Flow cytometry and the single cell in phycology

Journal of Phycology

Volumn 36, Issue 4, 2000, Pages 628-644

  Collier, Jackie L ^a  

^a Research Rensselaer Polytechnic Institute   (United States)

Author keywords

Flow cytometer; Fluorescence; Light scatter; Molecular probes; Photosynthesis; Phytoplankton; Pigments

Indexed keywords

FLOW CYTOMETRY; PHYCOLOGY; PICOPHYTOPLANKTON; PROCESS OPTIMIZATION; WATER SAMPLING;

FLOW CYTOMETRY; PHYTOPLANKTON;

EID: 0033834509     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1529-8817.2000.99215.x     Document Type: Review

References (159)

1. Ackleson, S. G. & Spinrad, R. W. 1988. Size and refractive index of individual marine participates: a flow cytometric approach. Appl. Optics 27:1270-7.
2. Amann, R. I., Ludwig, W. & Schleifer, K. -H. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-69.
3. Anderson, D. M., Kulis, D. M., Keafer, B. A. & Berdalet, E. 1999. Detection of the toxic dinoflagellate Alexandrium fundyense (Dinophyceae) with oligonucleotide and antibody probes: variability in labeling intensity with physiological condition. J. Phycol. 35:870-83.
4. Armbrust, E. V., Chisholm, S. W. & Olson, R. J. 1990. Role of light and the cell cycle on the induction of spermatogenesis in a centric diatom. J. Phycol. 26:470-78.
5. Ault, K. A. 1991. Calibration and quantitative analysis. In Demers, S. [Ed.] Particle Analysis in Oceanography. Springer-Verlag, Berlin, pp. 47-58.
6. Balfoort, H. W., Berman, T., Maestrini, S. Y., Wenzel, A. & Zohary, T. 1992. Flow cytometry: instrumentation and application in phytoplankton research. Hydrobiologia 238:89-97.
7. Binder, B. J. & Liu, Y. C. 1998. Growth rate regulation of rRNA content of a marine Synechococcus (Cyanobacterium) strain. Appl. Environ. Microbiol. 64:3346-51.
8. Bloodgood, R. A., Salomonsky, N. L. & Reinhart, F. D. 1987. Use of carbohydrate probes in conjunction with fluorescence-activated cell sorting to select mutant cell lines of Chlamydomonas with defects in cell surface glycoproteins. Exp. Cell Res. 173:572-85.
9. Bougrier, S., Hawkins, A. J. S. & Heral, M. 1997. Preingestive selection of different microalgal mixtures in Crassostrea gigas and Mytilus edulis, analyzed by flow cytometry. Aquaculture 150:123-34.
10. Bruschi, C. V. & Chuba, P. J. 1988. Nonselective enrichment for yeast adenine mutants by flow cytometry. Cytometry 9:60-7.
11. Brussaard, C. P. D., Thyrhaug, R., Marie, D. & Bratbak, G. 1999. Flow cytometric analyses of viral infection in two marine phytoplankton species, Micromonas pusilla (Prasinophyceae) and Phaeocystis pouchetii (Prymnesiophyceae). J. Phycol. 35:941-8.
12. Buma, A. G. J., Van Hannen, E. J., Roza, L., Veldhuis, M. J. W. & Gieskes, W. W. C. 1995. Monitoring ultraviolet-B-induced DNA damage in individual diatom cells by immunofluorescent thymine dimer detection. J. Phycol. 31:314-21.
13. Campbell, J. W. & Yentsch, C. M. 1989a. Variance within homogeneous phytoplankton populations, I: theoretical framework for interpreting histograms. Cytometry 10:587-95.
14. _ 1989b. Variance within homogeneous phytoplankton populations, II: analysis of clonal cultures. Cytometry 10:596-604.
15. Campbell, J. W., Yentsch, C. M. & Cucci, T. L. 1989. Variance within homogeneous phytoplankton populations, III: analysis of natural populations. Cytometry 10:605-11.
16. Campbell, I. & Vaulot, D. 1993. Photosynthetic picoplankton community structure in the subtropical North Pacific Ocean near Hawaii (Station ALOHA). Deep Sea Res. 40:2043-60.
17. Carre, I. A. & Edmunds, L. N., Jr. 1993. Oscillator control of cell division in Euglena: cyclic AMP oscillations mediate the phasing of the cell division cycle by the circadian clock. J. Cell Sci. 104:1163-73.
18. Cavender-Bares, K. K., Frankel, S. L. & Chisholm, S. W. 1998. A dual sheath flow cytometer for shipboard analyses of phytoplankton communities from the oligotrophic oceans. Limnol. Oceanogr. 43:1383-8.
19. Cavender-Bares, K. K., Mann, E. L., Chisholm, S. W., Ondrusek, M. E. & Bidigare, R. R. 1999. Differential response of equatorial Pacific phytoplankton to iron fertilization. Limnol. Oceanogr. 44:237-46.
20. Chen, F., Binder, B. & Hodson, R. E. 2000. Flow cytometric detection of specific gene expression in prokaryotic cells using in situ RT-PCR. FEMS Microbiol. Lett. 184:291-295.
21. Chisholm, S. W., Armbrust, E. V. & Olson, R. J. 1986. The individual cell in phytoplankton ecology: cell cycles and applications of flow cytometry. Can. Bull. Fish. Aquat. Sci. 214:343-69.
22. Chisholm, S. W., Olson, R. J., Zettler, E. R., Goericke, R., Waterbury, J. B. & Welschmeyer, N. A. 1988. A novel free-living prochlorophyte abundant in the oceanic euphotic zone. Nature (Lond.). 334:340-3.
23. Cid, A., Fidalgo, P., Herrero, C. & Abalde, J. 1996. Toxic action of copper on the membrane system of a marine diatom measured by flow cytometry. Cytometry 25:32-6.
24. Claustre, H., Morel, A., Babin, M., Cailliau, C., Marie, D., Marty, J.-C., Tailliez, D. & Vaulot, D. 1999. Variability in particle attenuation and chlorophyll fluorescence in the tropical Pacific: scales, patterns, and biogeochemical implications. J. Geophys. Res. 104:3401-22.
25. Collier, J. L. & Campbell, L. 1999. Flow cytometry in molecular aquatic ecology. Hydrobiologia 401:33-53.
26. Corzo, A., Jimenez-Gomez, F., Gordillo, F. J. L., Garcia-Ruiz, R. & Niell, F. X. 1999. Synechococcus and Prochlorococcus-like populations detected by flow cytometry in a eutrophic reservoir in summer. J. Plankton Res. 21:1575-81.
27. Corzo, A., Vergara, J. J. & Garcia-Jimenez, M. C. 1995. Isolation and flow cytometric characterization of protoplasts from marine macroalgae. J. Phycol. 31:1018-26.
28. Courties, C., Vaquer, A., Troussellier, M., Lautier, J., Chretiennot-Dinet, M. J., Neveux, J., Machado, C. & Claustre, H. 1994. Smallest eukaryotic organism. Nature (Lond.). 370:255.
29. Coury, D. A., Naganuma, T., Polne-Fuller, M. & Gibor, A. 1993. Protoplasts of Gelidium robustum (Rhodophyta). Hydrobiologia 260/ 261:421-7.
30. Cucci, T. L., Shumway, S. E., Brown, W. S. & Newell, C. R. 1989. Using phytoplankton and flow cytometry to analyze grazing by marine organisms. Cytometry 10:659-69.
31. Cunningham, A. 1990a. A low-cost, portable flow cytometer specifically designed for phytoplankton analysis. J. Plankton Res. 12:149-60.
32. _ 1990b. Fluorescence pulse shape as a morphological indicator in the analysis of colonial microalgae by flow cytometry. J. Microbiol. Methods 11:27-36.
33. Cunningham, A. & Buonnacorsi, G. A. 1992. Narrow-angle forward light scattering from individual algal cells: implications for size and shape discrimination in flow cytometry. J. Plankton Res. 14:223-34.
34. Davey, H. M & Kell, D. B. 1996. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiol. Rev. 60:641-96.
35. Demers, S., Davis, K. & Cucci, T. L. 1989. A flow cytometric approach to assessing the environmental and physiological status of phytoplankton. Cytometry 10:644-52.
36. Demers, S., Kim, J., Legendre, P. & Legendre, L. 1992. Analyzing multivariate flow cytometric data in aquatic sciences. Cytometry 13:291-8.
37. Dorsey, J., Yentsch, C. M., Mayo, S. & McKenna, C. 1989. Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae. Cytometry 10:622-8.
38. Druehl, L. D., Robertson, B. R. & Button, D. K. 1989. Characterizing and sexing laminarialean meiospores by flow cytometry. Mar. Biol. 101:451-6.
39. Dubelaar, G. B. J., Greonewegen, A. C., Stokdijk, W., van den Engh, G. J. & Visser, J. W. M. 1989. Optical plankton analyser: a flow cytometer for plankton analysis, II: specifications. Cytometry 10:529-39.
40. Dubelaar, G. B. J. & van der Reijden, C. S. 1995. Size distributions of Microcystis aeruginosa colonies: a flow cytometric approach. Water Sci. Technol. 32:171-6.
41. Dubelaar, G. B. J., Visser, J. W. M. & Douze, M. 1987. Anomalous behaviour of forward and perpendicular light scattering of a cyanobacterium owing to intracellular gas vacuoles. Cytometry 8:405-12.
42. DuRand, M. D. & Olson, R. J. 1996. Contributions of phytoplankton light scattering and cell concentration changes to diel variations in beam attenuation in the equatorial Pacific from flow cytometric measurements of pico- ultra- and nanoplankton. Deep Sea Res. II, 43: 891-906.
43. _ 1998. Diel patterns in optical properties of the chlorophyte Nannochloris sp.: relating individual cell to bulk measurements. Limnol. Oceanogr. 43:1107-18.
44. Durand, R. E. 1994. Calibration of flow cytometer detector systems. Methods Cell Biol. 42:597-604.
45. Dusenberry, J. A. & Frankel, S. L. 1994. Increasing the sensitivity of a FACScan flow cytometer to study oceanic picoplankton. Limnol. Oceanogr. 39:206-9.
46. Dusenberry, J. A., Olson, R. J. & Chisholm, S. W. 1999. Frequency distributions of phytoplankton single-cell fluorescence and vertical mixing in the surface ocean. Limnol. Oceanogr. 44:431-5.
47. Dyhrman, S. T. & Palenik, B. 1997. The identification and purification of a cell-surface alkaline phosphatase from the dinoflagellate Prorocentrum minimum (Dinophyceae). J. Phycol. 33: 602-12.
48. Edvardsen, B. & Vaulot, D. 1996. Ploidy analysis of the two motile forms of Chrysochromulina polylepis (Prymnesiophyceae). J. Phycol. 32:94-102.
49. Faber, M. J., Smith, L. M. J., Boermans, H. J., Stevenson, G. R., Thompson, D. G. & Solomon, K. R. 1997. Cryopreservation of fluorescent marker-labeled algae (Selenastrum capricornutum) for toxicity testing using flow cytometry. Environ. Toxicol. Chem. 16:1059-67.
50. Fahnenstiel, G. L. & Carrick, H. J. 1992. Phototrophic picoplankton in Lakes Huron and Michigan: abundance, distribution, composition, and contribution to biomass and production. Can. J. Fish. Aquatic Sci. 49:379-88.
51. Falchuk, K. H., Krishan, A. & Vallee, B. L. 1975. DNA distribution in the cell cycle of Euglena gracilis. Cytofluorometry of zinc deficient cells. Biochemistry 14:3439-44.
52. Fouchet, P., Jayat, C., Hechard, Y., Ratinaud, M. -H. & Frelat, G. 1993. Recent advances of flow cytometry in fundamental and applied microbiology. Biol. Cell. 78:95-109.
53. Frankel, S. L., Binder, B. J., Chisholm, S. W. & Shapiro, H. M. 1990. A high-sensitivity flow cytometer for studying picoplankton. Limnol. Oceanogr. 35:1164-9.
54. Furuya, K. & Li, W. K. W. 1992. Evaluation of photosynthetic capacity in phytoplankton by flow cytometric analysis of DCMU-enhanced chlorophyll fluorescence. Mar. Ecol. Prog. Ser. 88: 279-87.
55. Galbraith, D. W. 1994. Flow cytometry and sorting of plant protoplasts and cells. Methods Cell Biol. 42:539-61.
56. Galbraith, D. W., Harkins, K. R. & Jefferson, R. A. 1988. Flow cytometric characterization of the chlorophyll contents and size distributions of plant protoplasts. Cytometry 9:75-83.
57. Galbraith, D. W., Lambert, G. M., Grebenok, R. J. & Sheen, J. 1995. Flow cytometric analysis of transgene expression in higher plants: green-fluorescent protein. Methods Cell Biol. 50:3-14.
58. Gilbert, P. M., Kana, T. M., Olson, R. J., Kirchman, D. L. & Alberte, R. S. 1986. Clonal comparisons of growth and photosynthetic responses to nitrogen availability in marine Synechococcus spp. J. Exp. Mar. Biol. Ecol. 101:199-208.
59. Haugen, E. M., Cucci, T. L., Yentsch, C. M. & Shapiro, L. P. 1987. Effects of flow cytometric analysis on morphology and viability of fragile phytoplankton. Appl. Environ. Microbiol. 53:2677-9.
60. Haugland, R. P. 1994. Spectra of fluorescent dyes used in flow cytometry. Methods Cell Biol. 42:641-63.
61. _ 1996. Handbook of Fluorescent Probes and Research Chemicals. 6th Ed. Molecular Probes Inc, Eugene, OR, 679 pp.
62. Hofstraat, J. W., van Zeijl, W. J. M., de Vreeze, M. E. J., Peeters, J. C. H., Peperzak, L., Colijn, F. & Rademaker, T. W. M. 1994. Phytoplankton monitoring by flow cytometry. J. Plankton Res. 16:1197-224.
63. Hutter, K.-J. & Eipel, H. E. 1978. Flow cytometric determinations of cellular substances in algae, bacteria, moulds and yeasts. Antonie I Leeuwenhoek 44:269-82.
64. _ 1979. Microbial determinations by flow cytometry. J. Gen. Microbiol. 113:369-75.
65. Jacquet, S., Lennon, J. -F., Marie, D. & Vaulot, D. 1998. Picoplankton population dynamics in coastal waters of the northwestern Mediterranean Sea. Limnol. Oceanogr. 43:1916-31.
66. Jonker, R. R., Meulemans, J. T., Dubelaar, G. B. J., Wilkins, M. F. & Ringelberg, J. 1995. Flow cytometry: a powerful tool in analysis of biomass distributions in phytoplankton. Water Sci. Technol. 32:177-82.
67. Keij, J. F., Groenewegen, A. C. & Visser, J. W. M. 1994. High-speed photodamage cell sorting: an evaluation of the ZAPPER prototype. Methods Cell Biol. 42:371-86.
68. Kerker, M. 1983. Elastic and inelastic light scattering in flow cytometry. Cytometry 4:1-10.
69. Kirk, J. T. O. 1994. Light and Photosynthesis in Aquatic Ecosystems. Cambridge University Press, Cambridge, 509 pp.
70. La Roche, J., Boyd, P. W., McKay, R. M. L. & Geider, R. J. 1996. Flavodoxin as an in situ marker for iron stress in phytoplankton. Nature (Lond.) 382:802-5.
71. Lange, M., Guillou, L., Vaulot, D., Simon, N., Amann, R. I., Ludwig, W. & Medlin, L. K. 1996. Identification of the class Prymnesiophyceae and the genus Phaeocystis with ribosomal RNA-targeted nucleic acid probes detected by flow cytometry. J. Phycol. 32:858-68.
72. Leary, J. F. 1994. Strategics for rare cell detection and isolation. Methods Cell Biol. 42:331-58.
73. Lepesteur, M., Martin, J. M. & Fleury, A. 1993. A comparative study of different preservation methods for phytoplankton cell analysis by flow cytometry. Mar. Ecol. Prog. Ser. 93:55-63.
74. Leveson, A. C. & Wong, J. T. Y. 1999. PCNA-like proteins in dinoflagellates. J. Phycol. 35: 798-805.
75. Li, W. K. W. 1989. Shipboard analytical flow cytometry of oceanic ultraphytoplankton. Cytometry 10:564-79.
76. _ 1990. Bivariate and trivariate analysis in flow cytometry: phytoplankton size and fluorescence. Limnol. Oceanogr. 35:1356-68.
77. _ 1994. Primary production of prochlorophytes, cyanobacteria, and eucaryotic ultraphytoplankton: measurements from flow cytometric sorting. Limnol. Oceanogr. 39:169-75.
78. _ 1995. Composition of ultraphytoplankton in the central North Atlantic. Mar. Ecol. Prog. Ser. 122:1-8.
79. _ 1997. Cytometric diversity in marine ultraphytoplankton. Limnol. Oceanogr. 42:874-80.
80. Lindell, D., Padan, E. & Post, A. F. 1998. Regulation of ntcA expression and nitrite uptake in the marine Synechococcus sp. Strain WH 7803. J. Barteriol. 180:1878-86.
81. Lipschultz, F. 1995. Nitrogen-specific uptake rates of marine phytoplankton isolated from natural populations of particles by flow cytometry. Mar. Ecol. Prog. Ser. 123:245-58.
82. Lin, H., Campbell, L. & Landry, M. R. 1995. Growth and mortality rates of Prochlorococcus and Synechococcus measured with a selective inhibitor technique. Mar. Ecol. Prog. Ser. 116:277-87.
83. Lucretti, S. & Dolezel, J. 1995. Cell cycle synchronization, chromosome isolation, and flow-sorting in plants. Methods Cell Biol. 50:61-83.
84. Maeda, H., Kawai, A. & Tilzer, M. M. 1992. The water bloom of Cyanobacterial picoplankton in Lake Biwa, Japan. Hydrobiologia 248:93-103.
85. Marie, D., Brussaard, C. P. D., Partensky, F., & Vaulot, D. 1999a. Flow cytometric analysis of phytoplankton, bacteria, and viruses. In Robinson, J. P. [Ed.] Current Protocols in Cytometry. John Wiley & Sons, Inc., New York, pp. 11.11.1-11.11.15.
86. Marie, D., Brussaard, C. P. D., Thyrhaug, R., Bratbak, G. & Vaulot, D. 1999b. Enumeration of marine viruses in culture and natural samples by flow cytometry. Appl. Environ. Microbiol. 65: 45-32.
87. Marie, D., Partensky, F., Jacquet, S. & Vaulot, D. 1997. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl. Environ. Microbiol. 63:186-93.
88. McAuley, P. J. & Muscatine, L. 1986. The cell cycle of symbiotic Chlorella. IV. DNA content of algae slowly increases during host starvation of green hydra. J. Cell Sci. 85:73-84.
89. Moore, L. R., Rocap, G. & Chisholm, S. W. 1998. Physiology and molecular phylogeny of coexisting Prochlorococcus ecotypes. Nature (Lond.) 393:464-7.
90. Mori, T., Binder, B. & Johnson, C. H. 1996. Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours. Proc. Natl. Acad. Sci. USA 93:10183-8.
91. Neale, P. J., Cullen, J. J. & Yentsch, C. M. 1989. Bio-optical inferences from chlorophyll a fluorescence: what kind of fluorescence is measured in flow cytometry? Limnol. Oceanogr. 34:1739-48.
92. Olson, R. J., Chekalyuk, A. M. & Sosik, H. M. 1996. Phytoplankton photosynthetic characteristics from fluorescence induction assays of individual cells. Limnol. Oceanogr. 41:1253-63.
93. Olson, R. J., Chisholm, S. W., Zettler, E. R., Altabet, M. A. & Dusenberry, J. A. 1990a. Spatial and temporal distributions of prochlorophyte picoplankton in the North Atlantic Ocean. Deep Sea Res. 37:1033-51.
94. Olson, R. J., Chisholm, S. W., Zettler, E. R. & Armbrust, E. V. 1990b. Pigments, size, and distribution of Synechococcus in the North Atlantic and Pacific Oceans. Limnol. Oceanogr. 35:45-58.
95. Olson, R. J., Frankel, S. L., Chisholm, S. W. & Shapiro, H. M. 1983. An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton: chlorophyll and DNA distributions. J. Exp. Mar. Biol. Ecol. 68:129-44.
96. Olson, R. J., Vaulot, D. & Chisholm, S. W. 1986. Effects of environmental stresses on the cell cycle of two marine phytoplankton species. Plant Physiol. 80:918-25.
97. Olson, R. J. & Zettler, E. R. 1995. Potential of flow cytometry for "pump and probe" fluorescence measurements of phytoplankton photosynthetic characteristics. Limnol. Oceanogr. 40:816-20.
98. Olson, R. J., Zettler, E. R. & Anderson, O. K. 1989. Discrimination of eukaryotic phytoplankton cell types from light scatter and autofluorescence properties measured by flow cytometry. Cytometry 10:636-43.
99. Olson, R. J., Zettler, E. R., Chisholm, S. W. & Dusenberry, J. A. 1991. Advances in oceanography through flow cytometry. In Demers, S. [Ed.] Particle Analysis in Oceanography. Springer-Verlag, Berlin, pp. 351-99.
100. Olson, R. J., Zettler, E. R. & DuRand, M. D. 1993. Phytoplankton analysis using flow cytometry. In Kemp, P. F. [Ed.] Handbook of Methods in Aquatic Microbial Ecology. CRC Press, Inc., Boca Raton, FL, pp. 175-86.
101. Orellana, M. V. & Perry, M. J. 1995. Optimization of an immunofluorescent assay of the internal enzyme ribulose-1,5-bisphosphate carboxylase (Rubisco) in single phytoplankton cells. J. Phycol. 31:785-94.
102. Owens, T. G. 1991. Energy transformation and fluorescence in photosynthesis. In Demers, S. [Ed.] Particle Analysis in Oceanography. Springer-Verlag, Berlin Heidelberg, pp. 101-37.
103. Paau, A. S., Cowles, J. R., Oro, J., Bartel, A. & Hungerford, E. 1979. Separation of algal mixtures and bacterial mixtures with flow-microfluorometer using chlorophyll and ethidium bromide fluorescence. Arch. Microbiol. 120:271-3.
104. Paau, A. S., Oro, J. & Cowles, J. R. 1978. Application of flow microfluorometry to the study of algal cells and isolated chloroplasts. J. Exp. Bot. 29:1011-20.
105. Palenik, B. 1994. Cyanobacterial community structure as seen from RNA polymerase gene sequence analysis. J. Bacteriol. 60:3212-9.
106. Palenik, B. & Koke, J. A. 1995. Characterization of a nitrogen-regulated protein identified by cell surface biotinylation of a marine phytoplankton. Appl. Environ. Microbiol. 61:3311-5.
107. Partensky, F., Blanchot, J., Lantoine, F., Neveux, J. & Marie, D. 1996. Vertical structure of picophytoplankton at different trophic sites of the tropical northeastern Atlantic Ocean. Deep Sea Res. I 43:1191-213.
108. Partensky, F., Hess, W. R. & Vaulot, D. 1999. Prochlorococcus, a marine photosynthetic prokaryote of global significance. Microbiol. Mol. Biol. Rev. 63:106-27.
109. Peeters, J. C. H., Dubelaar, G. B. J., Ringelberg, J. & Visser, J. W. M. 1989. Optical plankton analyser: a flow cytometer for plankton analysis, I: design considerations. Cytometry 10:522-8.
110. Perry, M. J. & Porter, S. M. 1989. Determination of the cross-section absorption coefficient of individual phytoplankton cells by analytical flow cytometry. Limnol. Oceanogr. 34:1727-38.
111. Phinney, D A. & Cucci, T. L. 1989. Flow cytometry and phytoplankton. Cytometry 10:511-21.
112. Pile, A. J., Patterson, M. R., Savarese, M., Chernykh, V. I. & Fialkov, V. A. 1997. Trophic effects of sponge feeding within Lake Baikal's littoral zone. 2. Sponge abundance, diet, feeding efficiency, and carbon flux. Limnol. Oceanogr. 42:178-84.
113. Potter, D., Lajeunesse, T. C., Saunders, G. W. & Anderson, R. A. 1997. Convergent evolution masks extensive biodiversity among marine coccoid picoplankton. Biodiv. Conserv. 6:99-107.
114. Powell, K. T. & Weaver, J. C. 1990. Gel microdroplets and flow cytometry: rapid determination of antibody secretion by individual cells within a cell population. Biotechnology 8:333-7.
115. Price, B. J., Kollman, V. H. & Salzman, G. C. 1978. Light-scatter analysis of microalgae. Correlation of scatter patterns from pure and mixed asynchronous cultures. Biophys. J. 22:29-36.
116. Reckermann, M. & Veldhuis, M. J. W. 1997. Trophic interactions between picophytoplankton and micro- and nanozooplankton in the western Arabian Sea during the NE monsoon 1993. Aq. Micro. Ecol. 12:263-73.
117. Richardson, L. E. 1999. Physical and Chemical Environmental Factors Associated with the Temporal and Spatial Distribution of Cyanobacteria in Lake George, New York. Master's Thesis, Rensselaer Polytechnic Institute, Troy, 111 pp.
118. Rivkin, R. B., Phinney, D. A. & Yentsch, C. M. 1986. Effects of flow cytometric analysis and cell sorting on photosynthetic carbon uptake by phytoplankton in cultures and from natural populations. Appl. Environ. Microbiol. 52:935-8.
119. Robins, D. B. & Bedo, A. W. 1994. Quantitative determination of particle concentrations in experimental and marine environmental samples. Cytometry 17:179-84.
120. Rosenblatt, J. I., Hokanson, J. A., McLaughlin, S. R. & Leary, J. F. 1997. Theoretical basis for sampling statistics useful for detecting and isolating rare cells using flow cytometry and cell sorting. Cytometry 27:233-8.
121. Scanlan, D. J., Silman, N. J., Donald, K. M., Wilson, W. H., Carr, N. G., Joint, I. & Mann, N. H. 1997. An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton. Appl. Environ. Microbiol. 63:2411-20.
122. Schwartz, A. & Fernandez-Repollet, E. 1994. Standardization for flow cytometry. Methods Cell Biol. 42:605-26.
123. Sensen, C. W., Heimann, K. & Melkonian, M. 1993. The production of clonal and axenic cultures of microalgae using fluorescence-activated cell sorting. Eur. J. Phycol. 28:93-7.
124. Shalapyonok, A., Olson, R. J. & Shalapyonok, L. S. 1998. Ultradian growth in Prochlorococcus spp. Appl. Environ. Microbiol. 64:1066-9.
125. Shapiro, H. 1995. Practical Flow Cytometry 3rd ed. Wiley-Liss, New York, 542 pp.
126. 12-starved Euglena gracilis. J. Protozool. 26:626-30.
127. Simon, N., Barlow, R. G., Marie, D., Partensky, F. & Vaulot, D. 1994. Characterization of oceanic photosynthetic picoeukaryotes by flow cytometry. J. Phycol. 30:922-35.
128. Simon, N., LeBot, N., Marie, D., Partensky, F. & Vaulot, D. 1995. Fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes to identify small phytoplankton by flow cytometry. Appl. Environ. Microbiol. 61:2506-13.
129. Sosik, H. M, Chisholm, S. W. & Olson, R. J. 1989. Chlorophyll fluorescence from single cells: interpretation of flow cytometric signals. Limnol. Oceanogr. 34:1749-61.
130. Steen, H. B. 1990. Light scattering measurement in an arc lamp-based flow cytometer. Cytometry 11:223-30.
131. _ 1991. Flow cytometry instrumentation. In Demers, S. [Ed.] Particle Analysis in Oceanography. Springer-Verlag, Berlin, pp. 3-29.
132. _ 1992. Noise, sensitivity, and resolution of flow cytometers. Cytometry 18:822-30.
133. Steinkamp, J. A. 1994. Phase-sensitive detection methods for resolving fluorescence emission signals and directly quantifying lifetime. Methods Cell Biol. 42:627-40.
134. Stewart, C. C. & Steinkamp, J. A. 1982. Quantitation of cell concentration using the flow cytometer. Cytometry 2:238-43.
135. Tate, R. L., III. 1986. Importance of autecology in microbial ecology. In Tate, R. L., III [Ed.] Microbial Autecology. John Wiley & Sons, New York, pp. 1-26.
136. Thomas, J. -C., Desrosiers, M., St-Pierre, Y., Lirette, P., Bisaillon, J. -G., Beaudet, R. & Villemur, R. 1997. Quantitative flow cytometric detection of specific microorganisms in soil samples using rRNA targeted fluorescent probes and ethidium bromide. Cytometry 27:224-32.
137. Toledo, G. & Palenik, B. 1997. Synechococcus diversity in the California current as seen by RNA polymerase (rpoC1) gene sequences of isolated strains. Appl. Environ. Microbiol. 63:4298-303.
138. Trask, B. J., van den Engh, G. J. & Elgershuizen, J. H. B. W. 1982. Analysis of phytoplankton by flow cytometry. Cytometry 2:258-64.
139. Troussellier, M., Courties, C. & Vaquer, A. 1993. Recent applications of flow cytometry in aquatic microbial ecology. Biol. Cell. 78:111-21.
140. Urbach, E. & Chisholm, S. W. 1998. Genetic diversity in Prochlorococcus populations flow cytometrically sorted from the Sargasso Sea and Gulf Stream. Limnol. Oceanogr. 43:1615-30.
141. Urbach, E., Scanlan, D. J., Distel, D. L., Waterbury, J. B. & Chisholm, S. W. 1998. Rapid diversification of marine picophytoplankton with dissimilar light-harvesting structures inferred from sequences of Prochlorococcus and Synechococcus (Cyanobacteria). J. Mol. Evol. 46:188-201.
142. Vaulot, D. & Chisholm, S. W. 1987. Flow cytometric analysis of spermatogenesis in the diatom Thalassiosira weissflogii (Bacillariophyceae). J. Phycol. 23:132-7.
143. Vaulot, D., Courties, C. & Partensky, F. 1989. A simple method to preserve oceanic phytoplankton for flow cytometric analyses. Cytometry 10:629-35.
144. Vaulot, D., LeBot, N., Marie, D. & Fukai, E. 1996. Effect of phosphorus on the Synechococcus cell cycle in surface Mediterranean waters during summer. Appl. Environ. Microbiol. 62:2527-33.
145. Vaulot, D. & Marie, D. 1999. Diel variability of photosynthetic picoplankton in the equatorial Pacific. J. Geophys. Res. 104:3297-310.
146. Vaulot, D., Marie, D., Olson, R. J. & Chisholm, S. W. 1995. Growth of Prochlorococcus, a photosynthetic prokaryote, in the equatorial Pacific Ocean. Science 268:1480-2.
147. Veldhuis, M. J. W., Cucci, T. L. & Sieracki, M. E. 1997. Cellular DNA content of marine phytoplankton using two new fluorochromes: taxonomic and ecological implications. J. Phycol. 33:527-41.
148. Veldhuis, M. J. W. & Kraay, G. W. 1993. Cell abundance and fluorescence of picoplankton in relation to growth irradiance and nitrogen availability in the Red Sea. Neth. J. Sea Res. 31:135-45.
149. Veldhuis, M. J. W., Kraay, G. W. & Gieskes, W. W. C. 1993. Growth and fluorescence characteristics of ultraplankton on a north-south transect in the eastern North Atlantic. Deep Sea Res. II 40:609-26.
150. Vrieling, E. G. & Anderson, D. M. 1996. Immunofluorescence in phytoplankton research: applications and potential. J. Phycol. 32:1-16.
151. Vrieling, E. G., Draaijer, A., van Zeijl, W. J. M., Peperzak, L., Gieskes, W. W. C. & Veenhuis, M. 1993. The effect of labeling intensity, estimated by real-time confocal laser scanning microscopy, on flow cytometric appearance and identification of immunochemically labeled marine dinoflagellates. J. Phycol. 29:180-8.
152. Vrieling, E. G., van de Poll, W. H., Vriezekolk, G. & Gieskes, W. W. C. 1997. Immuno-flow cytometric detection of the ichthyotoxic dinoflagellates Gyrodinium aureolum and Gymnodinium nagasakiense: independence of physiological state. Neth. J. Sea Res. 37:91-100.
153. Weaver, J. C., Bliss, J. G., Powell, K. T., Harrison, G. I. & Williams, G. B. 1991. Rapid clonal growth measurements at the single-cell level: gel microdroplets and flow cytometry. Bio/Technology 9:873-7.
154. Weisse, T. 1993. Dynamics of autotrophic picoplankton in marine and freshwater ecosystems. Adv. Microb. Ecol. 13:327-70.
155. Weisse, T. & Kirchhoff, B. 1997. Feeding of the heterotrophic freshwater dinoflagellate Peridiniopsis berolinense on cryptophytes: analysis by flow cytometry and electronic particle counting. Aq. Micro. Ecol. 12:153-64.
156. Whiteley, A. S., Burkhill, P. H. & Sleigh, M. A. 1993. Rapid method for cell cycle analysis in a predatory marine dinoflagellate. Cytometry 14:909-15.
157. Wilkins, M. F., Boddy, L., Morris, C. W. & Jonker, R. 1996. A comparison of some neural and non-neural methods for identification of phytoplankton from flow cytometry data. Comp. Appl. Biosci. 12:9-18.
158. _ 1999. Identification of phytoplankton from flow cytometry data by using radial basis function neural networks. Appl. Environ. Microbiol. 65:4404-10.
159. Yentsch, C. M. & Campbell, J. W. 1991. Phytoplankton growth: perspectives gained by flow cytometry. J. Plankton Res. 13:S83-S108.