Flow cytometric analyses of viral infection in two marine phytoplankton species, Micromonas pusilla (Prasinophyceae) and Phaeocystis pouchetii (Prymnesiophyceae)

Journal of Phycology

Volumn 35, Issue 5, 1999, Pages 941-948

  Brussaard, Corina P D ^a   Thyrhaug, Runar ^a   Marie, Dominique ^b   Bratbak, Gunnar ^a  

^a UNIVERSITY OF BERGEN   (Norway)

^b STATION BIOLOGIQUE   (France)

Author keywords

Algal viral infection; Flow cytometry; Micromonas pusilla; Phaeocystis pouchetii; Phytoplankton cell characteristics

Indexed keywords

ALGAE; EUKARYOTA; HAPTOPHYCEAE; MICROMONAS PUSILLA; PHAEOCYSTIS POUCHETII; PRASINOPHYCEAE;

EID: 0032730723     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1529-8817.1999.3550941.x     Document Type: Article

References (33)

1. Baumann, M. E. M., Lancelot, C., Brandini, F. P., Sakshaug, E. & John, D. M. 1994. The taxonomic identity of the cosmopolitan prymnesiophyte Phaeocystis: a morphological and ecophysiological approach. J. Mar. Syst. 5:5-22.
2. Benson, R. & Martin, E. 1981. Effects of photosynthetic inhibitors and light-dark regimes on the replication of cyanophages SM-2. Arch. Microbiol. 129:165-7.
3. Bratbak, G., Egge, J. K. & Heldal, M. 1993. Viral mortality of the marine alga Emiliania huxleyi (Haptophyceae) and termination of algal blooms. Mar. Ecol. Prog. Ser. 93:39-48.
4. Bratbak, G. & Heldal, M. 1993. Total count of viruses in aquatic environments In Kemp, P. F., Sherr, B. F., Sherr, E. B. & Cole, J. J. [Eds.] Current Methods in Aquatic Microbial Ecology. Lewis Publishers, London, pp. 135-8.
5. Bratbak, B., Heldal, M., Jacobsen, A., Nagasaki, K. & Thingstad, F. 1998. Virus production in Phaeocystis pouchetii and its relation to host cell growth and nutrition. Aquat. Microbiol. Ecol. 16:1-9.
6. Bratbak, G., Thingstad, F. & Heldal, M. 1994. Viruses and the microbial loop. Microb. Ecol. 28: 209-21.
7. Brussaard, C. P. D., Kempers, R. S., Kop, A. J., Riegman, R. & Heldal, M. 1996. Virus like particles in a summer bloom of Emiliania huxley in the North Sea. Aquat. Microb. Ecol. 10:105-13.
8. Chirura, H. X. 1997. Generalized gene transfer by virus-like particles from marine bacteria. Aquat. Microb. Ecol. 13:75-83.
9. Cottrell, M. T. & Suttle, C. A. 1991. Wide-spread occurrence and clonal variation in viruses which cause lysis of a cosmopolitan eukaryotic marine phytoplankter, Micromonas pusilla. Mar. Ecol. Prog. Ser. 78:1-9.
10. _ 1995. Dynamics of a lytic virus infecting the photosynthetic marine picoflagellate Micromonas pusilla. Limnol. Oceanogr. 40:730-9.
11. Fields, B. N., Knipe, D. M., & Howley, P. M. [Eds.] 1996. Fundamental Virology, 3rd ed. Lippincott-Raven Publishers, Philadelphia, Pennsylvania, 1340 pp.
12. Fuhrman, J. A. & Suttle, C. A. 1993. Viruses in marine planktonic systems. Oceanography 6:51-63.
13. Gobler, C. J., Hutchins, D. A., Fisher, N. S., Cosper, E. M. & Sañ udo-Wilhemy, S. A. 1997. Release and bioavailability of C, N, P, Se, and Fe following viral lysis of a marine chrysophyte. Limnol. Oceanogr. 42:1492-1504.
14. Guillard, R. R. L. 1975. Culture of phytoplankton for feeding marine invertebrates. In Smith, W. L. & Chanley, M. H. [Eds.] Culture of Marine Invertebrate Animals. Plenum Press, New York, pp. 29-60.
15. Harrison, P. J., Waters, R. E. & Taylor, F. J. R. 1980. A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J. Phycol. 16:28-35.
16. Hill, R. W., White, B. A., Cottrell, M. T. & Dacey, J. W. H. 1998. Virus-mediated total release of dimethylsulfoniopropionate from marine phytoplankton: a potential climate process. Aquat. Microb. Ecol. 14:1-6.
17. Jacobsen, A., Bratbak, G. & Heldal, M. 1996. Isolation and characterization of a virus infecting Phaeocystis pouchetii (Prymnesiophyceae). J. Phycol. 32:923-7.
18. Levy, J. A., Fraenkel-Conrat, H. & Owens, R. A. [Eds.] 1994. Virology, 3rd ed. Prentice Hall, Englewood Cliffs, New Jersey, 447 pp.
19. Luria, S. E. [Ed.] 1958. General Virology. J. Wiley, New York, 427pp.
20. Marie, D., Brussaard, C. P. D., Thyrhaug, R., Bratbak, G. & Vaulot, D. 1999. Enumeration of viruses in marine samples by flow cytometry. Appl. Environ. Microbiol. 65:45-52.
21. Mayer, J. A. & Taylor, F. J. R. 1979. A virus which lyses the marine nanoflagellate Micromonas pusilla. Nature 281:299-301.
22. Meints, R. H., Lee, K. & Van Etten, J. L. 1986. Assembly of the virus PBCV-1 in a Chlorella-like green algae: ultrastructural studies. Virology 154:240-5.
23. Nagasaki, K., Ando, M., Itakura, S., Imai, I. & Ishida, Y. 1994. Viral mortality in the final stage of Heterosigma akashiwo (Raphidophyceae) red tide. J. Plankton Res. 16:1595-9.
24. Owens, M. A. & Loken, M. R. [Eds.] 1995. Flow Cytometric Principles for Clinical Laboratory Practice. Wiley-Liss, New York, 224 pp.
25. Pienaar, R. N. 1976. Virus-like particles in three species of phytoplankton from San Juan Island, Washington. Phycologia 15: 185-90.
26. Shapiro, H. M. [Ed.] 1988. Practical Flow Cytometry, 2nd ed. Wiley-Liss, New York, 353 pp.
27. Stent, G. S. [Ed.] 1963. Molecular Biology of Bacterial Viruses. W. H. Freeman, San Francisco, 474 pp.
28. Suttle, C. A. & Chan, A. M. 1993. Marine cyanophages infecting oceanic and coastal strains of Synechococcus abundance, morphology, cross-infectivity and growth characteristics. Mar. Ecol. Prog. Ser. 92:99-109.
29. Suttle, C. A., Chan, A. M. & Cottrell, M. T. 1990. Infection of phytoplankton by viruses and reduction of primary productivity. Nature 347:467-9.
30. Van Etten, J. L., Lane, L. C. & Meints, R. H. 1991. Viruses and viruslike particles of eukaryotic algae. Microbiol. Rev. 55:586-620.
31. Vaulot, D., Courties, C. & Partensky, F. 1989. A simple method to preserve oceanic phytoplankton for flow cytometric analyses. Cytometry 10:629-35.
32. Vaulot, D., Birrien, J-L., Marie, D., Casotti, R., Veldhuis, M. J. W., Kraay, G. W. & Chrétiennot-Dinet, M-J. 1994. Morphology, ploidy, pigment composition, and genome size of cultured strains of Phaeocystis (Prymnesiophyceae). J. Phycol. 30:1022-35.
33. Waters, R. E. & Chan, A. T. 1982. Micromonas pusilla virus: the virus growth cycle and associated physiological events within the host cells; host range mutation. J. Gen. Virol. 63:199-206.