1988. Mechanism of reductive activation of a 5-nitroimidazole by flavoproteins: model studies with dithionite. Arch. Biochem. Biophys. 262:40-48.
28. Kwon, D. H., J. S. Woo, C. L. Perng, M. F. Go, D. Y. Graham, and F. A. K. El-Zaatari. 1998. The effect of galE gene inactivation on lipopolysaccharide profile of Helicobacter pylori. Curr. Microbiol. 37:144-148.
29. Lindmark, D. G., and M. Muller. 1976. Antitrichomonad action, mutagenicity, and reduction of metronidazole and other nitroimidazoles. Antimicrob. Agents Chemother. 10:476-482.
30. Lockerby, L., H. R. Rabin, and E. J. Laishley. 1985. Role of the phosphoroclastic reaction of Clostridium pasteurianum in the reduction of metronidazole. Antimicrob. Agents Chemother. 27:863-867.
31. Megraud, F. 1994. Helicobacter pylori resistance to antibiotics, p. 570-583. In R. H. Hunt and G. N. J. Tytgat (ed.). Helicobacter pylori: basic mechanisms tio clinical cure. Kluwer Academic Publishers, Dordrecht, The Netherlands.
32. Megraud, F. 1998. Antibiotic resistance in Helicobacter pylori infection. Br. Med. Bull. 54:207-216.
33. Muller, M. 1983. Mode of action of metronidazole on anaerobic bacteria and protozoa. Surgery 93:165-171.
34. Parsonnet, J. 1998. Helicobacter pylori. Infect. Dis. Clin. N. Am. 12:185-197.
35. Parsonnet, J. 1998. Helicobacter pylori: the size of the problem. Gut 43(Suppl. 1):S6-S9.
36. Realdi, G., M. P. Dore, A. Piana, A. Atzei, M. Carta, L. Cugia, A. Manca, B. M. Are, G. Massarelli, I. Mura, A. Maida, and D. Y. Graham. 1999. Pretreatment antibiotic resistance in Helicobacter pylori infection: results of three randomized controlled studies. Helicobacter 4:106-112.
37. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
38. Smith, M. A., and D. I. Edwards. 1997. Oxygen scavenging, NADH oxidase and metronidazole resistance in Helicobacter pylori. J. Antimicrob. Chemother. 39:347-353.
39. Tomb, J. F., O. White, A. R. Kerlavage, R. A. Clayton, G. G. Sutton, R. D. Fleischmann, K. A. Ketchum, H. P. Klenk, S. Gill, B. A. Dougherty, K. Nelson, J. Quackenbush, L. Zhou, E. F. Kirkness, S. Peterson, B. Loftus, D. Richardson, R. Dodson, H. G. Khalak, A. Glodek, K. McKenny, L. M. Fitzegerald, N. M. Lee, M. D. Adams, E. K. Hickey, D. E. Berg, J. D. Gocayne, T. R. Utterback, J. D. Peterson, J. M. Kelley, M. D. Cotton, J. M. Weidman, C. Fujii, C. Bowman, L. Watthey, E. Wallin, W. S. Hays, M. Borodovsky, P. D. Karp, H. O. Smith, C. M. Fraser, and J. C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388:539-547.
40. Van der Wouden, E. J., J. C. Thijs, A. A. van Zwet, W. J. Sluiter, and J. H. Kleibeuker. 1999. The influence of in vitro nitroimidazole resistance on the efficacy of nitroimidazole-containing anti-Helicobacter pylori regimens: a meta-analysis. Am. J. Gastroenterol. 94:1751-1759.
41. Wang, Y., K. P. Roos, and D. E. Taylor. 1993. Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. J. Gen. Microbiol. 139:2485-2493.
42. Weel, J. F., R. W. van der Hulst, Y. Gerrits, G. N. Tytgat, A. van der Ende, and J. Dankert. 1996. Heterogeneity in susceptibility to metronidazole among Helicobacter pylori isolates from patients with gastritis or peptic ulcer disease. J. Clin. Microbiol. 34:2158-2162.