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Reducing the complexity of a peptide mixture derived from the digestion of a complex protein sample by the reversible affinity capture of cysteinyl peptides prior to LC-MS/MS.
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An extensive characterization of the yeast nuclear pore complex accomplished by isolation of the nuclear pore complex (NPC) followed by chromatography and SDS-PAGE of the fractions and identification of all protein bands. An extreme case of the signal to noise issue, as the vast majority of identified proteins were those in transit through and not part of the NPC structure.
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Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol. 148:2000;635-651. An extensive characterization of the yeast nuclear pore complex accomplished by isolation of the nuclear pore complex (NPC) followed by chromatography and SDS-PAGE of the fractions and identification of all protein bands. An extreme case of the signal to noise issue, as the vast majority of identified proteins were those in transit through and not part of the NPC structure.
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