P2X1 receptor membrane redistribution and down-regulation visualized by using receptor-coupled green fluorescent protein chimeras

Neuropharmacology

Volumn 39, Issue 11, 2000, Pages 2054-2066

  Dutton, J L ^a   Poronnik, P ^b   Li, G H ^a   Holding, C A ^a   Worthington, R A ^c   Vandenberg, R J ^d   Cook, D I ^b   Barden, J A ^c   Bennett, M R ^a  

^a Institute for Biomedical Research   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

^c Protein Receptor Struct Laboratory   (Australia)

^d University of Sydney   (Australia)

Author keywords

ATP; Ionotropic; P2X1 GFP; Purinergic; Receptors; Replication deficient adenovirus

Indexed keywords

ADENOSINE TRIPHOSPHATE; GREEN FLUORESCENT PROTEIN; PURINE P2X RECEPTOR; PURINERGIC RECEPTOR STIMULATING AGENT; RECEPTOR SUBTYPE;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CHIMERA; CONTROLLED STUDY; INTERNALIZATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RECEPTOR DOWN REGULATION; SYNAPSE; XENOPUS;

ADENOSINE TRIPHOSPHATE; ADENOVIRIDAE; ANIMALS; CELL LINE; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MEMBRANE POTENTIALS; RECEPTORS, PURINERGIC P2; RECOMBINANT FUSION PROTEINS; TRANSCRIPTION, GENETIC; TRANSFECTION; XENOPUS;

EID: 0033841948     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(00)00058-7     Document Type: Article

References (43)

1. Baenziger J.E., Chew J.P. Desensitization of the nicotinic acetylcholine receptor mainly involves a structural change in solvent-accessible regions of the polypeptide backbone. Biochemistry. 36:1997;3617-3624.
2. Barak L.S., Ferguson S.S., Zhang J., Martenson C., Meyer T., Caron M.G. Internal trafficking and surface mobility of a functionally intact beta2-adrenergic receptor-green fluorescent protein conjugate. Mol. Pharmacol. 51:1997;177-184.
3. Barden J.A., Cottee L.J., Bennett M.R. Vesicle-associated proteins and P2X receptor clusters at single sympathetic varicosities in mouse vas deferens. J. Neurocytol. 28:1999;469-480.
4. Britt J.C., Brenner H.R. Rapid drug application resolves two types of nicotinic receptors on rat sympathetic ganglion cells. Pflügers Arch. Eur. J. Physiol. 434:1997;38-48.
5. Buell G., Collo G., Rassendren F. P2X receptors: an emerging channel family. Eur. J. Neurosci. 8:1996;2221-2228.
6. Bufler J., Heckmann M., Jahn K., Franke C. Distribution of desensitization time constants of mouse embryonic-like nicotinic and homomeric GluR6 glutamate receptor channels. Neurosci. Lett. 221:1997;173-176.
7. 2+-dependent modulation of ATP-gated P2X receptors in nociceptors. Neuropharmacology. 36:1997;1303-1308.
8. Cremaschi D., Porta C., Ghirardelli R., Manzoni C., Caremi I. Endocytosis inhibitors abolish the active transport of polypeptides in the mucosa of the nasal upper concha of the rabbit. Biochim. Biophys. Acta. 1280:1996;27-33.
9. Dutton J.L., Hansen M.A., Balcar V.J., Barden J.A., Bennett M.R. Development of P2X receptor clusters on smooth muscle cells in relation to nerve varicosities in the rat urinary bladder. J. Neurocytol. 28:1999;3-15.
10. Evans R.J., Lewis C., Buell G., Valera S., North R.A., Surprenant A. Pharmacological characterization of heterologously expressed ATP-gated cation channels (P2X purinoceptors). Mol. Pharmacol. 48:1995;178-183.
11. Evans R.J., Lewis C., Virginio C., Lundstrom K., Buell G., Surprenant A., North R.A. Ionic permeability of, and divalent cation effects on, two ATP-gated cation channels (P2X receptors) expressed in mammalian cells. J. Physiol. 496:1996;413-422.
12. Feng G., Steinbach J.H., Sanes J.R. Rapsyn clusters neuronal acetylcholine receptors but is inessential for formation of an interneuronal cholinergic synapse. J. Neurosci. 18:1998;4166-4176.
13. Fonseca M.I., Button D.C., Brown R.D. Agonist regulation of alpha 1B-adrenergic receptor subcellular distribution and function. J. Biol. Chem. 270:1995;8902-8909.
14. Froehner S.C., Luetje C.W., Scotland P.B., Patrick J. The postsynaptic 34K protein clusters muscle nicotinic acetylcholine receptors in Xenopus oocytes. Neuron. 5:1990;403-410.
15. Glass D.J., Yancopoulos G.D. Sequential roles of agrin, MuSK and rapsyn during neuromuscular junction formation. Curr. Opin. Neurobiol. 7:1997;379-384.
16. Grassi F., Palma E., Mileo A.M., Eusebi F. The desensitization of the embryonic mouse muscle acetylcholine receptor depends on the cellular environment. Pflügers Arch. Eur. J. Physiol. 430:1995;787-794.
17. 1-receptor clusters on smooth muscle cells in relation to nerve varicosities in the rat urinary bladder. J. Neurocytol. 27:1998;529-539.
18. Hansen M.A., Dutton J.L., Balcar V.J., Barden J.A., Bennett M.R. P2X (purinergic) receptor distributions in rat blood vessels. J. Auton. Nerv. Sys. 75:1999;147-155.
19. He T.C., Zhou S., da Costa L.T., Yu J., Kinzler K.W., Vogelstein B. A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. U. S. A. 95:1998;2509-2514.
20. Heck D.A., Bylund D.B. Differential down-regulation of alpha-2 adrenergic receptor subtypes. Life Sci. 62:1998;2467-2472.
21. Hoffman P.W., Ravindran A., Huganir R.L. Role of phosphorylation in desensitization of acetylcholine receptors expressed in Xenopus oocytes. J. Neurosci. 14:1994;4185-4195.
22. Kallal L., Gagnon A.W., Penn R.B., Benovic J.L. Visualization of agonist-induced sequestration and down-regulation of a green fluorescent protein-tagged beta2-adrenergic receptor. J. Biol. Chem. 273:1998;322-328.
23. Khiroug L., Sokolova E., Giniatullin R., Afzalov R., Nistri A. Recovery from desensitization of neuronal nicotinic acetylcholine receptors of rat chromaffin cells is modulated by intracellular calcium through distinct second messengers. J. Neurosci. 18:1998;2458-2466.
24. Kirsch J., Betz H. Glycine-receptor activation is required for receptor clustering in spinal neurons. Nature. 392:1998;717-720.
25. Kneen M., Farinas J., Li Y., Verkman A.S. Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74:1998;1591-1599.
26. Lester R.A., Dani J.A. Acetylcholine receptor desensitization induced by nicotine in rat medial habenula neurones. J. Neurophysiol. 74:1995;195-206.
27. Maimone M.M., Merlie J.P. Interaction of the 43 kD postsynaptic protein with all subunits of the muscle nicotinic acetylcholine receptor. Neuron. 11:1993;53-66.
28. North R.A., Barnard E.A. Nucleotide receptors. Curr. Opin. Neurobiol. 7:1997;346-357.
29. Parker K.E. Modulation of ATP-gated non-selective cation channel (P2X1 receptor) activation and desensitization by the actin cytoskeleton. J. Physiol. 510:1998;19-25.
30. Pfeiffer F., Graham D., Betz H. Purification by affinity chromatography of the glycine receptor of rat spinal cord. J. Biol. Chem. 257:1982;9389-9393.
31. Phillips W.D., Bennett M.R. Elimination of distributed synaptic acetylcholine receptor clusters on developing avian fast-twitch fibers accompanies loss of polyneuronal innervation. J. Neurocytol. 16:1987;785-797.
32. Phillips W.D., Bennett M.R. The distribution of intracellular acetylcholine receptors and nuclei in developing avian fast-twitch muscle fibres during synapse elimination. J. Neurocytol. 18:1989;241-255.
33. Phillips W.D., Lai K., Bennett M.R. Spatial distribution and size of acetylcholine receptor clusters determined by motor nerves in developing chick muscles. J. Neurocytol. 14:1985;309-325.
34. 2+ signalling in epithelial cells. Cell Calcium. 24:1998;97-103.
35. Ramarao M.K., Cohen J.B. Mechanism of nicotinic acetylcholine receptor cluster formation by rapsyn. Proc. Natl. Acad. Sci. U. S. A. 95:1998;4007-4012.
36. Strasser R.H., Stiles G.L., Lefkowitz R.J. Translocation and uncoupling of the beta-adrenergic receptor in rat lung after catecholamine promoted desensitization in vivo. Endocrinology. 115:1984;1392-1400.
37. 7). Science. 272:1996;735-738.
38. Yu S.S., Lefkowitz R.J., Hausdorff W.P. Beta-adrenergic receptor sequestration. A potential mechanism of receptor resensitization. J. Biol. Chem. 268:1993;337-341.
39. Valenta D.C., Downing J.E., Role L.W. Peptide modulation of ACh receptor desensitization controls neurotransmitter release from chicken sympathetic neurones. J. Neurophysiol. 69:1993;928-942.
40. Valera S., Hussy N., Evans R.J., Adami N., North R.A., Surprenant A., Buell G. A new class of ligand-gated ion channel defined by P2X receptor for extracellular ATP. Nature. 371:1994;516-519.
41. Vulchanova L., Arvidsson U., Riedl M., Wang J., Buell G., Surprenant A., North R.A., Elde R. Differential distribution of two ATP-gated channels (P2X receptors) determined by immunocytochemistry. Proc. Natl. Acad. Sci. U. S. A. 93:1996;8063-8067.
42. Werner P., Seward E.P., Buell G.N., North R.A. Domains of P2X receptors involved in desensitization. Proc. Natl. Acad. Sci. U. S. A. 93:1996;15485-15490.
43. Worthington R.A., Dutton J.L., Poronnik P., Bennett M.R., Barden J.A. Localisation of P2X receptors in HSG and HEK cells by SDS-PAGE/Western blotting and immunofluorescence. Electrophoresis. 20:1999;2065-2070.